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Volumn 11, Issue 4, 2010, Pages 440-454

Selective visualization of fluorescent sterols in caenorhabditis elegans by bleach-rate-based image segmentation

Author keywords

Bleaching; Cholesterol; Dehydroergosterol; Fluorescence; In vivo imaging; Multiphoton microscopy; Segmentation; Transport

Indexed keywords

ERGOSTEROL; NATURAL CYTOTOXICITY TRIGGERING RECEPTOR 1; STEROL;

EID: 77951780747     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2010.01040.x     Document Type: Article
Times cited : (36)

References (59)
  • 1
    • 14944381287 scopus 로고    scopus 로고
    • HDL as a target in the treatment of artherosclerotic cardiovascular disease.
    • Linsel-Nitschke P, Tall AR. HDL as a target in the treatment of artherosclerotic cardiovascular disease. Nat Rev Drug Disc 2005, 4:193-205.
    • (2005) Nat Rev Drug Disc , vol.4 , pp. 193-205
    • Linsel-Nitschke, P.1    Tall, A.R.2
  • 2
    • 28444479505 scopus 로고    scopus 로고
    • Role of cholesterol and lipid organization in disease
    • Maxfield FR, Tabas I. Role of cholesterol and lipid organization in disease. Nature 2005, 438:612-621.
    • (2005) Nature , vol.438 , pp. 612-621
    • Maxfield, F.R.1    Tabas, I.2
  • 4
    • 37348999201 scopus 로고    scopus 로고
    • Lessons from " lower" organisms: what worms, flies, and zebrafish can teach us about human energy metabolism. PLoS Genet., e199.
    • Schlegel A, Stainier DY. 2007, 3. Lessons from " lower" organisms: what worms, flies, and zebrafish can teach us about human energy metabolism. PLoS Genet., e199.
    • (2007) , vol.3
    • Schlegel, A.1    Stainier, D.Y.2
  • 7
    • 10344229417 scopus 로고    scopus 로고
    • NCR-1 and NCR-2, the C. elegans homologs of the human Niemann-Pick type C1 disease protein, function upstream of DAF-9 in the dauer formation pathways.
    • Li J, Brown G, Ailion M, Lee S, Thomas JH. NCR-1 and NCR-2, the C. elegans homologs of the human Niemann-Pick type C1 disease protein, function upstream of DAF-9 in the dauer formation pathways. Development 2004, 131:5741-5752.
    • (2004) Development , vol.131 , pp. 5741-5752
    • Li, J.1    Brown, G.2    Ailion, M.3    Lee, S.4    Thomas, J.H.5
  • 8
    • 34547844237 scopus 로고    scopus 로고
    • Human NPC1L1 and NPC1 can functionally substitute for the ncr genes to promote reproductive development in C. elegans.
    • Smith MM, Levitan DJ. Human NPC1L1 and NPC1 can functionally substitute for the ncr genes to promote reproductive development in C. elegans. Biochim Biophys Acta 2007, 1770:1345-1351.
    • (2007) Biochim Biophys Acta , vol.1770 , pp. 1345-1351
    • Smith, M.M.1    Levitan, D.J.2
  • 9
    • 0034192462 scopus 로고    scopus 로고
    • A model for niemann-pick type C disease in the nematode Caenorhabditis elegans
    • Sym M, Basson M, Johnson C. A model for niemann-pick type C disease in the nematode Caenorhabditis elegans. Curr Biol 2000, 10:527-530.
    • (2000) Curr Biol , vol.10 , pp. 527-530
    • Sym, M.1    Basson, M.2    Johnson, C.3
  • 10
    • 15944426129 scopus 로고    scopus 로고
    • Requirement of sterols in the life cycle of the nematode Caenorhabditis elegans
    • Entchev EV, Kurzchalia TV. Requirement of sterols in the life cycle of the nematode Caenorhabditis elegans. Semin Cell Dev Biol 2005, 16:175-182.
    • (2005) Semin Cell Dev Biol , vol.16 , pp. 175-182
    • Entchev, E.V.1    Kurzchalia, T.V.2
  • 11
    • 0037244289 scopus 로고    scopus 로고
    • Sterol effects and sites of sterol accumulation in Caenorhabditis elegans: developmental requirement for 4alpha-methyl sterols
