Cloning, expression and purification of microcystis viridis lectin in Escherichia coli

Molecular Biotechnology

Volumn 47, Issue 2, 2011, Pages 105-110

  Li, Yuqin ^a,b   Liao, Xiaoxia ^a   Chen, Gu ^a   Yap, Yeeleng ^c   Zhang, Xuewu ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b XIANGTAN UNIVERSITY   (China)

^c DAVOS LIFE SCIENCE PTE LTD   (Singapore)

Author keywords

Cloning; E. coli; Expression; Purification

Indexed keywords

ANTI-HIV ACTIVITY; BLUE GREEN ALGAE; COST-EFFECTIVE PRODUCTION; E. COLI; EXPRESSION; INCLUSION BODIES; MALDI-TOF-MS ANALYSIS; MICROCYSTIS; ONE-STEP PURIFICATION; PROTEIN IDENTIFICATION; SHAKE-FLASK CULTURES; SOLUBLE FRACTION; SUGAR-BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; ALGAE; CELL CULTURE; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; PROTEINS; SUGARS;

PURIFICATION;

CARBOHYDRATE BINDING PROTEIN; MICROCYSTIS VIRIDIS LECTIN; NICKEL; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; CELL CULTURE; CONTROLLED STUDY; ESCHERICHIA COLI; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROCYSTIS; MICROCYSTIS VIRIDIS; MOLECULAR CLONING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

BASE SEQUENCE; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; GENE EXPRESSION; LECTINS; MICROCYSTIS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

CHLOROPHYTA; ESCHERICHIA COLI; MICROCYSTIS VIRIDIS;

EID: 79551510063     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9315-0     Document Type: Article

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