Use of signature-tagged mutagenesis to identify virulence determinants in Haemophilus ducreyi responsible for ulcer formation

Journal of Microbiological Methods

Volumn 84, Issue 2, 2011, Pages 290-298

  Yeung, Angela ^a   Cameron, D William ^a   Desjardins, Marc ^a   Lee, B Craig ^a  

^a UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Chancroid; Haemophilus ducreyi; Signature tagged mutagenesis; Transposon

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL METABOLISM; CONTROLLED STUDY; DNA REPAIR; GENE CONTROL; GENE INSERTION; GENE MUTATION; GENETIC SCREENING; HAEMOPHILUS DUCREYI; HBGA GENE; HICB GENE; HIGH THROUGHPUT SCREENING; LSPA1 GENE; MICROBIAL ACTIVITY; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RABBIT; TEMPERATURE DEPENDENCE; ULCUS MOLLE; VIRUS VIRULENCE;

ANIMALS; CHANCROID; DISEASE MODELS, ANIMAL; HAEMOPHILUS DUCREYI; MALE; MUTAGENESIS, INSERTIONAL; RABBITS; VIRULENCE; VIRULENCE FACTORS;

HAEMOPHILUS DUCREYI; ORYCTOLAGUS CUNICULUS;

EID: 79251601425     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.12.022     Document Type: Article

References (50)

