Akt-mediated mechanotransduction in murine fibroblasts during hypertrophic scar formation

Wound Repair and Regeneration

Volumn 19, Issue 1, 2011, Pages 49-58

  Paterno, Josemaria ^a   Vial, Ivan N ^a   Wong, Victor W ^a   Rustad, Kristine C ^a   Sorkin, Michael ^a   Shi, Yubin ^a   Bhatt, Kirit A ^a   Thangarajah, Hariharan ^a   Glotzbach, Jason P ^a   Gurtner, Geoffrey C ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3 KINASE INHIBITOR; PROTEIN KINASE B; WORTMANNIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME INHIBITION; FIBROBLAST; HYPERTROPHIC SCAR; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MECHANOTRANSDUCTION; NONHUMAN; PRIORITY JOURNAL; SCAR FORMATION; SKIN FIBROSIS;

ANDROSTADIENES; ANIMALS; CELL MOVEMENT; CELL SURVIVAL; CICATRIX, HYPERTROPHIC; FIBROBLASTS; MALE; MECHANOTRANSDUCTION, CELLULAR; MICE; MICE, INBRED C57BL; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS; PROTO-ONCOGENE PROTEINS C-AKT; STRESS, MECHANICAL;

EID: 78751552652     PISSN: 10671927     EISSN: 1524475X     Source Type: Journal    
DOI: 10.1111/j.1524-475X.2010.00643.x     Document Type: Article

References (45)

