A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic nanoparticles and MS

Proteomics

Volumn 9, Issue 18, 2009, Pages 4416-4420

  Zhao, Liyan ^a   Zhang, Yangjun ^a   Wei, Junying ^a   Cao, Dong ^a   Liu, Kehui ^a   Qian, Xiaohong ^a  

^a BEIJING INSTITUTE OF RADIATION MEDICINE   (China)

Author keywords

HepG2 cells; Isothiocyanate coupled magnetic nanoparticles; MS; N blocked peptides; Technology

Indexed keywords

ISOTHIOCYANIC ACID; NANOPARTICLE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL LYSATE; CELL STRAIN HEPG2; CONTROLLED STUDY; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; HUMAN CELL; MAGNETIC FIELD; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ISOLATION;

CELL LINE, TUMOR; CHROMATOGRAPHY, ION EXCHANGE; FERROSOFERRIC OXIDE; HUMANS; ISOTHIOCYANATES; METAL NANOPARTICLES; MICROSCOPY, ELECTRON, TRANSMISSION; PEPTIDES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 70349125874     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200800879     Document Type: Article

References (17)

1. Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K. et al., Protein identification with N- and C-terminal sequence tags in proteome projects. J. Mol. Biol. 1998, 278, 599-608. (Pubitemid 28224857)
2. Nakazawa, T., Yamaguchi, M., Okamura, T. A., Ando, E. et al., Terminal proteomics: N- and C-terminal analyses for high-fidelity identification of proteins using MS. Proteomics 2008, 8, 673-685. (Pubitemid 351362929)
3. Meinnel, T., Giglione, C., Tools for analyzing and predicting N-terminal protein modifications. Proteomics 2008, 8, 626-649. (Pubitemid 351362927)
4. Betancourt, L., Besada, V., González, L. J., Morera, V., Selective isolation and identification of N-terminal blocked peptides from tryptic protein digests. J. Pept. Res. 2001, 57, 345-353. (Pubitemid 32769039)
5. Dormeyer, W., Mohammed, S., Breukelen, B., Krijgsveld, J. et al., Targeted analysis of protein termini. J. Proteome Res. 2007, 6, 4634-4645.
6. Gevaert, K., Goethals, M., Martens, L., Van Damme, J. et al., Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat. Biotechnol. 2003, 21, 566-569. (Pubitemid 36532024)
7. Falb, M., Aivaliotis, M., Garcia-Rizo, C., Bisle, B. et al., Archaeal N-terminal protein maturation commonly involves N-terminal acetylation: a large-scale proteomics survey. J. Mol. Biol. 2006, 362, 915-924. (Pubitemid 44353516)
8. Coussot, G., Hawke, D. H., Mularz, A., Koomen, J. M. et al., A method for the isolation of blocked N-terminal peptides. Anal. Biochem. 2007, 361, 302-304. (Pubitemid 46135392)
9. Mikami, T., Takao, T., Selective isolation of N-blocked peptides by isocyanate-coupled resin. Anal. Chem. 2007, 79, 7910-7915. (Pubitemid 47622424)
10. Shen, P. T., Hsu, J. L., Chen, S. H., Dimethyl isotope-coded affinity selection for the analysis of free and blocked N-termini of proteins using LC-MS/MS. Anal. Chem. 2007, 79, 9520-9530.
11. Tan, F., Zhang, Y., Mi, W., Wang, J. et al., Enrichment of phosphopeptides by Fe31-immobilizedmagnetic nanoparticles for phosphoproteome analysis of the plasma membrane of mouse liver. J. Proteome Res. 2008, 7, 1078-1087.
12. Wei, J. Y., Zhang, Y. J., Tan, F., Qian, X. H., Highly efficient enrichment of phosphopeptides by magnetic nanoparticles coated with zirconium phosphonate for phosphoproteome analysis. Rapid Commun. Mass Spectrom. 2008, 22, 1069-1080.
13. Beardsley, R. L., Karty, J. A., Reilly, J. P., Enhancing the intensities of lysine-terminated tryptic peptide ions in matrixassisted laser desorption/ionizationmass spectrometry. Rapid Commun. Mass Spectrom. 2000, 14, 2147-2153.
14. Beardsley, R. L., Reilly, J. P., Optimization of guanidination procedures for MALDI mass mapping. Anal. Chem. 2002, 74, 1884-1890.
15. Thiede, B., Lamer, S., Mattow, J., Siejak, F. et al., Analysis of missed cleavage sites, tryptophan oxidation and N-terminal pyroglutamylation after in-gel tryptic digestion. Rapid Commun. Mass Spectrom. 2000, 14, 496-502. (Pubitemid 30153000)
16. Geoghegan, K. F., Hoth, L. R., Tan, D. H., Borzilleri, K. A. et al., Cyclization of N-terminal S-carbamoylmethylcysteine causing loss of 17Da from peptides and extra peaks in peptide maps. J. Proteome Res. 2002, 1, 181-187.
17. Martens, L., Vandekerckhove, J., Gevaert, K., DBToolkit: processing protein databases for peptide-centric proteomics. Bioinformatics 2005, 21, 3584-3585. (Pubitemid 41236010)