Quantitative, structural, and image-based mechanical analysis of nonunion fracture repaired by genetically engineered mesenchymal stem cells

Journal of Biomechanics

Volumn 43, Issue 12, 2010, Pages 2315-2320

  Kallai, Ilan ^a   van Lenthe, G Harry ^b,c   Ruffoni, Davide ^b   Zilberman, Yoram ^a   Müller, Ralph ^b   Pelled, Gadi ^a,d   Gazit, Dan ^a,d  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b ETH ZURICH   (Switzerland)

^c UNIVERSITY OF LEUVEN   (Belgium)

^d CEDARS SINAI MEDICAL CENTER   (United States)

Author keywords

Bone tissue regeneration; Mesenchymal stem cells; Micro computed tomography; Micro finite element model

Indexed keywords

AXIAL STIFFNESS; BIOMECHANICAL PROPERTIES; BONE FORMATION; BONE GROWTH; BONE TISSUE; BONE TISSUE REGENERATION; BONE VOLUME; FINITE ELEMENT; FINITE ELEMENT MODELS; FRACTURE REPAIR; HIGH RESOLUTION; IMAGE-BASED; IN-BETWEEN; MECHANICAL ANALYSIS; MESENCHYMAL STEM CELL; MICRO ARCHITECTURES; MICRO COMPUTED TOMOGRAPHY (MICRO-CT); MICROCOMPUTED TOMOGRAPHY; MINERAL DENSITY; POST TREATMENT; RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 (RHBMP-2); SKELETAL TISSUES; STEM CELL; TEMPORAL CHANGE;

BIOMECHANICS; BONE; CELL CULTURE; COMPUTERIZED TOMOGRAPHY; FINITE ELEMENT METHOD; FLOWCHARTING; FRACTURE; GENE THERAPY; GENETIC ENGINEERING; NANOINDENTATION; REPAIR;

STEM CELLS;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOMECHANICS; BONE MINERAL; BONE REGENERATION; BONE REMODELING; CALLUS; CONTROLLED STUDY; FEMALE; FINITE ELEMENT ANALYSIS; FRACTURE HEALING; FRACTURE NONUNION; IMAGE ANALYSIS; MESENCHYMAL STEM CELL TRANSPLANTATION; MICRO-COMPUTED TOMOGRAPHY; MOUSE; MUSCULOSKELETAL STIFFNESS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RADIUS; TISSUE STRUCTURE; ULNA;

ANIMALS; BIOMECHANICS; BONE DENSITY; BONE MORPHOGENETIC PROTEIN 2; BONE REGENERATION; BONE REMODELING; DISEASE MODELS, ANIMAL; ELASTIC MODULUS; FEMALE; FINITE ELEMENT ANALYSIS; FRACTURES, UNUNITED; GENE THERAPY; GENETIC ENGINEERING; HUMANS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STEM CELLS; MICE; MICE, INBRED C3H; RADIUS FRACTURES; RECOMBINANT PROTEINS; X-RAY MICROTOMOGRAPHY;

EID: 77955556721     PISSN: 00219290     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiomech.2010.04.031     Document Type: Article

References (21)

