PCR-based community structure studies of Bacteria associated with eukaryotic organisms: A simple PCR strategy to avoid co-amplification of eukaryotic DNA

Journal of Microbiological Methods

Volumn 84, Issue 2, 2011, Pages 349-351

  Bakke, Ingrid ^a   De Schryver, Peter ^b   Boon, Nico ^b   Vadstein, Olav ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Bacterial SSU rDNA; Biased PCR; Co amplification; DGGE; Eukaryotic SSU rDNA; Nested PCR

Indexed keywords

BACTERIAL DNA; EUKARYOTIC DNA; RIBOSOME DNA; RIBOSOME RNA; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; CONTROLLED STUDY; EUKARYOTE; GENE AMPLIFICATION; LARVA; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA SEQUENCE; SEQUENCE ALIGNMENT;

BACTERIA; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; EUKARYOTA; METAGENOMICS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 79251611215     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.12.015     Document Type: Article

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