1. Albritton W.L. Biology of Haemophilus ducreyi. Microbiol. Rev. 1989, 53:377-389.
2. Al-Tawfiq J.A., Fortney K.R., Katz B.P., Elkins C., Spinola S.M. An isogenic hemoglobin receptor-deficient mutant of Haemophilus ducreyi is attenuated in the human model of experimental infection. J. Infect. Dis. 2000, 181:1049-1054.
3. Bauer M.E., Townsend C.A., Ronald A.R., Spinola S.M. Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Microb. Infect. 2006, 8:2465-2468.
4. Bauer M.E., Fortney K.R., Harrison A., Janowicz D.M., Munson R.S., Spinola S.M. Identification of Haemophilus ducreyi genes expressed during human infection. Microbiology 2008, 154:1152-1160.
5. Blackmore C.A., Limpakarnjanarat K., Rigau-Perez J.G., Albritton W.L., Greenwood J.R. An outbreak of chancroid in Orange County, California: descriptive epidemiology and disease-control measures. J. Infect. Dis. 1985, 151:840-844.
6. Cameron D.W., Simonsen J.N., D'Costa L.J., Ronald A.R., Maitha G., Gakinya M.N., Cheang M., Ndinya-Achola J.O., Piot P., Brunhan R.C., Plummer F.A. Female to male transmission of human immunodeficiency virus type 1: risk factors for seroconversion in men. Lancet 1989, ii:403-407.
7. Chiang S.L., Mekalanos J.J. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol. Microbiol. 1998, 27:797-805.
8. Chiang S.L., Mekalanos J.J., Holden D.W. In vivo genetic analysis of bacterial virulence. Annu. Rev. Microbiol. 1999, 53:129-154.
9. Cope L.D., Lumbley S., Latimer J.L., Klesney-Tait J., Stevens M.K., Johnson L.S., Purven M., Munson R.S., Lagergard T., Radolf J.D., Hansen E.J. A diffusible cytotoxin of Haemophilus ducreyi. Proc. Natl. Acad. Sci. U. S. A. 1997, 94:4056-4061.
10. Coulter S.N., Schwan W.R., Ng E.Y.W., Langhorne M.H., Ritchie H.D., Westbrock-Wadman S., Hufnagle W.O., Folger K.R., Bayer A.S., Stover C.K. Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments. Mol. Microbiol. 1998, 30:393-404.
11. Darwin A.J., Miller V.L. Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis. Mol. Microbiol. 1999, 32:51-62.
12. Desjardins M., Filion L.G., Robertson S., Cameron D.W. Inducible immunity with a pilus preparation booster vaccination in an animal model of Haemophilus ducreyi infection and disease. Infect. Immun. 1995, 63:2012-2020.
13. Dillon S.M., Cummings M., Rajagopalan S., McCormack W.C. Prospective analysis of genital ulcer disease in Brooklyn, New York. Clin. Infect. Dis. 1997, 24:945-950.
14. Edelstein P.H., Edelstein M.A.C., Higa F., Falkow S. Discovery of virulence genes of Legionella pneumophila by using signature tagged mutagenesis in a guinea pig pneumonia model. Proc. Natl. Acad. Sci. U. S. A. 1999, 96:8190-8195.
15. Elkins C., Chen C.-J., Thomas C.E. Characterization of the hgbA locus encoding a hemoglobin receptor from Haemophilus ducreyi. Infect. Immun. 1995, 63:2194-3200.
16. Finlay B.B., Falkow S. Common themes in microbial pathogenicity revisited. Microbiol. Mol. Biol. Rev. 1997, 61:136-169.
17. Fleming D.T., Wasserheit J.N. From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection. Sex. Transm. Infect. 1999, 75:3-17.
18. Fortney K., Totten P.A., Lehrer R.L., Spinola S.M. Haemophilus ducreyi is susceptible to protegrin. Antimicrob. Agents Chemother. 1998, 42:2690-2693.
19. Gerdes K., Christensen S.K., Lobner-Olesen A. Prokaryotic toxin-antitoxin stress response loci. Nat. Rev. Microbiol. 2005, 3:371-382.
20. Hammond G.W., Slutchuk M., Scatliff J., Sherman E., Wilt J.C., Ronald A.R. Epidemiologic, clinical, laboratory, and therapeutic features of an urban outbreak of chancroid in North America. Rev. Infect. Dis. 1980, 2:867-879.
21. Handfield M., Levesque R.C. Strategies for isolation of in vivo expressed genes from bacteria. F.E.M.S. Microbiol. Rev. 1999, 23:69-91.
22. Hava D., Camilli A. Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 2002, 45:1389-1406.
23. Haydock A.K., Martin D.H., Morse S.A., Cammarata C., Mertz K.J., Totten P.A. Molecular characterization of Haemophilus ducreyi strains from Jackson, Mississippi, and New Orleans, Louisiana. J. Infect. Dis. 1999, 179:1423-1432.
24. Hensel M., Shea J.E., Gleeson C., Jones M.D., Dalton E., Holden D.W. Simultaneous identification of bacterial virulence genes by negative selection. Science 1995, 269:400-403.
25. Hobbs M.M., San Mateo L.R., Orndorff P.E., Almond G., Kawula T.H. Swine model of Haemophilus ducreyi infection. Infect. Immun. 1995, 63:3094-4100.
