Bacteriophage treatment significantly reduces viable Clostridium difficile and prevents toxin production in an in vitro model system

Anaerobe

Volumn 16, Issue 6, 2010, Pages 549-554

  Meader, Emma ^a,b   Mayer, Melinda J ^a   Gasson, Michael J ^a   Steverding, Dietmar ^b   Carding, Simon R ^a   Narbad, Arjan ^a  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

Bacteriophage; Clostridium difficile; Phage

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; METRONIDAZOLE;

ARTICLE; BACTERIAL CELL; BACTERIAL COLONIZATION; BACTERIAL GROWTH; BACTERIAL VIABILITY; BACTERIOPHAGE; BACTEROIDES; BATCH FERMENTATION; BIFIDOBACTERIUM; CLOSTRIDIUM; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; DRUG EFFECT; ENTEROBACTERIACEAE; IN VITRO STUDY; LACTOBACILLUS; NONHUMAN; PRIORITY JOURNAL; TOXIN SYNTHESIS; VIROTHERAPY;

BACTERIAL PROTEINS; BACTERIAL TOXINS; BACTERIOPHAGES; BIOLOGICAL THERAPY; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; MICROBIAL VIABILITY;

BACTEROIDES SP.; BIFIDOBACTERIUM SP.; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM SP.; ENTEROBACTERIACEAE; LACTOBACILLUS SP.;

EID: 78650574888     PISSN: 10759964     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2010.08.006     Document Type: Article

References (36)