1. Arbenz P., van Lenthe G.H., Mennel U., Müller R., Sala M. A scalable multi-level preconditioner for matrix-free γ-finite element analysis of human bone structures. Int. J. Numer. Methods Eng. 2008, 73:927-947.
2. Gazit D., Turgeman G., Kelley P., Wang E., Jalenak M., Zilberman Y., Moutsatsos I. Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. J. Gene Med. 1999, 1:121-133.
3. Hasharoni A., Zilberman Y., Turgeman G., Helm G.A., Liebergall M., Gazit D. Murine spinal fusion induced by engineered mesenchymal stem cells that conditionally express bone morphogenetic protein-2. J. Neurosurg. Spine 2005, 3:47-52.
4. Hildebrand T., Laib A., Müller R., Dequeker J., Ruegsegger P. Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J. Bone Miner. Res. 1999, 14:1167-1174.
5. Homminga J., McCreadie B.R., Weinans H., Huiskes R. The dependence of the elastic properties of osteoporotic cancellous bone on volume fraction and fabric. J. Biomech. 2003, 36:1461-1467.
6. Hsu W.K., Sugiyama O., Park S.H., Conduah A., Feeley B.T., Liu N.Q., Krenek L., Virk M.S., An D.S., Chen I.S., Lieberman J.R. Lentiviral-mediated bmp-2 gene transfer enhances healing of segmental femoral defects in rats. Bone 2007, 40:931-938.
7. Kabel J., van Rietbergen B., Dalstra M., Odgaard A., Huiskes R. The role of an effective isotropic tissue modulus in the elastic properties of cancellous bone. J. Biomech. 1999, 32:673-680.
8. Ladd A.J., Kinney J.H., Haupt D.L., Goldstein S.A. Finite-element modeling of trabecular bone: comparison with mechanical testing and determination of tissue modulus. J. Orthop. Res. 1998, 16:622-628.
9. Lieberman J.R., Le L.Q., Wu L., Finerman G.A., Berk A., Witte O.N., Stevenson S. Regional gene therapy with a bmp-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents. J. Orthop. Res. 1998, 16:330-339.
10. Miller L.M., Little W., Schirmer A., Sheik F., Busa B., Judex S. Accretion of bone quantity and quality in the developing mouse skeleton. J. Bone Miner. Res. 2007, 22:1037-1045.
11. Morgan E.F., Mason Z.D., Chien K.B., Pfeiffer A.J., Barnes G.L., Einhorn T.A., Gerstenfeld L.C. Micro-computed tomography assessment of fracture healing: relationships among callus structure, composition, and mechanical function. Bone 2009, 44:335-344.
12. Moutsatsos I.K., Turgeman G., Zhou S., Kurkalli B.G., Pelled G., Tzur L., Kelley P., Stumm N., Mi S., Müller R., Zilberman Y., Gazit D. Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol. Ther. 2001, 3:449-461.
13. Müller R., Ruegsegger P. Micro-tomographic imaging for the nondestructive evaluation of trabecular bone architecture. Stud. Health Technol. Inf. 1997, 40:61-79.
14. Müller R., Van Campenhout H., Van Damme B., Van Der Perre G., Dequeker J., Hildebrand T., Ruegsegger P. Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone 1998, 23:59-66.
15. Shefelbine S.J., Simon U., Claes L., Gold A., Gabet Y., Bab I., Müller R., Augat P. Prediction of fracture callus mechanical properties using micro-CT images and voxel-based finite element analysis. Bone 2005.
16. Sheyn D., Pelled G., Zilberman Y., Talasazan F., Frank J.M., Gazit D., Gazit Z. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells 2008, 26:1056-1064.
17. Tai K., Pelled G., Sheyn D., Bershteyn A., Han L., Kallai I., Zilberman Y., Ortiz C., Gazit D. Nanobiomechanics of repair bone regenerated by genetically modified mesenchymal stem cells. Tissue Eng. Part A 2008, 14:1709-1720.
18. Turgeman G., Pittman D.D., Muller R., Kurkalli B.G., Zhou S., Pelled G., Peyser A., Zilberman Y., Moutsatsos I.K., Gazit D. Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy. J. Gene Med. 2001, 3:240-251.
19. van Lenthe G.H., Voide R., Boyd S.K., Muller R. Tissue modulus calculated from beam theory is biased by bone size and geometry: implications for the use of three-point bending tests to determine bone tissue modulus. Bone 2008, 43:717-723.
20. van Rietbergen B., Weinans H., Huiskes R., Odgaard A. A new method to determine trabecular bone elastic properties and loading using micromechanical finite-element models. J. Biomech. 1995, 28:69-81.
21. Zilberman Y., Kallai I., Gafni Y., Pelled G., Kossodo S., Yared W., Gazit D. Fluorescence molecular tomography enables in vivo visualization and quantification of nonunion fracture repair induced by genetically engineered mesenchymal stem cells. J. Orthop. Res. 2008, 26:522-530.