1. Gurtner GC, Werner S, Barrandon Y, Longaker MT., Wound repair and regeneration. Nature 2008; 453: 314-21.
2. Aarabi S, Longaker MT, Gurtner GC., Hypertrophic scar formation following burns and trauma: new approaches to treatment. PLoS Med 2007; 4: e234.
3. Sun KK, Lewis WH., Hypertrophic scar: a genetic hypothesis. Burns 1990; 16: 176-8.
4. Tomasek JJ, Petro JA, Eddy RJ., Evidence for the nonmuscle nature of the "myofibroblast" of granulation tissue and hypertropic scar. An immunofluorescence study. Am J Pathol 1988; 130: 252-60.
5. Aarabi S, Bhatt KA, Shi Y, Paterno J, Chang EI, Loh SA, Holmes JW, Longaker MT, Yee H, Gurtner GC., Mechanical load initiates hypertrophic scar formation through decreased cellular apoptosis. FASEB J 2007; 21: 3250-61. (Pubitemid 47565543)
6. Abrams JM, Carlson MA, Zhu M, Grinnell F., Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue. Exp Cell Res 1999; 248: 608-19.
7. Nho RS, Xia H, Kahm J, Kleidon J, Diebold D, Henke CA., Role of integrin-linked kinase in regulating phosphorylation of Akt and fibroblast survival in type I collagen matrices through a beta1 integrin viability signaling pathway. J Biol Chem 2005; 280: 26630-9.
8. Yano Y, Geibel J, Sumpio BE., Cyclic strain induces reorganization of integrin alpha 5 beta 1 and alpha 2 beta 1 in human umbilical vein endothelial cells. J Cell Biochem 1997; 64: 505-13.
9. Derderian CA, Bastidas N, Lerman OZ, Bhatt KA, Lin SE, Voss J, Holmes JW, Levine JP, Gurtner GC., Mechanical strain alters gene expression in an in vitro model of hypertrophic scarring. Ann Plast Surg 2005; 55: 69-75.
10. Kakinuma N, Roy BC, Zhu Y, Wang Y, Kiyama R., Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling. J Cell Biol 2008; 181: 537-49.
11. Bujor AM, Pannu J, Bu S, Smith EA, Muise-Helmericks RC, Trojanowska M., Akt blockade downregulates collagen and upregulates MMP1 in human dermal fibroblasts. J Invest Dermatol 2008; 128: 1906-14.
12. Krepinsky JC, Li Y, Chang Y, Liu L, Peng F, Wu D, Tang D, Scholey J, Ingram AJ., Akt mediates mechanical strain-induced collagen production by mesangial cells. J Am Soc Nephrol 2005; 16: 1661-72.
13. Hinz B, Celetta G, Tomasek JJ, Gabbiani G, Chaponnier C., Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol Biol Cell 2001; 12: 2730-41.
14. Beningo KA, Dembo M, Wang YL., Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors. Proc Natl Acad Sci USA 2004; 101: 18024-9.
15. Bellin RM, Kubicek JD, Frigault MJ, Kamien AJ, Steward RL Jr., Barnes HM, Digiacomo MB, Duncan LJ, Edgerly CK, Morse EM, Park CY, Fredberg JJ, Cheng CM, LeDuc PR., Defining the role of syndecan-4 in mechanotransduction using surface-modification approaches. Proc Natl Acad Sci USA 2009; 106: 22102-7.
16. Danciu TE, Gagari E, Adam RM, Damoulis PD, Freeman MR., Mechanical strain delivers anti-apoptotic and proliferative signals to gingival fibroblasts. J of Dental Res 2004; 83: 596-601.
17. Lee AA, Delhaas T, McCulloch AD, Villarreal FJ., Differential responses of adult cardiac fibroblasts to in vitro biaxial strain patterns. J Mol Cell Cardiol 1999; 31: 1833-43.
18. Barker SA, Caldwell KK, Hall A, Martinez AM, Pfeiffer JR, Oliver JM, Wilson BS., Wortmannin blocks lipid and protein kinase activities associated with PI 3-kinase and inhibits a subset of responses induced by Fc epsilon R1 cross-linking. Mol Biol Cell 1995; 6: 1145-58.
19. Liang C-C, Park AY, Guan J-L., In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007; 2: 329-33.
20. Jiang Q, Zhou C, Bi Z, Wan Y., EGF-induced cell migration is mediated by ERK and PI3K/AKT pathways in cultured human lens epithelial cells. J Ocul Pharmacol Ther 2006; 22: 93-102.
21. Reiske HR, Kao S-C, Cary LA, Guan J-L, Lai J-F, Chen H-C., Requirement of phosphatidylinositol 3-kinase in focal adhesion kinase-promoted cell migration. J Biol Chem 1999; 274: 12361-6.
22. Atance J, Yost MJ, Carver W., Influence of the extracellular matrix on the regulation of cardiac fibroblast behavior by mechanical stretch. Journal of Cellular Physiology 2004; 200: 377-86.
23. Yang G, Crawford RC, Wang JHC., Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions. J Biomech 2004; 37: 1543-50.
