Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin

Journal of Bacteriology

Volumn 190, Issue 20, 2008, Pages 6734-6740

  Mayer, Melinda J ^a   Narbad, Arjan ^a   Gasson, Michael J ^a  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIOPHAGE; CLOSTRIDIUM DIFFICILE; ENDOLYSIN; FIRMICUTES; GENE EXPRESSION; GENE SEQUENCE; INTESTINE FLORA; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

ANTI-BACTERIAL AGENTS; BACTERIOPHAGES; CLONING, MOLECULAR; CLOSTRIDIUM DIFFICILE; DNA, VIRAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE EXPRESSION; GENE ORDER; GENOME, VIRAL; SEQUENCE ANALYSIS, DNA; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM DIFFICILE; ESCHERICHIA COLI; FIRMICUTES; LACTOCOCCUS LACTIS;

EID: 53849135601     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00686-08     Document Type: Article

References (50)

1. Altschul, S. F., T. L. Madden, A. A. Schäffer, Z. Zhang, J. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Babcock, G. J., T. J. Broering, H. J. Hernandez, R. B. Mandell, K. Donahue, N. Boatright, A. M. Stack, I. Lowy, R. Graziano, D. Molrine, D. M. Ambrosino, and W. D. Thomas. 2006. Human monoclonal antibodies directed against toxins A and B prevent Clostridium difficile-induced mortality in hamsters. Infect. Immun. 74:6339-6347.
3. Barker, R. H., R. Dagher, D. M. Davidson, and J. K. Marquis. 2006. Tolevamer, a novel toxin-binding polymer: overview of preclinical pharmacology and physicochemical properties. Aliment. Pharmacol. Ther. 24:1525-1534.
4. Bartlett, J. G. 2006. The new epidemic of Clostridium difficile-associated enteric disease. Ann. Intern. Med. 145:758-764.
5. Braat, H., P. Rottiers, D. W. Hommes, N. Huyghebaert, E. Remaut, J. P. Remon, S. J. H. Van Deventer, S. Neirynck, M. P. Peppelenbosch, and L. Steidler. 2006. A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease. Clin. Gastroenterol. Hepatol. 4:754-759.
6. Carver, T. J., K. M. Rutherford, M. Berriman, M. A. Rajandream, B. G. Barrell, and J. Parkhill. 2005. ACT: the Artemis Comparison Tool. Bioinformatics 21:3422-3423.
7. Casjens, S. R. 2005. Comparative genomics and evolution of the tailed-bacteriophages. Curr. Opin. Microbiol. 8:451-458.
8. Donovan, D. M., S. Dong, W. Garrett, G. M. Rousseau, S. Moineau, and D. G. Pritchard. 2006. Peptidoglycan hydrolase fusions maintain their parental specificities. Appl. Environ. Microbiol. 72:2988-2996.
9. Eckburg, P. B., E. M. Bik, C. N. Bernstein, E. Purdom, L. Dethlefsen, M. Sargent, S. R. Gill, K. E. Nelson, and D. A. Relman. 2005. Diversity of the human intestinal microbial flora. Science 308:1635-1638.
10. Elliott, B., B. J. Chang, C. L. Golledge, and T. V. Riley. 2007. Clostridium difficile-associated diarrhoea. Intern. Med. J. 37:561-568.
11. Fischetti, V. A. 2005. Bacteriophage lytic enzymes: novel anti-infectives. Trends Microbiol. 13:491-496.
12. Fortier, L. C., and S. Moineau. 2007. Morphological and genetic diversity of temperate phages in Clostridium difficile. Appl. Environ. Microbiol. 73:7358-7366.
13. Gaeng, S., S. Scherer, H. Neve, and M. J. Loessner. 2000. Gene cloning and expression and secretion of Listeria monocytogenes bacteriophage-lytic enzymes in Lactococcus lactis. Appl. Environ. Microbiol. 66:2951-2958.
14. Gasson, M. J. October 1992. Bacteriophage lysins and their applications in destroying and testing for bacteria. European patent EP0510907.
15. Gasson, M. J. April 1991. Lysins from bacteriophages. United Kingdom patent GB2255561B.