1. Collins P., Rhodes J. Ulcerative colitis: diagnosis and management. BMJ 2006, 333(7563):340-343.
2. Health Protection Agency T. Clostridium difficile infections continue to fall [cited 31.07.2009]; Available from:. http://www.hpa.org.uk/webw/HPAweb%26HPAwebPrinterfriendly/HPAweb_C/1239781746891%3Fp%3D1231252394302.
3. National Statistics O f. reported deaths involving Clostridium difficile rise by 28% [cited 30.07.2009]; Available from:. http://www.statistics.gov.uk/pdfdir/mrsacdf0808.pdf.
4. McFarland L.V. Update on the changing epidemiology of Clostridium difficile-associated disease. Nat Clin Pract Gastroenterol Hepatol 2008, 5(1):40-48.
5. Bonten M.J., Willems R., Weinstein R.A. Vancomycin-resistant enterococci: why are they here, and where do they come from?. Lancet Infect Dis 2001, 1(5):314-325.
6. Louie T.J., Meddings J. Clostridium difficile infection in hospitals: risk factors and responses. CMAJ 2004, 171(1):45-46.
7. Pindera L. C. difficile inquest too narrow as " Quebec strain" goes international. CMAJ 2007, 176(7):915-916.
8. Pepin J., Valiquette L., Alary M.E., Villemure P., Pelletier A., Forget K., et al. Clostridium difficile-associated diarrhea in a region of Quebec from 1991 to 2003: a changing pattern of disease severity. CMAJ 2004, 171(5):466-472.
9. Kim K.H., Fekety R., Batts D.H., Brown D., Cudmore M., Silva J., et al. Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis. J Infect Dis 1981, 143(1):42-50.
10. Ahmad S.I. Treatment of post-burns bacterial infections by bacteriophages, specifically ubiquitous Pseudomonas spp. notoriously resistant to antibiotics. Med Hypotheses 2002, 58(4):327-331.
11. Hagens S., Habel A., von Ahsen U., von Gabain A., Blasi U. Therapy of experimental pseudomonas infections with a nonreplicating genetically modified phage. Antimicrob Agents Chemother 2004, 48(10):3817-3822.
12. Mann N.H. The potential of phages to prevent MRSA infections. Res Microbiol 2008, 159(5):400-405.
13. Matsuzaki S., Yasuda M., Nishikawa H., Kuroda M., Ujihara T., Shuin T., et al. Experimental protection of mice against lethal Staphylococcus aureus infection by novel bacteriophage phi MR11. J Infect Dis 2003, 187(4):613-624.
14. Miedzybrodzki R., Fortuna W., Weber-Dabrowska B., Gorski A. Phage therapy of staphylococcal infections (including MRSA) may be less expensive than antibiotic treatment. Postepy Hig Med Dosw (Online) 2007, 61:461-465.
15. Uchiyama J., Rashel M., Takemura I., Wakiguchi H., Matsuzaki S. In silico and in vivo evaluation of bacteriophage phi EF24C, a candidate for treatment of Enterococcus faecalis infections. Appl Environ Microbiol 2008, 74(13):4149-4163.
16. Atterbury R.J., Van Bergen M.A., Ortiz F., Lovell M.A., Harris J.A., De Boer A., et al. Bacteriophage therapy to reduce salmonella colonization of broiler chickens. Appl Environ Microbiol 2007, 73(14):4543-4549.
17. Raya R.R., Varey P., Oot R.A., Dyen M.R., Callaway T.R., Edrington T.S., et al. Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157: H7 levels in sheep. Appl Environ Microbiol 2006, 72(9):6405-6410.
18. Carrillo C.L., Atterbury R.J., El-Shibiny A., Connerton P.L., Dillon E., Scott A., et al. Bacteriophage therapy to reduce Campylobacter jejuni colonization of broiler chickens. Appl Environ Microbiol 2005, 71(11):6554-6563.
19. Sulakvelidze A., Alavidze Z., Morris J.G. Bacteriophage therapy. Antimicrob Agents Chemother 2001, 45(3):649-659.
20. Fortier L.C., Moineau S. Morphological and genetic diversity of temperate phages in Clostridium difficile. Appl Environ Microbiol 2007, 73(22):7358-7366.
21. Goh S., Ong P.F., Song K.P., Riley T.V., Chang B.J. The complete genome sequence of Clostridium difficile phage phiC2 and comparisons to phiCD119 and inducible prophages of CD630. Microbiology 2007, 153(Pt 3):676-685.
22. Goh S., Riley T.V., Chang B.J. Isolation and characterization of temperate bacteriophages of Clostridium difficile. Appl Environ Microbiol 2005, 71(2):1079-1083.
23. Govind R., Fralick J.A., Rolfe R.D. Genomic organization and molecular characterization of Clostridium difficile bacteriophage PhiCD119. J Bacteriol 2006, 188(7):2568-2577.
24. Horgan M, O'Sullivan O, Coffey A, Fitzgerald GF, van Sinderen D, McAuliffe O, et al., Genome analysis of the Clostridium difficile phage PhiCD6356, a temperate phage of the Siphoviridae family. Gene. 462(1-2): p. 34-43.
25. Mahony D.E., Bell P.D., Easterbrook K.B. Two bacteriophages of Clostridium difficile. J Clin Microbiol 1985, 21(2):251-254.
26. Mayer M.J., Narbad A., Gasson M.J. Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin. J Bacteriol 2008, 190(20):6734-6740.
27. Nagy E., Foldes J. Electron microscopic investigation of lysogeny of Clostridium difficile strains isolated from antibiotic-associated diarrhea cases and from healthy carriers. APMIS 1991, 99(4):321-326.
28. Sell T.L., Schaberg D.R., Fekety F.R. Bacteriophage and Bacteriocin typing Scheme for Clostridium-difficile. J Clin Microbiol 1983, 17(6):1148-1152.
29. Ramesh V., Fralick J.A., Rolfe R.D. Prevention of Clostridium difficile-induced ileocecitis with bacteriophage. Anaerobe 1999, 5(2):69-78.
30. Mandalari G., Nueno Palop C., Tuohy K., Gibson G.R., Bennett R.N., Waldron K.W., et al. In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel. Appl Microbiol Biotechnol 2007, 73(5):1173-1179.
31. Freeman J., Baines S.D., Saxton K., Wilcox M.H. Effect of metronidazole on growth and toxin production by epidemic Clostridium difficile PCR ribotypes 001 and 027 in a human gut model. J Antimicrob Chemother 2007, 60(1):83-91.
32. Rea M.C., Clayton E., O'Connor P.M., Shanahan F., Kiely B., Ross R.P., et al. Antimicrobial activity of lacticin 3147 against clinical Clostridium difficile strains. J Med Microbiol 2007, 56(7):940-946.
33. Mutters R., Nonnenmacher C., Susin C., Albrecht U., Kropatsch R., Schumacher S. Quantitative detection of Clostridium difficile in hospital environmental samples by real-time polymerase chain reaction. J Hosp Infect 2009, 71(1):43-48.
34. Serra-Moreno R., Acosta S., Hernalsteens J.P., Jofre J., Muniesa M. Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes. Bmc Mol Biol 2006, 7.
35. Lindemann B.F., Klug C., Schwienhorst A. Evolution of bacteriophage in continuous culture: a model system to test antiviral gene therapies for the emergence of phage escape mutants. J Virol 2002, 76(11):5784-5792.
36. Domingo E., Martinez-Salas E., Sobrino F., de la Torre J.C., Portela A., Ortin J., et al. The quasispecies (extremely heterogeneous) nature of viral RNA genome populations: biological relevance-a review. Gene 1985, 40(1):1-8.