24. Zeichen J, van Griensven M, Bosch U., The proliferative response of isolated human tendon fibroblasts to cyclic biaxial mechanical strain. Am J Sports Med 2000; 28: 888-92.
25. Junker JP, Kratz C, Tollback A, Kratz G., Mechanical tension stimulates the transdifferentiation of fibroblasts into myofibroblasts in human burn scars. Burns 2008; 34: 942-6.
26. Lee JYY, Yang CC, Chao SC, Wong TW., Histopathological differential diagnosis of keloid and hypertrophic scar. Amer J Dermatopathol 2004; 26: 379-84.
27. Sheridan RL., Burn care: results of technical and organizational progress. JAMA 2003; 290: 719-22.
28. Gieni RS, Hendzel MJ., Mechanotransduction from the ECM to the genome: are the pieces now in place? J Cell Biochem 2008; 104: 1964-87.
29. Orr AW, Helmke BP, Blackman BR, Schwartz MA., Mechanisms of mechanotransduction. Dev Cell 2006; 10: 11-20.
30. Onishi K, Higuchi M, Asakura T, Masuyama N, Gotoh Y., The PI3K-Akt pathway promotes microtubule stabilization in migrating fibroblasts. Genes Cells 2007; 12: 535-46.
31. Ingber DE, Tensegrity I., Cell structure and hierarchical systems biology. J Cell Sci 2003; 116: 1157-73.
32. Scaffidi AK, Moodley YP, Weichselbaum M, Thompson PJ, Knight DA., Regulation of human lung fibroblast phenotype and function by vitronectin and vitronectin integrins. J Cell Sci 2001; 114: 3507-16.
33. Shi-Wen X, Chen Y, Denton CP, Eastwood M, Renzoni EA, Bou-Gharios G, Pearson JD, Dashwood M, du Bois RM, Black CM, Leask A, Abraham DJ., Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/Akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts. Mol Biol Cell 2004; 15: 2707-19.
34. Ding Q, Gladson CL, Wu H, Hayasaka H, Olman MA., Focal adhesion kinase (FAK)-related non-kinase inhibits myofibroblast differentiation through differential MAPK activation in a FAK-dependent manner. J Biol Chem 2008; 283: 26839-49.
35. Desmouliere A, Chaponnier C, Gabbiani G., Tissue repair, contraction, and the myofibroblast. Wound Rep Regen 2005; 13: 7-12.
36. Hinz B, Phan SH, Thannickal VJ, Galli A, Bochaton-Piallat ML, Gabbiani G., The myofibroblast: one function, multiple origins. Am J Pathol 2007; 170: 1807-16.
37. Hinz B., Formation and function of the myofibroblast during tissue repair. J Invest Dermatol 2007; 127: 526-37.
38. Celil Aydemir AB, Lee S, Won Kim D, Gardner TR, Prince D, Mok Ahn J, Young-In Lee F., Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction. Ann NY Acad Sci 2007; 1117: 138-42.
39. Martin DK, Bootcov MR, Campbell TJ, French PW, Breit SN., Human macrophages contain a stretch-sensitive potassium channel that is activated by adherence and cytokines. J Membr Biol 1995; 147: 305-15.
40. Delehedde M, Sergeant N, Lyon M, Rudland PS, Fernig DG., Hepatocyte growth factor/scatter factor stimulates migration of rat mammary fibroblasts through both mitogen-activated protein kinase and phosphatidylinositol 3-kinase/Akt pathways. Eur J Biochem 2001; 268: 4423-9.
41. Horowitz J, Rogers D, Sharma V, Vittal R, White E, Cui Z, Thannickal VJ., Combinatorial activation of FAK and AKT by transforming growth factor-beta1 confers an anoikis-resistant phenotype to myofibroblasts. Cell Signal 2007; 19: 761-71.
42. Xia H, Nho RS, Kahm J, Kleidon J, Henke CA., Focal adhesion kinase is upstream of phosphatidylinositol 3-kinase/Akt in regulating fibroblast survival in response to contraction of type I collagen matrices via a beta 1 integrin viability signaling pathway. J Biol Chem 2004; 279: 33024-34.
43. Vittal R, Horowitz JC, Moore BB, Zhang H, Martinez FJ, Toews GB, Standiford TJ, Thannickal VJ., Modulation of Prosurvival signaling in fibroblasts by a protein kinase inhibitor protects against fibrotic tissue injury. Am J Pathol 2005; 166: 367-75.
44. Chaturvedi LS, Marsh HM, Basson MD., Src and focal adhesion kinase mediate mechanical strain-induced proliferation and ERK1/2 phosphorylation in human H441 pulmonary epithelial cells. Am J Physiol Cell Physiol 2007; 292: C1701-13.
45. Hayashida T, Wu MH, Pierce A, Poncelet AC, Varga J, Schnaper HW., MAP-kinase activity necessary for TGFbeta1-stimulated mesangial cell type I collagen expression requires adhesion-dependent phosphorylation of FAK tyrosine 397. J Cell Sci 2007; 120 (Pt 23): 4230-40.