Using NMR spectroscopy to obtain the higher order structure of biopharmaceutical products

BioPharm International

Volumn 23, Issue 8 SUPPL., 2010, Pages 28-34

  Aubin, Yves ^a   Jones, Christopher ^b   Freedberg, Darón I ^c  

^a HEALTH CANADA   (Canada)

^b NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

^c CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIPHTHERIA TOXOID CRM197; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; POLYSACCHARIDE VACCINE;

ACCURACY; ARTICLE; COST EFFECTIVENESS ANALYSIS; DRUG DETERMINATION; DRUG INDUSTRY; DRUG MANUFACTURE; HETERONUCLEAR SINGLE QUANTUM COHERENCE; INTERMETHOD COMPARISON; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH MEASUREMENT; PROTEIN STRUCTURE; QUALITY CONTROL; SENSITIVITY ANALYSIS; STRUCTURE ANALYSIS; VACCINE PRODUCTION;

EID: 78650493200     PISSN: 1542166X     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Article

References (10)

1. Abeygunawardana C, Williams TC, Sumner JS, Hennessey JP. Development and validation of an NMR-based identity assay for bacterial polysaccharides. Anal Biochem. 2000;279:226-40.
2. Cavanagh J, Fairbrother W, Palmer A, Rance M, Skelton N. Protein NMR Spectroscopy, 2nd Ed. Academic Press: San Diego, 2007.
3. Frasch CE, Concepcion N. Induction of group 17 specifi antibodies by pneumococcal type 17F and 17A polysaccharide vaccines. Biologicals. 2001;29(1):11-16.
4. C. Jones. Vaccines based on the cellsurface carbohydrates of pathogenic bacteria. An Bras Acad Cienc. 2005;77:293-324.
5. Mawas F, Niggemann J, Jones C, Corbel MJ, Kamerling JP, Vliegenthart JFP. A conjugate vaccine made with a synthetic single repeating unit of Type 14 pneumococcal polysaccharide coupled to CRM197 is immunogenic in a mouse model. Infect Immun. 2002;70:5107-114.
6. Kellenbach E, Sanders K, Overbeeke PLA. The use of proton NMR as an alternative for the amino acid analysis as identity test for peptides. In: Holzgrabe U, Wawer I, Diehls B, editors. NMR spectroscopy in Pharmaceutical analysis. Oxford, UK: Elsevier; 2008. p 429-36
7. Aubin Y, Gingras G, Sauvé S. Assessment of the three-dimensional structure of recombinant protein therapeutics by NMR fingerprinting: demonstration on rhGMCSF. Anal Chem. 2008;80:2623-7.
8. Klaus W, Gsell B, Labhardt AM, Wipf B, Senn H. The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution. J Mol Biol. 1997;274:661-75.
9. Sauvé S, Gingras G, Aubin Y. NMR assignment of backbone and side chain resonannces for human granulocytemacrophage colony-stimulating factor. Biomol NMR Assign. 2008;2:5-7.
10. Panjwani N, Hodgson DJ, Sauvé S, Gingras G, Aubin Y. Assessment of the Effects of pH, Formulation, and Deformulation on the Conformation of Interferon alpha-2 by NMR. J Pharm Sci, Published Online: 23 Feb 2010, DOI 10.1002/jps.22105.