Expression, purification, and characterization of recombinant cyanovirin-N for vaginal anti-HIV microbicide development

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 229-236

  Colleluori, Diana M ^a   Tien, Deborah ^a   Kang, Feirong ^a   Pagliei, Tara ^a   Kuss, Ryan ^a   McCormick, Timothy ^a   Watson, Karen ^b   McFadden, Karyn ^c   Chaiken, Irwin ^c   Buckheit Jr , Robert W ^b   Romano, Joseph W ^a  

^a Adamis Pharmaceuticals Corporation   (United States)

^b ImQuest Biosciences   (United States)

^c DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

anti HIV; CV N; Cyanobacteria; Cyanovirin N; HIV; Microbicide

Indexed keywords

ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT; BACTERIAL PROTEIN; CARRIER PROTEIN; CYANOVIRIN N; GLYCOPROTEIN GP 120; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; DRUG EFFECT; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME STABILITY; ESCHERICHIA COLI; FEMALE; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENE VECTOR; GENETICS; HELA CELL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IC 50; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; VAGINA; VIRUS INCLUSION; WESTERN BLOTTING;

ANIMALS; ANTI-HIV AGENTS; BACTERIAL PROTEINS; BLOTTING, WESTERN; CARRIER PROTEINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; GENETIC VECTORS; HELA CELLS; HIV ENVELOPE PROTEIN GP120; HUMANS; INCLUSION BODIES, VIRAL; INHIBITORY CONCENTRATION 50; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; VAGINA;

CYANOBACTERIA; ESCHERICHIA COLI; PROKARYOTA;

EID: 19944427993     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.10.009     Document Type: Article

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