Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA

Development Growth and Differentiation

Volumn 53, Issue 1, 2011, Pages 69-75

  Hou, Xubin ^a   Omi, Minoru ^a   Harada, Hidekiyo ^a   Ishii, Shunsuke ^b   Takahashi, Yoshiko ^c   Nakamura, Harukazu ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b RIKEN TSUKUBA INSTITUTE   (Japan)

^c NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Electroporation; Pol II transcription; SiRNA; Tet on

Indexed keywords

DOUBLE STRANDED RNA; GREEN FLUORESCENT PROTEIN; RNA POLYMERASE II; SMALL INTERFERING RNA; TETRACYCLINE;

ARTICLE; CHICK; ELECTROPORATION; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TRANSFER; GENETIC TRANSCRIPTION; GENOME; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; RNA TRANSCRIPTION; TRANSCRIPTION REGULATION; WESTERN BLOTTING;

ANIMALS; CHICK EMBRYO; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; TETRACYCLINE; TRANSCRIPTION, GENETIC;

EID: 79251505569     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/j.1440-169X.2010.01229.x     Document Type: Article

References (26)

1. Amar, L., Desclaux, M., Faucon-Biguet, N., Mallet, J. & Vogel, R. 2006. Control of small inhibitory RNA levels and RNA interference by doxycycline induced activation of a minimal RNA polymerase III promoter. Nuc Acid Res. 34, e37.
2. Bally-Cuif, L., Cholley, B. & Wassef, M. 1995. Involvement of Wnt-1 in the formation of the mes/metencephalic boundary. Mech. Dev. 53, 23-34.
3. Caplen, N. J., Parrish, S., Imani, F., Fire, A. & Morgan, R. A. 2001. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl Acad. Sci. USA 98, 9742-9747.
4. Czauderna, F., Santel, A., Hinz, M., Fechtner, M., Durieux, B., Fisch, G., Leenders, F., Arnold, W., Giese, K., Klippel, A. & Kaufmann, J. 2003. Inducible shRNA expression for application in a prostate cancer mouse model. Nuc. Acid Res. 31, e127.
5. Desclaux, M., Teigell, M., Amar, L., Vogel, R., Gimenez y Ribotta, M., Privat, A. & Mallet, J. 2009. A novel and efficient gene transfer starategy reduces glial reactivity and improves neuronal survival and axonal growth in vitro. PLoS ONE 4, e6227.
6. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K. & Tuschl, T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.
7. Funahashi, J., Okafuji, T., Ohuchi, H., Noji, S., Tanaka, H. & Nakamura, H. 1999. Role of Pax-5 in the regulation of a mid-hindbrain organizer's activity. Dev. Growth Differ. 41, 59-72.
8. Hammond, S. M., Bernstein, E., Beach, D. & Hannon, G. J. 2000. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404, 293-296.
9. Harborth, J., Elbashir, S. M., Bechert, K., Tuschl, T. & Weber, K. 2001. Identification of essential genes in cultured mammalian cells using small interfering RNAs. J. Cell Sci. 114, 4557-4565.
10. Hou, X., Katahira, T., Kimura, J. & Nakamura, H. 2009. Expression of Chick Coactosin in cells in morphogenetic movement. Dev. Growth Differ. 51, 833-840.
11. Hilgers, V., Pourquié, O. & Dubrulle, J. 2005. In vivo analysis of mRNA stability using the Tet-Off system in the chicken embryo. Dev. Biol. 284, 292-300.
12. Kappel, S., Matthess, Y., Zimmer, B., Kaufmann, M. & Strebhardt, K. 2006. Tumor inhibition by genomically integrated inducible RNAi-cassette. Nuc. Acid Res. 34, 4527-4536.
13. Katahira, T. & Nakamura, H. 2003. Gene silencing in chick embryos with a vector-based small interfering RNA system. Dev. Growth Differ. 45, 361-367.
14. Matsuda, T. & Cepko, C. L. 2007. Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl Acad. Sci. USA 104, 1027-1032.
15. Muramatsu, T., Mizutani, Y., Ohmori, Y. & Okumura, J. 1997. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230, 376-380.
16. Nakamura H. ed. 2009. Electroporation and Sonoporation in Developmental Biology. Springer Japan, Tokyo.
17. Nakamura, H., Katahira, T., Sato, T., Watanabe, Y. & Funahashi, J. 2004. Gain- and loss-of-function in chick embryos by electroporation. Mech. Dev. 121, 1137-1143.
18. Newgreen, D. & Thiery, J. P. 1980. Fibronectin in early avian embryos: synthesis and distribution along the migration pathways of neural crest cells. Cell Tissue Res. 211, 269-291.
19. Odani, N., Ito, K. & Nakamura, H. 2008. Electroporation as an efficient method of gene transfer. Dev. Growth Differ. 50, 443-448.
20. Patel, N. H., Martin-Blanco, E., Coleman, K. G., Poole, S. J., Ellis, M. C., Kornberg, T. B. & Goodman, C. S. 1989. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 58, 955-968.
21. Sato, Y., Kasai, T., Nakagawa, S., Tanabe, K., Watanabe, T., Kawakami, K. & Takahashi, Y. 2007. Stable integration and conditional expression of electroporated transgenes in chicken embryos. Dev. Biol. 305, 616-624.
22. Shinagawa, T. & Ishii, S. 2003. Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter. Genes Dev. 17, 1340-1345.
23. Stern, C. D. 1998. Detection of multiple gene products simultaneously by in situ hybridization and immunohistochemistry in whole mounts of avian embryos. Curr. Top. Dev. Biol. 36, 223-243.
24. Watanabe, T., Saito, D., Tanabe, K., Suetsugu, R., Nakaya, Y., Nakagawa, S. & Takahashi, Y. 2007. Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos. Dev. Biol. 305, 625-636.
25. Yonaha, M. & Proudfoot, N. J. 1999. Specific transcriptional pausing activates polyadenylation in a coupled in vitro system. Mol. Cell 3, 593-600.
26. Yonaha, M. & Proudfoot, N. J. 2000. Transcriptional termination and coupled polyadenylation in vitro. EMBO J. 19, 3770-3777.