Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment

Analytical Biochemistry

Volumn 410, Issue 2, 2011, Pages 214-223

  Sonomura, Kazuhiro ^a,b   Kuyama, Hiroki ^a   Matsuo, Ei Ichi ^a,b   Tsunasawa, Susumu ^a   Futaki, Shiroh ^c   Nishimura, Osamu ^a  

^a OSAKA UNIVERSITY   (Japan)

^b SHIMADZU CORPORATION   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Endoproteinase AspN; MALDI TOF MS; N blocked protein; N terminal sequence analysis; Tosylhydrazine; Transamination

Indexed keywords

MASS SPECTROMETRY; SILICA;

ENDOPROTEINASE ASPN; MALDI TOF MS; N-TERMINAL SEQUENCES; TOSYLHYDRAZINE; TRANSAMINATION;

PEPTIDES;

ASPARTIC ACID; CARBONYL DERIVATIVE; GLASS; HYDRAZINE DERIVATIVE; TOSYLHYDRAZINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEOMICS; TRANSAMINATION;

EUKARYOTA;

EID: 79551520250     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.12.006     Document Type: Article

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