메뉴 건너뛰기




Volumn 35, Issue 4, 2010, Pages 525-531

Demonstration of a visual cell-based assay for screening glucose transporter 4 translocation modulators in real time

Author keywords

CHO cells; eGFP; GLUT4; live cell imaging; natural product; qualitative assay; translocation

Indexed keywords

GLUCOSE TRANSPORTER 4;

EID: 78651265126     PISSN: 02505991     EISSN: 02505991     Source Type: Journal    
DOI: 10.1007/s12038-010-0060-0     Document Type: Article
Times cited : (22)

References (26)
  • 1
    • 72049127269 scopus 로고    scopus 로고
    • A robust assay measuring GLUT4 translocation in rat myoblasts overexpressing GLUT4-myc and AS160-v2
    • 1:CAS:528:DC%2BD1MXhsFyitb%2FP 10.1016/j.ab.2009.10.036 19854150
    • D. Baus Y. Yan Z. Li T. Garyantes M. de Hoop N. Tennagels 2010 A robust assay measuring GLUT4 translocation in rat myoblasts overexpressing GLUT4-myc and AS160-v2 Anal. Biochem. 397 233 240 1:CAS:528:DC%2BD1MXhsFyitb%2FP 10.1016/j.ab.2009.10.036 19854150
    • (2010) Anal. Biochem. , vol.397 , pp. 233-240
    • Baus, D.1    Yan, Y.2    Li, Z.3    Garyantes, T.4    De Hoop, M.5    Tennagels, N.6
  • 2
    • 0037180775 scopus 로고    scopus 로고
    • Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c
    • DOI 10.1038/nature01246
    • A. Bose A. Guilherme S.I. Robida S. M. Nicoloro Q. L. Zhou Z. Y. Jiang D. P. Pomerleau M. P. Czech 2002 Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c Nature (London) 420 821 824 1:CAS:528:DC%2BD38XpsFygt7c%3D 10.1038/nature01246 (Pubitemid 36019630)
    • (2002) Nature , vol.420 , Issue.6917 , pp. 821-824
    • Bose, A.1    Guilherme, A.2    Robida, S.I.3    Nicoloro, S.M.C.4    Zhou, Q.L.5    Jiang, Z.Y.6    Pomerleau, D.P.7    Czech, M.P.8
  • 3
    • 0036270751 scopus 로고    scopus 로고
    • Regulated transport of the glucose transporter GLUT4
    • DOI 10.1038/nrm782
    • N. J. Bryant R. Govers D. E. James 2002 Regulated transport of the glucose transporter GLUT4 Nat. Rev. Mol. Cell Biol. 3 267 277 1:CAS:528:DC%2BD38XjslOnuro%3D 10.1038/nrm782 11994746 (Pubitemid 34619262)
    • (2002) Nature Reviews Molecular Cell Biology , vol.3 , Issue.4 , pp. 267-277
    • Bryant, N.J.1    Govers, R.2    James, D.E.3
  • 4
    • 33845797151 scopus 로고    scopus 로고
    • TIRFing out Studies on Glut4 Trafficking
    • DOI 10.1016/j.devcel.2006.12.008, PII S1534580706005715
    • X. W. Chen A. R. Saltiel 2007 TIRFing out studies on Glut4 trafficking Dev. Cell 12 4 5 1:CAS:528:DC%2BD2sXpt1WjtA%3D%3D 10.1016/j.devcel.2006.12.008 17199036 (Pubitemid 46002605)
    • (2007) Developmental Cell , vol.12 , Issue.1 , pp. 4-5
    • Chen, X.-W.1    Saltiel, A.R.2
  • 5
    • 0035929258 scopus 로고    scopus 로고
    • Insulin-regulated trafficking of dual-labeled glucose transporter 4 in primary rat adipose cells
    • DOI 10.1006/bbrc.2001.5620
    • K. Dawson A. Aviles-Hernandez S. W. Cushman D. Malide 2001 Insulin-regulated trafficking of dual-labeled glucose transporter 4 in primary rat adipose cells Biochem. Biophys. Res. Commun. 287 445 454 1:CAS:528:DC%2BD3MXms12qs70%3D 10.1006/bbrc.2001.5620 11554749 (Pubitemid 32917619)