16. Gasson, M. J. June 1998. Viral products. U.S. patent 5,763,251.
17. Goh, S., P. F. Ong, K. P. Song, T. V. Riley, and B. J. Chang. 2007. The complete genome sequence of Clostridium difficile phage ΦC2 and comparisons to ΦCD119 and inducible prophages of CD630. Microbiology 153:676-685.
18. Goh, S., T. V. Riley, and B. J. Chang. 2005. Isolation and characterization of temperate bacteriophages of Clostridium difficile. Appl. Environ. Microbiol. 71:1079-1083.
19. Govind, R., J. A. Fralick, and R. D. Rolfe. 2006. Genomic organization and molecular characterization of Clostridium difficile bacteriophage ΦCD119. J. Bacteriol. 188:2568-2577.
20. Hanniffy, S. B., A. T. Carter, E. Hitchin, and J. M. Wells. 2007. Mucosal delivery of a pneumococcal vaccine using Lactococcus lactis affords protection against respiratory infection. J. Infect. Dis. 195:185-193.
21. Hermoso, J. A., J. L. Garcia, and P. Garcia. 2007. Taking aim on bacterial pathogens: from phage therapy to enzybiotics. Curr. Opin. Microbiol. 10: 461-472.
22. Horn, N., U. Wegmann, A. Narbad, and M. J. Gasson. 2005. Characterisation of a novel plasmid p9785S from Lactobacillus johnsonii FI9785. Plasmid 54:176-183.
23. Jado, I., R. Lopez, E. Garcia, A. Fenoll, J. Casal, and P. Garcia. 2003. Phage lytic enzymes as therapy for antibiotic-resistant Streptococcus pneumoniae infection in a murine sepsis model. J. Antimicrob. Chemother. 52:967-973.
24. Kretzer, J. W., R. Lehmann, M. Schmelcher, M. Banz, K. P. Kim, C. Korn, and M. J. Loessner. 2007. Use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. Appl. Environ. Microbiol. 73:1992-2000.
25. Loeffler, J. M., D. Nelson, and V. A. Fischetti. 2001. Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 294:2170-2172.
26. Loessner, M. J. 2005. Bacteriophage endolysins - current state of research and applications. Curr. Opin. Microbiol. 8:480-487.
27. Mahony, D. E., P. D. Bell, and K. B. Easterbrook. 1985. Two bacteriophages of Clostridium difficile. J. Clin. Microbiol. 21:251-254.
28. Monaghan, T., T. Boswell, and Y. R. Mahida. 2008. Recent advances in Clostridium difficile-associated disease. Gut 57:850-860.
29. Nagy, E., and J. Foldes. 1991. Electron microscopic investigation of lysogeny of Clostridium difficile strains isolated from antibiotic-associated diarrhea cases and from healthy carriers. APMIS 99:321-326.
30. Nelson, D., L. Loomis, and V. A. Fischetti. 2001. Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme. Proc. Natl. Acad. Sci. USA 98:4107-4112.
31. Nouaille, S., L. A. Ribeiro, A. Miyoshi, D. Pontes, Y. Le Loir, S. C. Oliveira, P. Langella, and V. Azevedo. 2003. Heterologous protein production and delivery systems for Lactococcus lactis. Genet. Mol. Res. 2:102-111.
32. O'Flaherty, S., A. Coffey, W. Meaney, G. F. Fitzgerald, and R. P. Ross. 2005. The recombinant phage lysin LysK has a broad spectrum of lytic activity against clinically relevant staphylococci, including methicillin-resistant Staphylococcus aureus. J. Bacteriol. 187:7161-7164.
33. Parisien, A., B. Allain, J. Zhang, R. Mandeville, and C. Q. Lan. 2008. Novel alternatives to antibiotics: bacteriophages, bacterial cell wall hydrolases, and antimicrobial peptides. J. Appl. Microbiol. 104:1-13.
34. Petty, N. K., T. J. Evans, P. C. Fineran, and G. P. Salmond. 2007. Biotechnological exploitation of bacteriophage research. Trends Biotechnol. 25:7-15.
