Potential of ultraviolet wide-field imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes

Microscopy Research and Technique

Volumn 74, Issue 1, 2011, Pages 92-108

  Wüstner, Daniel ^a   Brewer, Jonathan R ^a   Bagatolli, Luis ^a   Sage, Daniel ^b  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b EPFL   (Switzerland)

Author keywords

Cholesterol; Fluorescence; Image correlation; Intracellular transport; Microdomain

Indexed keywords

ALCOHOLS; BLEACHING; CELL MEMBRANES; CHOLESTEROL; CLEANING; IMAGE ANALYSIS; IMAGE ENHANCEMENT; LIPIDS; OXIDATION; PHOTOBLEACHING; PROTEINS; SIGNAL TO NOISE RATIO; YARN;

CELLULAR MEMBRANES; DEHYDROERGOSTEROL; IMAGE CORRELATIONS; IMAGING MICROSCOPY; INTRACELLULAR TRANSPORT; MICRO-DOMAINS; MULTIPHOTON MICROSCOPY; MULTIPHOTONS; ULTRAVIOLET REGION; WIDE FIELD IMAGING;

FLUORESCENCE;

EID: 78650313673     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20878     Document Type: Article

References (51)

1. Aït-Slimane T, Galmes R, Trugnan G, Maurice M. 2009. Basolateral internalization of GPI-anchored proteins occurs via a clathrin-independent flotillin-dependent pathway in polarized hepatic cells. Mol Biol Cell 20: 3792-3800.
2. Benson DM, Bryan J, Plant AL, Gotto AMJ, Smith LC. 1985. Digital imaging fluorescence microscopy: Spatial heterogeneity of photobleaching rate constants in individual cells. J Cell Biol 100: 1309-1323.
3. Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW. 2006. Probing the integrin-actin linkage using high-resolution protein velocity mapping. J Cell Sci 119: 5204-5214.
4. Diaz G, Batetta B, Sanna F, Uda S, Reali C, Angius F, Melis M, Falchi AM. 2008. Lipid droplet changes in proliferating and quiescent 3T3 fibroblasts. Histochem Cell Biol 129: 611-621.
5. Digman MA, Dalal R, Horwitz AF, Gratton E. 2008. Mapping the number of molecules and brightness in the laser scanning microscope. Biophys J 94: 2320-2332.
6. Frolov A, Petrescu A, Atshaves BP, So PT, Gratton E, Serrero G, Schroeder F. 2000. High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. J Biol Chem 275: 12769-12780.
7. Garvik O, Benediktsen P, Ipsen JH, Simonsen AC, Wüstner D. 2008. The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes. Chem Phys Lipids 159: 114-118.
8. Gonzalez RC, Woods RE. 2002. Digital image processing. Upper Saddle River, NJ: Prentice Hall. Chapter 11, pp. 675-683.
9. Hao M, Lin SX, Karylowski OJ, Wüstner D, McGraw TE, Maxfield FR. 2002. Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle. J Biol Chem 277: 609-617.
10. Hartwig Petersen N, Færgeman NJ, Yu L, Wüstner D. 2008. Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells. J Lipid Res 49: 2023-2037.
11. Hebert B, Costantino S, Wiseman PW. 2005a. Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells. Biophys J 88: 3601-3614.
12. Hebert B, Hulme SE, Wiseman PW. 2005b. Membrane protein dynamics measured by two-photon ring correlation spectroscopy: Theory and application to living cells. In: Periasamy A, So PT, editors.Multiphoton microscopy in the biomedical sciences V. Proceedings of the SPIE, Vol. 5700. Bellingham, WA: SPIE. pp. 109-117.
13. Hirschfeld T. 1976. Quantum efficiency independence of the time integrated emission from a fluorescent molecule. Appl Opt 15: 3135-3139.
14. Hölttä-Vuori M, Alpy F, Tanhuanpaa K, Jokitalo E, Mutka AL, Ikonen E. 2005. MLN64 is involved in actin-mediated dynamics of late endocytic organelles. Mol Biol Cell 16: 3873-3886.
15. Kolin DL, Wiseman PW. 2007. Advances in image correlation spectroscopy: Measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells. Cell Biochem Biophys 49: 141-164.
16. Kolin DL, Costantino S, Wiseman PW. 2006. Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy. Biophys J 90: 628-639.
17. Luisier F, Vonesch C, Blu T, Unser M. 2010. Fast interscale wavelet denoising of Poisson-corrupted images. Signal Processing 90: 415-427.
18. Markham J, Conchello JA. 2001. Artefacts in restored images due to intensity loss in three-dimensional fluorescence microscopy. J Microsc 204: 93-98.
19. McIntosh AL, Gallegos AM, Atshaves BP, Storey SM, Kannoju D, Schroeder F. 2003. Fluorescence and multiphoton imaging resolve unique structural forms of sterol in membranes of living cells. J Biol Chem 278: 6384-6403.
20. Mondal M, Mesmin B, Mukherjee S, Maxfield FR. 2009. Sterols are mainly in the cytoplasmic leaflet of the plasma membrane and the endocytic recycling compartment in CHO cells. Mol Biol Cell 20: 581-588.
21. Mukherjee S, Zha X, Tabas I, Maxfield FR. 1998. Cholesterol distribution in living cells: Fluorescence imaging using dehydroergosterol as a fluorescent cholesterol analog. Biophys J 75: 1915-1925.
