Application of conventional and multiplex PCR assays for identification of isolates of leptospira spp. in Colombia;Aplicación de las pruebas de pcr convencional simple y múltiple para la identificación de aislamientos de leptospira spp. en Colombia

Revista Peruana de Medicina Experimental y Salud Publica

Volumn 27, Issue 4, 2010, Pages 548-556

  Moreno, Natali ^a   Agudelo Flórez, Piedad ^a  

^a Instituto Colombiano de Medicina Tropical   (Colombia)

Author keywords

Colombia; Leptospira; Molecular diagnostic techniques; Polymerase chain reaction

Indexed keywords

EID: 78751554176     PISSN: 17264634     EISSN: 17264634     Source Type: Journal    
DOI: 10.1590/S1726-46342010000400009     Document Type: Article

References (32)

1. McBride A, Athanazio D, Reis M, Ko A. Leptospirosis. Curr Opin Infect Dis. 2005;18(5):376-86.
2. Bharti A, Nally J, Ricaldi J, Matthias M, Diaz M, Lovett M, et al. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis. 2003;3(12):757-71.
3. Epstein P, Calix Pena O, Blanco Racedo J. Climate and disease in Colombia. Lancet. 1995;346(8985):1243-44.
4. Agudelo-Flórez P, Restrepo-Jaramillo B, Arboleda- Naranjo M. Leptospirosis in Uraba, Antioquia, Colombia: a seroepidemiological and risk factor survey in the urban population. Cad Saude Publica. 2007;23(9):2094-102.
5. Sebek Z, Sixl W, Valova M, Marth E, Köck M, Reinthaler F. Serological investigations for leptospirosis in humans in Columbia. Geogr Med Suppl. 1989;3:51-60.
6. Ferro B, Rodríguez A, Pérez M, Travi B. Seroprevalence of Leptospira infection in habitants of peripheral neighborhoods in Cali, Colombia. Biomedica. 2006;26(2):250-57.
7. Haake D, Suchard M, Kelley M, Dundoo M, Alt D, Zuerner R. Molecular evolution and mosaicism of leptospiral outer membrane proteins involves horizontal DNA transfer. J Bacteriol. 2004;186(9):2818-28.
8. de la Peña-Moctezuma A, Bulach D, Adler B. Genetic differences among the LPS biosynthetic loci of serovars of Leptospira interrogans and Leptospira borgpetersenii. FEMS Immunol Med Microbiol. 2001;31(1):73-81.
9. Xue F, Yan J, Picardeau M. Evolution and pathogenesis of Leptospira spp.: lessons learned from the genomes. Microbes Infect. 2009;11(3):328-33.
10. Koizumi N, Watanabe H. Leptospirosis vaccines: past, present, and future. J Postgrad Med.51(3):210-4.
11. Ko A, Goarant C, Picardeau M. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol. 2009;7(10):736-47.
12. Kawabata H, Dancel L, Villanueva S, Yanagihara Y, Koizumi N, Watanabe H. flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp. Microbiol Immunol. 2001;45(6):491-96.
13. Lin M, Surujballi O, Nielsen K, Nadin-Davis S, Randall G. Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis. Infect Immun. 1997;65(10):4355-9.
14. Levett P, Morey R, Galloway R, Turner D, Steigerwalt A, Mayer L. Detection of pathogenic leptospires by real-time quantitative PCR. J Med Microbiol. 2005;54(Pt 1):45-9.
15. Dong H, Hu Y, Xue F, Sun D, Ojcius D, Mao Y, et al. Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity of the OmpL1 protein. BMC Microbiol. 2008;8:223.
16. Ahmed A, Engelberts M, Boer K, Ahmed N, Hartskeerl R. Development and validation of a real-time PCR for detection of pathogenic leptospira species in clinical materials. PLoS One. 2009;4(9):e7093.
17. Cerqueira G, McBride A, Picardeau M, Ribeiro S, Moreira A, Morel V, et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. J Med Microbiol. 2009;58(Pt 9):1173-81.
18. Slack A, Symonds M, Dohnt M, Smythe L. Identification of pathogenic Leptospira species by conventional or realtime PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol. 2006;6:95.
19. Gravekamp C, Van de Kemp H, Franzen M, Carrington D, Schoone G, Van Eys G, et al. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J Gen Microbiol. 1993;139(8):1691-700.
20. Ren S, Fu G, Jiang X, Zeng R, Miao Y, Xu H, et al. Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature. 2003;422(6934):888-93.
21. World Organization for Animal Health. Principles of validation of diagnostic assays for infectious diseases. Chapter 1.1.4. In: Manual of diagnostic tests and vaccines for terrestrial animals. Geneva: OIE; 2009.
22. Branger C, Blanchard B, Fillonneau C, Suard I, Aviat F, Chevallier B, et al. Polymerase chain reaction assay specific for pathogenic Leptospira based on the gene hap1 encoding the hemolysis-associated protein-1. FEMS Microbiol Lett. 2005;243(2):437-45.
23. Wangroongsarb P, Saengsongkong W, Petkanjanapong W, Mimgratok M, Panjai D, Wootta W, et al. An application of duplex PCR for detection of Leptospira spp. and Orientia tsutsugamushi from wild rodents. Jpn J Infect Dis. 2008;61(5):407-9.
24. Victoria B, Ahmed A, Zuerner R, Ahmed N, Bulach D, Quinteiro J, et al. Conservation of the S10-spc-alpha locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira. PLoS One. 2008;3(7):e2752.
25. Zuerner R, Hartskeerl R, van de Kemp H, Bal A. Characterization of the Leptospira interrogans S10-spcalpha operon. FEMS Microbiol Lett. 2000;182(2):303-8.
26. Brown P, Gravekamp C, Carrington D, van de Kemp H, Hartskeerl R, Edwards C, et al. Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. J Med Microbiol. 1995;43(2):110-14.
27. Agudelo-Flórez P, Londoño A, Quiroz V, Angel J, Moreno N, Loaiza E, et al. Prevalence of Leptospira spp. in urban rodents from a groceries trade center of Medellin, Colombia. Am J Trop Med Hyg. 2009;81(5):906-10.
28. Szonyi B, Agudelo-Flórez P, Ramírez M, Moreno N, Ko A. An outbreak of severe leptospirosis in capuchin (Cebus) monkeys. Vet J. 2010 May 27 [Epub ahead of print].
29. Nally J, Whitelegge J, Bassilian S, Blanco D, Lovett M. Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Infect Immun. 2007;75(2):766-73.
30. Vital-Brazil J, Balassiano I, Oliveira F, Costa A, Hillen L, Pereira M. Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement. Mem Inst Oswaldo Cruz. 2010;105(3):353-55.
31. Romero E, Billerbeck A, Lando V, Camargo E, Souza C, Yasuda P. Detection of Leptospira DNA in patients with aseptic meningitis by PCR. J Clin Microbiol. 1998;36(5):1453-55.
32. Merien F, Baranton G, Perolat P. Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis. J Infect Dis. 1995;172(1):281-85.