Screening complex effluents for estrogenic activity with the T47D-KBluc cell bioassay: Assay optimization and comparison with in vivo responses in fish

Environmental Toxicology and Chemistry

Volumn 30, Issue 2, 2011, Pages 439-445

  Wehmas, Leah C ^a   Cavallin, Jenna E ^a   Durhan, Elizabeth J ^a   Kahl, Michael D ^a   Martinovic, Dalma ^a,b   Mayasich, Joe ^c   Tuominen, Tim ^c   Villeneuve, Daniel L ^a   Ankley, Gerald T ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b UNIVERSITY OF ST THOMAS   (United States)

^c Western Lake Superior Sanitary District   (United States)

Author keywords

Complex mixtures; Estrogens; Gene expression; Solid phase extraction

Indexed keywords

ASSAY OPTIMIZATION; CELL BIOASSAY; COMPLEX MIXTURE; ESTROGEN RECEPTOR; ESTROGENIC ACTIVITIES; ESTROGENIC CHEMICALS; ESTROGENICITY; ESTROGENS; FATHEAD MINNOW; IN-VITRO; IN-VIVO; PIMEPHALES PROMELAS; PREPARATION METHOD; REGULATED GENES; SAMPLE PREPARATION METHODS; SOLID-PHASE EXTRACTION; VITELLOGENIN; WASTEWATER EFFLUENTS; WASTEWATER TREATMENT PLANTS; WWTP EFFLUENT;

BIOASSAY; EXTRACTION; GENES; TRANSCRIPTION; WASTEWATER; WASTEWATER TREATMENT; WATER TREATMENT PLANTS;

EFFLUENTS;

ESTRADIOL; ESTROGEN RECEPTOR; ESTROGEN RECEPTOR ALPHA; VITELLOGENIN;

BIOASSAY; COMPARATIVE STUDY; CYPRINID; EFFLUENT; ENDOCRINE DISRUPTOR; ESTROGENIC COMPOUND; EXTRACTION METHOD; FISH; GENE EXPRESSION; OPTIMIZATION; POLLUTION EXPOSURE; SAMPLE PREPARATION; WASTEWATER; WATER TREATMENT;

ARTICLE; EFFLUENT; ESTROGEN ACTIVITY; FISH; GENETIC TRANSCRIPTION; IN VITRO STUDY; IN VIVO STUDY; MALE; NONHUMAN; PIMEPHALES PROMELAS; PRIORITY JOURNAL; WASTE WATER TREATMENT PLANT;

ANIMALS; CELL LINE; CYPRINIDAE; ESTRADIOL; ESTROGENS; HUMANS; LIVER; MALE; VITELLOGENINS; WASTE DISPOSAL, FLUID; WATER POLLUTANTS, CHEMICAL;

PIMEPHALES PROMELAS;

EID: 78651354453     PISSN: 07307268     EISSN: 15528618     Source Type: Journal    
DOI: 10.1002/etc.388     Document Type: Article

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