Microcomputed tomographyg-based structural analysis of various bone tissue regeneration models

Nature Protocols

Volumn 6, Issue 1, 2011, Pages 105-110

  Kallai, Ilan ^a   Mizrahi, Olga ^a   Tawackoli, Wafa ^b   Gazit, Zulma ^a,b   Pelled, Gadi ^a,b   Gazit, Dan ^a,b  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b CEDARS SINAI MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE DEFECT; BONE REGENERATION; BONE REMODELING; BONE STRUCTURE; BONE TISSUE; CONTROLLED STUDY; IMAGE ANALYSIS; LONG BONE; MESENCHYMAL STEM CELL; MICRO-COMPUTED TOMOGRAPHY; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SKULL; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING; ANIMAL; BIOLOGICAL MODEL; BONE; METHODOLOGY; PATHOLOGY; PHYSIOLOGY; ULTRASTRUCTURE;

ANIMALS; BONE AND BONES; BONE REGENERATION; MICE; MODELS, BIOLOGICAL; X-RAY MICROTOMOGRAPHY;

EID: 78651108169     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.180     Document Type: Article

References (16)

1. Kimelman, N., Pelled, G., Gazit, Z. & Gazit, D. Applications of gene therapy and adult stem cells in bone bioengineering. Regen. Med. 1, 549-561 (2006).
2. Holdsworth, D.W. & Thornton, M.M. Micro-CT in small animal and specimen imaging. Trends Biotechnol. 20, S34-S39 (2002).
3. Umoh, J.U. et al. In vivo micro-CT analysis of bone remodeling in a rat calvarial defect model. Phys. Med. Biol. 54, 2147-2161 (2009).
4. Yeung, H. et al. Protocols of micro-computed tomographic analysis established for musculoskeletal applications. In A Practical Manual for Musculoskeletal Research (eds. Leung, K., Qin, L. & Cheung, W.) 279-300 (World Scientific Publishing, Singapore, 2008).
5. Kallai, I. et al. Quantitative, structural, and image-based mechanical analysis of nonunion fracture repaired by genetically engineered mesenchymal stem cells. J. Biomech. 43, 2315-2320 (2010).
6. Kimelman-Bleich, N. et al. The use of a synthetic oxygen carrier-enriched hydrogel to enhance mesenchymal stem cell-based bone formation in vivo. Biomaterials 30, 4639-4648 (2009).
7. Pelled, G. et al. Structural and nanoindentation studies of stem cell-based tissue-engineered bone. J. Biomech. 40, 399-411 (2007).
8. Steinhardt, Y. et al. Maxillofacial-derived stem cells regenerate critical mandibular bone defect. Tissue Eng. Part A 14, 1763-1773 (2008).
9. Zilberman, Y. et al. Fluorescence molecular tomography enables in vivo visualization and quantification of nonunion fracture repair induced by genetically engineered mesenchymal stem cells. J. Orthop. Res. 26, 522-530 (2008).
10. Moutsatsos, I.K. et al. Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol. Ther. 3, 449-461 (2001).
11. Turgeman, G. et al. Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy. J. Gene Med. 3, 240-251 (2001).
12. Tai, K. et al. Nanobiomechanics of repair bone regenerated by genetically modified mesenchymal stem cells. Tissue Eng. Part A 14, 1709-1720 (2008).
13. Gafni, Y. et al. Gene therapy platform for bone regeneration using an exogenously regulated, AAV-2-based gene expression system. Mol. Ther. 9, 587-595 (2004).
14. Hildebrand, T., Laib, A., Muller, R., Dequeker, J. & Ruegsegger, P. Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J. Bone Miner. Res. 14, 1167-1174 (1999).
15. Bouxsein, M.L. et al. Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J. Bone Miner. Res. 25, 1468-1486 (2010).
16. Odgaard, A. & Gundersen, H.J. Quantification of connectivity in cancellous bone, with special emphasis on 3-D reconstructions. Bone 14, 173-182 (1993).