    • (2001) Biochemical and Biophysical Research Communications , vol.287 , Issue.2 , pp. 445-454
    • Dawson, K.1    Aviles-Hernandez, A.2    Cushman, S.W.3    Malide, D.4
  • 6
    • 0030590477 scopus 로고    scopus 로고
    • Dynamics of insulin-stimulated translocation of GLUT4 in single living cells visualised using green fluorescent protein
    • DOI 10.1016/0014-5793(96)00879-4
    • S. P. Dobson C. Livingstone G. W. Gould J. M. Tavare 1996 Dynamics of insulin-stimulated translocation of GLUT4 in single living cells visualised using green fluorescent protein FEBS Lett. 393 179 184 1:CAS:528: DyaK28Xls1Kmurg%3D 10.1016/0014-5793(96)00879-4 8814285 (Pubitemid 26298091)
    • (1996) FEBS Letters , vol.393 , Issue.2-3 , pp. 179-184
    • Dobson, S.P.1    Livingstone, C.2    Gould, G.W.3    Tavare, J.M.4
  • 7
    • 0034624008 scopus 로고    scopus 로고
    • Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes
    • DOI 10.1074/jbc.M003432200
    • A. Guilherme M. Emoto J. M. Buxton S. Bose R. Sabini W. E. Theurkauf J. Leszyk M. P. Czech 2000 Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes J. Biol. Chem. 275 38151 38159 1:CAS:528:DC%2BD3cXoslymsro%3D 10.1074/jbc.M003432200 10950952 (Pubitemid 32009132)
    • (2000) Journal of Biological Chemistry , vol.275 , Issue.49 , pp. 38151-38159
    • Guilherme, A.1    Emoto, M.2    Buxton, J.M.3    Bose, S.4    Sabini, R.5    Theurkauf, W.E.6    Leszyk, J.7    Czech, M.P.8
  • 8
    • 0033609911 scopus 로고    scopus 로고
    • Identification of wortmannin-sensitive targets in 3T3-L1 adipocytes. Dissociation of insulin-stimulated glucose uptake and GLUT4 translocation
    • DOI 10.1074/jbc.274.35.24677
    • S. F. Hausdorff D. C. Fingar K. Morioka L. A. Garza E. L. Whiteman S. A. Summers M. J. Birnbaum 1999 Identification of wortmannin-sensitive targets in 3T3-L1 adipocytes. Dissociation of insulin-stimulated glucose uptake and glut4 translocation J. Biol. Chem. 274 24677 24684 1:CAS:528:DyaK1MXls1yntb4%3D 10.1074/jbc.274.35.24677 10455135 (Pubitemid 29404667)
    • (1999) Journal of Biological Chemistry , vol.274 , Issue.35 , pp. 24677-24684
    • Hausdorff, S.F.1    Fingar, D.C.2    Morioka, K.3    Garza, L.A.4    Whiteman, E.L.5    Summers, S.A.6    Birnbaum, M.J.7
  • 9
    • 0037016725 scopus 로고    scopus 로고
    • A phosphatidylinositol 3-kinase-independent insulin signaling pathway to N-WASP/Arp2/3/F-actin required for GLUT4 glucose transporter recycling
    • DOI 10.1074/jbc.M108280200
    • Z. Y. Jiang A. Chawla A. Bose M. Way M. P. Czech 2002 A phosphatidylinositol 3-kinase-independent insulin signaling pathway to N-WASP/Arp2/3/F-actin required for GLUT4 glucose transporter recycling J. Biol. Chem. 277 509 515 1:CAS:528:DC%2BD38XjslGgug%3D%3D 10.1074/jbc.M108280200 11694514 (Pubitemid 34952086)
    • (2002) Journal of Biological Chemistry , vol.277 , Issue.1 , pp. 509-515
    • Jiang, Z.Y.1    Chawla, A.2    Bose, A.3    Way, M.4    Czech, M.P.5
  • 10
    • 33845881411 scopus 로고    scopus 로고
    • Mechanisms linking obesity to insulin resistance and type 2 diabetes