22. Murray JM. 1998. Evaluating the performance of fluorescence microscopes. J Microsc 19: 128-134.
23. Önfelt B, Nedvetzki S, Yanagi K, Davis DM. 2004. Cutting edge: Membrane nanotubes connect immune cells. J Immunol 173: 1511-1513.
24. Petersen NO, Höddelius PL, Wiseman PW, Seger O, Magnusson KE. 1993. Quantitation of membrane receptor distributions by image correlation spectroscopy: Concept and application. Biophys J 65: 1135-1146.
25. Pipalia NH, Hao M, Mukherjee S, Maxfield FR. 2006. Sterol, protein and lipid trafficking in Chinese hamster ovary cells with Niemann-Pick type C1 defect. Traffic 8: 130-141.
26. Rustom A. 2009. Hen or egg?: Some thoughts on tunneling nanotubes. Ann N Y Acad Sci 1178: 129-136.
27. Rustom A, Saffrich R, Markovic I, Walther P, Gerdes H-H. 2004. Nanotubular highways for intercellular organelle transport. Science 303: 1007-1010.
28. Salzmann NH, Maxfield FR. 1989. Fusion accessibility of endocytic compartments along the recycling and lysosomal endocytic pathways in intact cells. J Cell Biol 109: 2097-2104.
29. Scheidt HA, Müller P, Herrmann A, Huster D. 2003. The potential of fluorescent and spin-labeled steroid analogs to mimic natural cholesterol. J Biol Chem 278: 45563-45569.
30. Schrader M, Bahlmann K, Hell SW. 1997. Three-photon-excitation microscopy: Theory, experiment and applications. Optik 104: 116-124.
31. Slimane TA, Trugnan G, van IJzendoorn SC, Hoekstra D. 2003. Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: Role of distinct lipid microdomains. Mol Biol Cell 14: 611-624.
32. Sormunen R, Eskelinen S, Lehto VP. 1993. Bile canaliculus formation in cultured HEPG2 cells. Lab Invest 68: 652-662.
33. Stelzer EHK. 1998. Contrast, resolution, pixelation, dynamic range and signal-to-noise ratio: Fundamental limits to resolution in fluorescence light microscopy. J Microsc 189: 15-24.
34. Tokumasu F, Fairhurst RM, Ostera GR, Brittain NJ, Hwang J, Wellems TE, Dvorak JA. 2005. Band 3 modifications in Plasmodium falciparum-infected AA and CC erythrocytes assayed by autocorrelation analysis using quantum dots. J Cell Sci 118: 1091-1098.
35. van IJzendoorn SC, Hoekstra D. 1998. (Glyco)sphingolipids are sorted in sub-apical compartments in HepG2 cells: A role for non-Golgi related intracellular sites in the polarized distribution of (Glyco)sphingolipids. J Cell Biol 142: 683-696.
36. van IJzendoorn SC, Hoekstra D. 1999. Polarized sphingolipid transport from the subapical compartment: Evidence for distinct sphingolipid domains. Mol Biol Cell 10: 3449-3461.
37. Wiseman PW, Squier JA, Ellisman MH, Wilson KR. 2000. Two-photon image correlation spectroscopy and image cross-correlation spectroscopy. J Microsc 200: 14-25.
38. Wojtal KA, de Vries E, Hoekstra D, van IJzendoorn SC. 2006. Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide. Mol Biol Cell 17: 3638-3650.
39. Wüstner D. 2005. Improved visualization and quantitative analysis of fluorescent membrane sterol in polarized hepatic cells. J Microsc 220: 47-64.
40. Wüstner D. 2007a. Fluorescent sterols as tools in membrane biophysics and cell biology. Chem Phys Lipids 146: 1-25.
41. Wüstner D. 2007b. Plasma membrane sterol distribution resembles the surface topography of living cells. Mol Biol Cell 18: 211-228.
42. Wüstner D. 2008. Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages. Chem Phys Lipids 154: 129-136.
43. Wüstner D, Færgeman NJ. 2008a. Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets. Cytometry A 73: 727-744.
44. Wüstner D, Færgeman NJ. 2008b. Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells. Histochem Cell Biol 130: 891-908.
45. Wüstner D, Mukherjee S, Maxfield FR, Müller P, Hermann A. 2001. Vesicular and nonvesicular transport of phosphatidylcholine in polarized HepG2 cells. Traffic 2: 277-296.
46. Wüstner D, Herrmann A, Hao M, Maxfield FR. 2002. Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells. J Biol Chem 277: 30325-30336.
47. Wüstner D, Mondal M, Huang A, Maxfield FR. 2004. Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells. J Lipid Res 45: 427-437.
48. Wüstner D, Mondal M, Tabas I, Maxfield FR. 2005. Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells. Traffic 6: 396-412.
49. Wüstner D, Landt Larsen A, Færgeman NJ, Brewer JR, Sage D. 2010. Selective visualization of fluorescent sterols in Caenorhabditis elegans by bleach-rate based image segmentation. Traffic 11: 440-454.
50. Zhang W, McIntosh AL, Xu H, Wu D, Gruninger T, Atshaves B, Liu JC, Schroeder F. 2005. Structural analysis of sterol distributions in the plasma membrane of living cells. Biochemistry 44: 2864-2884.
51. Zipfel WR, Williams RM, Webb WW. 2003. Nonlinear magic: Multiphoton microscopy in the biosciences. Nat Biotechnol 21: 1369-1377.