26. Janowicz D.M., Fortney K.R., Katz B.P., Latimer J.L., Deng K., Hansen E.J., Spinola S.M. Expression of the LspA1 and LspA2 proteins by Haemophilus ducreyi is required for virulence in human volunteers. Infect. Immun. 2004, 72:4528-4533.
27. Janowicz D.M., Ofner S., Katz B.P., Spinola S.M. Experimental infection of human volunteers with Haemophilus ducreyi: fifteen years of clinical data and experience. J. Infect. Dis. 2009, 199:1671-1679.
28. Janowicz D.M., Li W., Bauer M.E. Host-pathogen interplay of Haemophilus ducreyi. Curr. Opin. Infect. Dis. 2010, 23:4-9.
29. Johnson S.R., Martin D.H., Cammarata C., Morse S.A. Development of a polymerase chain reaction assay for the detection of Haemophilus ducreyi. Sex. Transm. Dis. 1994, 21:13-23.
30. Kreiss J.K., Koech D., Plummer F.A., Holmes K.K., Lightfoote M., Piot P., Ronald A.R., Ndinya-Achola J.O., D'Costa L.J., Roberts P., Ngugi E.N., Quinn T.C. AIDS virus infection in Nairobi prostitutes: spread of the epidemic to East Africa. N. Engl. J. Med. 1986, 314:414-418.
31. Leduc I., Banks K.E., Fortney K.R., Patterson K.B., Billings S.D., Katz B.P., Spinola S.M., Elkins C. Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. J. Infect. Dis. 2008, 197:1103-1109.
32. Lehoux D.E., Sanschagrin F., Levesque R.C. Defined oligonucleotide tag pools and PCR screening in signature-tagged mutagenesis of essential genes from bacteria. Biotechniques 1999, 26:473-480.
33. MacDonald K.C., Cameron D.W., D'Costa L.J., Ndinya-Achola J.O., Plummer F.A., Ronald A.R. Evaluation of fleroxacin (RO23-6240) as single dose therapy of culture-proven chancroid in Nairobi, Kenya. Antimicrob. Agents Chemother. 1989, 33:612-614.
34. Martin D.H., DiCarlo R.P. Recent changes in the epidemiology of genital ulcer disease in the United States. The crack cocaine connection. Sex. Transm. Dis. 1994, 21(2Suppl):S76-S80.
35. Mei J.-M., Nourbakhsh F., Ford C.W., Holden D.W. Identification of Staphylococcus aureus virulence genes in a murine model of bacteremia using signature-tagged mutagenesis. Mol. Microbiol. 1997, 26:399-407.
36. Morse S.A. Chancroid and Haemophilus ducreyi. Clin. Microbiol. Rev. 1989, 2:137-157.
37. Mount K.L.B., Townsend C.A., Rinker S.D., Gu X., Fortney K.R., Zwickl B.W., Janowicz D.M., Spinola S.M., Katz B.P., Bauer M.E. Haemophilus ducreyi SapA contributes to cathelicidin resistance and virulence in humans. Infect. Immun. 2010, 78:1176-1184.
38. Peschel A. How do bacteria resist human antimicrobial peptides?. Trends Microbiol. 2002, 10:179-185.
39. Plummer F.A., Simonsen J.N., Cameron D.W., Ndinya-Achola J.O., Kreiss J.K., Gakinya M.N., Waiyaki P., Cheang M., Piot P., Ronald A.R., Ngugi E.N. Cofactors in male-female sexual transmission of human immunodeficiency virus type 1. J. Infect. Dis. 1991, 163:233-239.
40. Polissi A., Pontiggia A., Ferger G., Altieri M., Mottl H., Ferrari L., Simon D. Large-scale identification of virulence genes from Streptococcus pneumoniae. Infect. Immun. 1998, 66:5620-5629.
41. Purcell B.K., Richardson J.A., Radolf J.D., Hansen E.J. A temperature-dependent rabbit model for production of dermal lesions by Haemophilus ducreyi. J. Infect. Dis. 1991, 164:359-367.
42. Purven M., Lagergard T. Haemophilus ducreyi, a cytotoxin-producing bacterium. Infect. Immun. 1992, 60:1156-1162.
43. Rediers H., Rainey P.B., Vanderleyden J., de Mot R. Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps for exploring niche-specific gene expression. Microbiol. Mol. Biol. Rev. 2005, 69:217-261.
44. Stevens M.K., Cope L.D., Radolf J.D., Hansen E.J. A system for generalized mutagenesis of Haemophilus ducreyi. Infect. Immun. 1995, 63:2976-2982.
45. Totten P.A., Morton W.R., Knitter G.H., Clark A.M., Kiviat N.B., Stamm W.E. A primate model for chancroid. J. Infect. Dis. 1994, 169:1284-1290.
46. Tyndall M., Malisa M., Plummer F.A., Ombetti J., Ndinya-Achola J.O., Ronald A.R. Ceftriaxone no longer predictably cures chancroid in Kenya. J. Infect. Dis. 1993, 167:469-471.
47. van Diemen P.M., Dziva F., Stevens M.P., Wallis T.S. Identification of enterohemorrhagic Escherichia coli 028:H genes requires for intestinal colonization in calves. Infect. Immun. 2005, 71:2478-2486.
48. van Melderen L., de Bast M.S. Bacterial toxin-antitoxin systems more than selfish entities?. PLoS Genet. 2009, 5:e1000437.
49. Wasserheit J.N. Epidemiological synergy: interrelationships between human immunodeficiency virus infection and other sexually transmitted diseases. Sex. Transm. Dis. 1992, 19:61-77.
50. Zasloff M. Antimicrobial peptides of multicellular organisms. Nature 2002, 415:389-395.