    • DOI 10.1038/nature05482, PII NATURE05482
    • S. E. Kahn R. L. Hull K. M. Utzschneider 2006 Mechanisms linking obesity to insulin resistance and type 2 diabetes Nature (London) 444 840 846 1:CAS:528:DC%2BD28XhtlShtrzP 10.1038/nature05482 (Pubitemid 46024990)
    • (2006) Nature , vol.444 , Issue.7121 , pp. 840-846
    • Kahn, S.E.1    Hull, R.L.2    Utzschneider, K.M.3
  • 11
    • 0027210896 scopus 로고
    • Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a c-myc epitope into an exofacial GLUT4 domain
    • 1:CAS:528:DyaK3sXkvVKrs7s%3D 7686158
    • F. Kanai Y. Nishioka H. Hayashi S. Kamohara M. Todaka Y. Ebina 1993 Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a c-myc epitope into an exofacial GLUT4 domain J. Biol. Chem. 268 14523 14526 1:CAS:528:DyaK3sXkvVKrs7s%3D 7686158
    • (1993) J. Biol. Chem. , vol.268 , pp. 14523-14526
    • Kanai, F.1    Nishioka, Y.2    Hayashi, H.3    Kamohara, S.4    Todaka, M.5    Ebina, Y.6
  • 12
    • 0025999031 scopus 로고
    • Chronic treatment with insulin selectively down-regulates cell-surface GLUT4 glucose transporters in 3T3-L1 adipocytes
    • 1:CAS:528:DyaK3MXksVagu7o%3D 2050674
    • I. J. Kozka A. E. Clark G. D. Holman 1991 Chronic treatment with insulin selectively down-regulates cell-surface GLUT4 glucose transporters in 3T3-L1 adipocytes J. Biol. Chem. 266 11726 11731 1:CAS:528:DyaK3MXksVagu7o%3D 2050674
    • (1991) J. Biol. Chem. , vol.266 , pp. 11726-11731
    • Kozka, I.J.1    Clark, A.E.2    Holman, G.D.3
  • 13
    • 0344993515 scopus 로고
    • Adipose conversion of 3T3 cells depends on a serum factor
    • 1:CAS:528:DyaE1MXhtVWisbg%3D 10.1073/pnas.75.12.6107 282628
    • W. Kuri-Harcuch H. Green 1978 Adipose conversion of 3T3 cells depends on a serum factor Proc. Natl. Acad. Sci. USA 75 6107 6109 1:CAS:528: DyaE1MXhtVWisbg%3D 10.1073/pnas.75.12.6107 282628
    • (1978) Proc. Natl. Acad. Sci. USA , vol.75 , pp. 6107-6109
    • Kuri-Harcuch, W.1    Green, H.2
  • 14
    • 0035196362 scopus 로고    scopus 로고
    • Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles
    • 1:CAS:528:DC%2BD3MXovFOms7s%3D 11694583
    • M. A. Lampson J. Schmoranzer A. Zeigerer S. M. Simon T. E. McGraw 2001 Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles Mol. Biol. Cell 12 3489 3501 1:CAS:528:DC%2BD3MXovFOms7s%3D 11694583
    • (2001) Mol. Biol. Cell , vol.12 , pp. 3489-3501
    • Lampson, M.A.1    Schmoranzer, J.2    Zeigerer, A.3    Simon, S.M.4    McGraw, T.E.5
  • 15
    • 0026509981 scopus 로고
    • GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport
    • 1:CAS:528:DyaK38XisFejsrY%3D 10.1073/pnas.89.8.3493 1314390
    • J. C. Lawrence Jr R. C. Piper L. J. Robinson D. E. James 1992 GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport Proc. Natl. Acad. Sci. USA 89 3493 3497 1:CAS:528:DyaK38XisFejsrY%3D 10.1073/pnas.89.8.3493 1314390
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 3493-3497
    • Lawrence Jr., J.C.1    Piper, R.C.2    Robinson, L.J.3    James, D.E.4
  • 16
    • 61449130618 scopus 로고    scopus 로고
