The role of carbohydrate-binding module (CBM) repeat of a multimodular xylanase (XynX) from Clostridium thermocellum in cellulose and xylan binding

Journal of Microbiology

Volumn 48, Issue 6, 2010, Pages 856-861

  Selvaraj, Thangaswamy ^a   Kim, Sung Kyum ^a   Kim, Yong Ho ^a   Jeong, Yu Seok ^a   Kim, Yu Jeong ^a   Phuong, Nguyen Dinh ^a   Jung, Kyung Hwa ^b   Kim, Jungho ^a   Yun, Han Dae ^c   Kim, Hoon ^a  

^a Sunchon National University   (South Korea)

^b Amicogen Inc   (South Korea)

^c Gyeongsang National University   (South Korea)

Author keywords

carbohydrate binding module; cellulose and xylan binding; Clostridium thermocellum XynX; gel elution; truncated xylanases

Indexed keywords

CELLULOSE; GLYCOSIDASE; RECOMBINANT PROTEIN; XYLAN;

ARTICLE; BINDING SITE; CLOSTRIDIUM THERMOCELLUM; ENZYMOLOGY; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN BINDING;

BINDING SITES; CELLULOSE; CLONING, MOLECULAR; CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSIDE HYDROLASES; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE DELETION; XYLANS;

CLOSTRIDIUM THERMOCELLUM;

EID: 78651289576     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: 10.1007/s12275-010-0285-5     Document Type: Article

References (26)