35. Ramesh, V., J. A. Fralick, and R. D. Rolfe. 1999. Prevention of Clostridium difficile-induced ileocecitis with bacteriophage. Anaerobe 5:69-78.
36. Rashel, M., J. Uchiyama, T. Ujihara, Y. Uehara, S. Kuramoto, S. Sugihara, K. Yagyu, A. Muraoka, M. Sugai, K. Hiramatsu, K. Honke, and S. Matsuzaki. 2007. Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage ΦMR11. J. Infect. Dis. 196:1237-1247.
37. Rutherford, K., J. Parkhill, J. Crook, T. Horsnell, P. Rice, M.-A. Rajandream, and B. Barrell. 2000. Artemis: sequence visualisation and annotation. Bioinformatics 16:944-945.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
39. Sass, P., and G. Bierbaum. 2007. Lytic activity of recombinant bacteriophage Φ11 and Φ12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl. Environ. Microbiol. 73:347-352.
40. Schuch, R., D. Nelson, and V. A. Fischetti. 2002. A bacteriolytic agent that detects and kills Bacillus anthracis. Nature 418:884-889.
41. Sebaihia, M., M. W. Peck, N. P. Minton, N. R. Thomson, M. T. Holden, W. J. Mitchell, A. T. Carter, S. D. Bentley, D. R. Mason, L. Crossman, C. J. Paul, A. Ivens, M. H. Wells-Bennik, I. J. Davis, A. M. Cerdeno-Tarraga, C. Churcher, M. A. Quail, T. Chillingworth, T. Feltwell, A. Fraser, I. Goodhead, Z. Hance, K. Jagels, N. Larke, M. Maddison, S. Moule, K. Mungall, H. Norbertczak, E. Rabbinowitsch, M. Sanders, M. Simmonds, B. White, S. Whithead, and J. Parkhill. 2007. Genome sequence of a proteolytic (group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes. Genome Res. 17:1082-1092.
42. Sebaihia, M., B. W. Wren, P. Mullany, N. F. Fairweather, N. Minton, R. Stabler, N. R. Thomson, A. P. Roberts, A. M. Cerdeno-Tarraga, H. Wang, M. T. Holden, A. Wright, C. Churcher, M. A. Quail, S. Baker, N. Bason, K. Brooks, T. Chillingworth, A. Cronin, P. Davis, L. Dowd, A. Fraser, T. Feltwell, Z. Hance, S. Holroyd, K. Jagels, S. Moule, K. Mungall, C. Price, E. Rabbinowitsch, S. Sharp, M. Simmonds, K. Stevens, L. Unwin, S. Whithead, B. Dupuy, G. Dougan, B. Barrell, and J. Parkhill. 2006. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38:779-786.
43. Sell, T. L., D. R. Schaberg, and F. R. Fekety. 1983. Bacteriophage and bacteriocin typing scheme for Clostridium difficile. J. Clin. Microbiol. 17: 1148-1152.
44. Steidler, L., W. Hans, L. Schotte, S. Neirynck, F. Obermeier, W. Falk, W. Fiers, and E. Remaut. 2000. Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science 289:1352-1355.
45. Voth, D. E., and J. D. Ballard. 2005. Clostridium difficile toxins: mechanism of action and role in disease. Clin. Microbiol. Rev. 18:247-263.
46. Wegmann, U., J. R. Klein, I. Drumm, O. P. Kuipers, and B. Henrich. 1999. Introduction of peptidase genes from Lactobacillus delbrueckii subsp. lactis into Lactococcus lactis and controlled expression. Appl. Environ. Microbiol. 65:4729-4733.
47. Yoong, P., R. Schuch, D. Nelson, and V. A. Fischetti. 2004. Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium. J. Bacteriol. 186:4808-4812.
48. Yuvaraj, S., M. P. Peppelenbosch, and N. A. Bos. 2007. Transgenic probiotica as drug delivery systems: the golden bullet? Expert Opin. Drug Deliv. 4:1-3.
49. Zdobnov, E. M., and R. Apweiler. 2001. InterProScan: an integration platform for the signature-recognition methods in InterPro. Bioinformatics 17: 847-848.
50. Zimmer, M., N. Vukov, S. Scherer, and M. J. Loessner. 2002. The murein hydrolase of the bacteriophage Φ3626 dual lysis system is active against all tested Clostridium perfringens strains. Appl. Environ. Microbiol. 68:5311-5317.