    • Development of a novel GLUT4 translocation assay for identifying potential novel therapeutic targets for insulin sensitization
    • 1:CAS:528:DC%2BD1MXhs1Onsrk%3D 10.1042/BJ20082051 19035854
    • F. Liu Q. Dallas-Yang G. Castriota P. Fischer F. Santini M. Ferrer J. Li T. E. Akiyama J. P. Berger B. B. Zhang G. Jiang 2009 Development of a novel GLUT4 translocation assay for identifying potential novel therapeutic targets for insulin sensitization Biochem. J. 418 413 420 1:CAS:528:DC%2BD1MXhs1Onsrk%3D 10.1042/BJ20082051 19035854
    • (2009) Biochem. J. , vol.418 , pp. 413-420
    • Liu, F.1    Dallas-Yang, Q.2    Castriota, G.3    Fischer, P.4    Santini, F.5    Ferrer, M.6    Li, J.7    Akiyama, T.E.8    Berger, J.P.9    Zhang, B.B.10    Jiang, G.11
  • 17
    • 33846868667 scopus 로고    scopus 로고
    • Variability in 3T3-L1 adipocyte differentiation depending on cell culture dish
    • DOI 10.1016/j.ab.2006.12.016, PII S0003269706009110
    • A. Mehra I. Macdonald T. S. Pillay 2007 Variability in 3T3-L1 adipocyte differentiation depending on cell culture dish Anal. Biochem. 362 281 283 1:CAS:528:DC%2BD2sXhvVynurw%3D 10.1016/j.ab.2006.12.016 17241610 (Pubitemid 46227315)
    • (2007) Analytical Biochemistry , vol.362 , Issue.2 , pp. 281-283
    • Mehra, A.1    Macdonald, I.2    Pillay, T.S.3
  • 18
    • 0030659755 scopus 로고    scopus 로고
    • GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein
    • 1:CAS:528:DyaK2sXns1ams74%3D 9581537
    • P. B. Oatey D. H. Van Weering S. P. Dobson G. W. Gould J. M. Tavare 1997 GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein Biochem. J. 327 637 642 1:CAS:528:DyaK2sXns1ams74%3D 9581537
    • (1997) Biochem. J. , vol.327 , pp. 637-642
    • Oatey, P.B.1    Van Weering, D.H.2    Dobson, S.P.3    Gould, G.W.4    Tavare, J.M.5
  • 19
    • 0030933164 scopus 로고    scopus 로고
    • Overexpression and activation of the insulin receptor enhances expression of ERCC-1 messenger ribonucleic acid in cultured cells
    • 1:CAS:528:DyaK2sXislCjtLc%3D 10.1210/en.138.5.1829 9112375
    • R. Perfetti W. Lee-Kwon C. Montrose-Rafizadeh M. Bernier 1997 Overexpression and activation of the insulin receptor enhances expression of ERCC-1 messenger ribonucleic acid in cultured cells Endocrinology 138 1829 1835 1:CAS:528:DyaK2sXislCjtLc%3D 10.1210/en.138.5.1829 9112375
    • (1997) Endocrinology , vol.138 , pp. 1829-1835
    • Perfetti, R.1    Lee-Kwon, W.2    Montrose-Rafizadeh, C.3    Bernier, M.4
  • 20
    • 0033913213 scopus 로고    scopus 로고
    • Signaling pathways in insulin action: Molecular targets of insulin resistance
    • 1:CAS:528:DC%2BD3cXkvFWhu7g%3D 10.1172/JCI10582 10903329
    • J. E. Pessin A. R. Saltiel 2000 Signaling pathways in insulin action: molecular targets of insulin resistance J. Clin. Invest. 106 165 169 1:CAS:528:DC%2BD3cXkvFWhu7g%3D 10.1172/JCI10582 10903329
    • (2000) J. Clin. Invest. , vol.106 , pp. 165-169
    • Pessin, J.E.1    Saltiel, A.R.2
  • 22
    • 0025951084 scopus 로고
    • Immunoelectron microscopic demonstration of insulinstimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4