1. Bayer, E. A., J-P. Belaich, Y. Shoham, and R. Lamed. 2004. The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Annu. Rev. Microbiol. 58, 521-554.
2. Bayer, E. A., R. Lamed, B. A. White, and H. J. Flint. 2008. From cellulosomes to cellulosomics. Chem. Rec. 8, 364-377.
3. Bayer, E. A., Y. Shoham, and R. Lamed. 2006. Cellulose-decomposing bacteria and their enzyme systems. Prokaryotes 2, 578-617.
4. Beguin, P. and M. Lemaire. 1996. The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit. Rev. Biochem. Mol. Biol. 31, 201-236.
5. Boraston, A. B., A. L. Creagh, M. M. Alam, J. M. Kormos, P. Tomme, C. A. Haynes, R. A. J. Warren, and D. G. Kilburn. 2001. Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A. Biochemistry 40, 6240-6247.
6. Charnock, S. J., D. N. Bolam, J. P. Turkenburg, H. J. Gilbert, L. M. A. Ferreira, G. J. Davis, and C. M. G. A. Fontes. 2000. The X6 "thermostabilizing" domains of xylanases are carbohydratebinding modules: structure and biochemistry of the Clostridium thermocellum X6b domain. Biochemistry 39, 5013-5021.
7. Demain, A. L., M. Newcomb, and J. H. Wu. 2005. Cellulase, clostridia, and ethanol. Microbiol. Mol. Biol. Rev. 69, 124-154.
8. Dias, F. M. V., A. Goyal, H. J. Gilbert, J. A. M. Prates, L. M. A. Ferreira, and C. M. G. A. Fontes. 2004. The N-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum is not a thermostabilizing domain. FEMS Microbiol. Lett. 238, 71-78.
9. Fernandes, A. C., C. M. G. A. Fontes, H. J. Gilbert, G. P. Hazelwood, T. H. Fernandes, and L. M. A. Ferreira. 1999. Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase modules and the presence of xylanbinding domains in the enzyme complexes. Biochem. J. 342, 105-110.
10. Fontes, C. M. G. A., G. P. Hazlewood, E. Morag, J. Hall, B. H. Hirst, and H. J. Gilbert. 1995. Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria. Biochem. J. 307, 151-158.
11. Gold, N. D. and V. J. J. Martin. 2007. Global view of the clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J. Bacteriol. 189, 6786-6795.
12. Grepinet, O., M. C. Chebrou, and P. Beguin. 1988. Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. J. Bacteriol. 170, 4582-4588.
13. Hall, J. and H. J. Gilbert. 1988. The nucleotide sequence of a carboxymethyl-cellulase gene from Pseudomonas fluorescens subsp. cellulosa. Mol. Gen. Genet. 213, 112-117.
14. Hayashi, H., K. I. Takagi, M. Fukumura, T. Kimura, S. Karita, K. Sakka, and K. Ohmiya. 1997. Sequence of Xyn C and properties of XynC, a major component of the Clostridium thermocellum cellulosome. J. Bacteriol. 179, 4246-4253.
15. Hayashi, H., M. Takehara, T. Hattori, T. Kimura, S. Karita, K. Sakka, and K. Ohmiya. 1999. Nucleotide sequences of two contiguous and highly homologous xylanase genes XynA and XynB and characterization of XynA from Clostridium thermocellum. Appl. Microbiol. Biotechnol. 51, 348-357.
16. Jung, K. H., K. M. Lee, H. Kim, K. H. Yoon, S. H. Park, and M. Y. Pack. 1998. Cloning and expression of a Clostridium thermocellum xylanase gene in Escherichia coli. Biochem. Mol. Biol. Int. 44, 283-292.
17. Kim, H., K. H. Jung, and M. Y. Pack. 2000. Molecular characterization of xynX, a gene encoding a multidomain xylanase with a thermostabilizing domain from Clostridium thermocellum. Appl. Microbiol. Biotechnol. 54, 521-527.
18. Kim, H., S. F. Kim, D. H. Ahn, J. H. Lee, and M. Y. Pack. 1995. Internal cleavage of Bacillus subtilis BSE616 endo-β-1, 4-glucanase expressed in Escherichia coli. J. Microbiol. Biotechnol. 5, 26-30.
19. Kim, H., S. F. Kim, and M. Y. Pack. 1991. C-Terminal processing of Bacillus subtilis BSE 616 endo-β-1, 4-glucanase in Bacillus megaterium. Biotechnol. Lett. 13, 799-804.
20. Lowry, O. H., N. J. Rosenbrough, A. L. Farr, and R. L. Randell. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
21. Marchler-Bauer, A., J. B. Anderson, F. Chitsaz, M. K. Derbyshire, C. DeWeese-Scott, J. H. Fong, L. Y. Geer, and et al. 2009. CDD: specific functional annotation with the Conserved Domain Database. Nucleic Acids. Res. 37(Database issue), 205-210.
22. Meissner, K., D. Wassenberg, and W. Liebl. 2000. The thermostabilizing domain of the modular xylanase XynA of Thermotoga maritima represents a novel type of binding domain with affinity for soluble xylan and mixed-linkage β-1, 3/β-1, 4-glucan. Mol. Microbiol. 36, 898-912.
23. Sunna, A., M. D. Gibbs, and P. L. Bergquist. 2000. The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain. Biochem. J. 346, 583-586.
24. Shin, E. S., M. J. Yang, K. H. Jung, E. J. Kwon, J. S. Jung, S. K. Park, J. Kim, H. D. Yun, and H. Kim. 2002. Influence of the transposition of the thermostabilizing domain of Clostridium thermocellum xylanase (XynX) on xylan binding and thermostabilization. Appl. Environ. Microbiol. 68, 3496-3501.
25. Zhao, G., E. Ali, R. Araki, M. Sakka, T. Kimura, and K. Sakka. 2005. Function of the family-9 and family-22 carbohydrate-binding modules in a modular β-1, 3-1, 4-glucanase/xylanase derived from Clostridium stercorarium Xyn10B. Biosci. Biotechnol. Biochem. 69, 1562-1567.
26. Zverlov, V. V., N. Schantz, P. Schmitt-Kpplin, and W. H. Schwarz. 2005. Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucanase Xgh74A and endoxylanase Xyn10D. Microbiology 151, 3395-3401.