    • 1:CAS:528:DyaK3MXlt1Ortr0%3D 10.1073/pnas.88.15.6893 1713695
    • R. M. Smith M. J. Charron N. Shah H. F. Lodish L. Jarett 1991 Immunoelectron microscopic demonstration of insulinstimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4 Proc. Natl. Acad. Sci. USA 88 6893 6897 1:CAS:528:DyaK3MXlt1Ortr0%3D 10.1073/pnas.88.15.6893 1713695
    • (1991) Proc. Natl. Acad. Sci. USA , vol.88 , pp. 6893-6897
    • Smith, R.M.1    Charron, M.J.2    Shah, N.3    Lodish, H.F.4    Jarett, L.5
  • 23
    • 0027279805 scopus 로고
    • Genistein inhibits insulin-stimulated glucose transport and decreases immunocytochemical labeling of GLUT4 carboxyl-terminus without affecting translocation of GLUT4 in isolated rat adipocytes: Additional evidence of GLUT4 activation by insulin
    • DOI 10.1006/abbi.1993.1033
    • R. M. Smith J. J. Tiesinga N. Shah J. A. Smith L. Jarett 1993 Genistein inhibits insulin-stimulated glucose transport and decreases immunocytochemical labeling of GLUT4 carboxyl-terminus without affecting translocation of GLUT4 in isolated rat adipocytes: additional evidence of GLUT4 activation by insulin Arch. Biochem. Biophys. 300 238 246 1:CAS:528:DyaK3sXntl2qtQ%3D%3D 10.1006/abbi.1993.1033 8424658 (Pubitemid 23225742)
    • (1993) Archives of Biochemistry and Biophysics , vol.300 , Issue.1 , pp. 238-246
    • Smith, R.M.1    Tiesinga, J.J.2    Shah, N.3    Smith, J.A.4    Jarett, L.5
  • 24
    • 0008385878 scopus 로고
    • Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site
    • DOI 10.1073/pnas.77.5.2542
    • K. Suzuki T. Kono 1980 Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site Proc. Natl. Acad. Sci. USA 77 2542 2545 1:CAS:528:DyaL3cXktlOkt7c%3D 10.1073/pnas.77.5.2542 6771756 (Pubitemid 10049817)
    • (1980) Proceedings of the National Academy of Sciences of the United States of America , vol.77 , Issue.5 , pp. 2542-2545
    • Suzuki, K.1    Kono, T.2
  • 25
    • 30644477939 scopus 로고    scopus 로고
    • The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin signalling pathway
    • DOI 10.1038/sj.bjp.0706312
    • M. V. Vijayakumar S. Singh R. R. Chhipa M. K. Bhat 2005 The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin signalling pathway Br. J. Pharmacol. 146 41 48 1:CAS:528: DC%2BD2MXpslaltbY%3D 10.1038/sj.bjp.0706312 15980869 (Pubitemid 43086093)
    • (2005) British Journal of Pharmacology , vol.146 , Issue.1 , pp. 41-48
    • Vijayakumar, M.V.1    Singh, S.2    Chhipa, R.R.3    Bhat, M.K.4
  • 26
    • 0032496396 scopus 로고    scopus 로고
    • GLUT4 translocation by insulin in intact muscle cells: Detection by a fast and quantitative assay
    • DOI 10.1016/S0014-5793(98)00423-2, PII S0014579398004232
    • Q. Wang Z. Khayat K. Kishi Y. Ebina A. Klip 1998 GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay FEBS Lett. 427 193 197 1:CAS:528:DyaK1cXjtVKjtLc%3D 10.1016/S0014-5793(98)00423-2 9607310 (Pubitemid 28225056)
    • (1998) FEBS Letters , vol.427 , Issue.2 , pp. 193-197
    • Wang, Q.1    Khayat, Z.2    Kishi, K.3    Ebina, Y.4    Klip, A.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.