    • Merris M, Wadsworth WG, Khamrai U, Bittman R, Chitwood DJ, Lenard J. Sterol effects and sites of sterol accumulation in Caenorhabditis elegans: developmental requirement for 4alpha-methyl sterols. J Lipid Res 2003, 44:172-181.
    • (2003) J Lipid Res , vol.44 , pp. 172-181
    • Merris, M.1    Wadsworth, W.G.2    Khamrai, U.3    Bittman, R.4    Chitwood, D.J.5    Lenard, J.6
  • 13
    • 33846867956 scopus 로고    scopus 로고
    • Fluorescent sterols as tools in membrane biophysics and cell biology
    • Wüstner D. Fluorescent sterols as tools in membrane biophysics and cell biology. Chem Phys Lipids 2007, 146:1-25.
    • (2007) Chem Phys Lipids , vol.146 , pp. 1-25
    • Wüstner, D.1
  • 14
    • 67349204082 scopus 로고    scopus 로고
    • The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes
    • Garvik O, Benediktsen P, Ipsen JH, Simonsen AC, Wüstner D. The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes. Chem Phys Lipids 2008, 159:114-118.
    • (2008) Chem Phys Lipids , vol.159 , pp. 114-118
    • Garvik, O.1    Benediktsen, P.2    Ipsen, J.H.3    Simonsen, A.C.4    Wüstner, D.5
  • 16
    • 61949446361 scopus 로고    scopus 로고
    • Sterols are mainly in the cytoplasmic leaflet of the plasma membrane and the endocytic recycling compartment in CHO cells
    • Mondal M, Mesmin B, Mukherjee S, Maxfield FR. Sterols are mainly in the cytoplasmic leaflet of the plasma membrane and the endocytic recycling compartment in CHO cells. Mol Biol Cell 2009, 20:581-588.
    • (2009) Mol Biol Cell , vol.20 , pp. 581-588
    • Mondal, M.1    Mesmin, B.2    Mukherjee, S.3    Maxfield, F.R.4
  • 17
    • 0037016677 scopus 로고    scopus 로고
    • Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle.
    • Hao M, Lin SX, Karylowski OJ, Wüstner D, McGraw TE, Maxfield FR. Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle. J Biol Chem 2002, 277:609-617.
    • (2002) J Biol Chem , vol.277 , pp. 609-617
    • Hao, M.1    Lin, S.X.2    Karylowski, O.J.3    Wüstner, D.4    McGraw, T.E.5    Maxfield, F.R.6
  • 18
    • 0043198069 scopus 로고    scopus 로고
    • Why do worms need cholesterol?
    • Kurzchalia TV Ward S. Why do worms need cholesterol? Nat Cell Biol 2003, 5:684-688.
    • (2003) Nat Cell Biol , vol.5 , pp. 684-688
    • Kurzchalia, T.V.W.S.1
  • 19
    • 0032863254 scopus 로고    scopus 로고
    • Biochemistry and function of nematode steroids
    • Chitwood DJ. Biochemistry and function of nematode steroids. Crit Rev Biochem Mol Biol 1999, 34:273-284.
    • (1999) Crit Rev Biochem Mol Biol , vol.34 , pp. 273-284
    • Chitwood, D.J.1
  • 20
    • 23844436700 scopus 로고    scopus 로고
    • In vivo spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans
    • Gerstbrein B, Stamatas G, Kollias N, Driscoll M. In vivo spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans. Aging Cell 2005, 4:127-137.
    • (2005) Aging Cell , vol.4 , pp. 127-137
    • Gerstbrein, B.1    Stamatas, G.2    Kollias, N.3    Driscoll, M.4
  • 21
    • 27944453100 scopus 로고    scopus 로고
    • Improved visualization and quantitative analysis of fluorescent membrane sterol in polarized hepatic cells
    • Wüstner D. Improved visualization and quantitative analysis of fluorescent membrane sterol in polarized hepatic cells. J Microsc 2005, 220:47-64.
    • (2005) J Microsc , vol.220 , pp. 47-64
    • Wüstner, D.1
  • 22
    • 48849099794 scopus 로고    scopus 로고
    • Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets.
    • Wüstner D, Færgeman NJ. Cytometry A 2008, 73:727-744. Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets.
    • (2008) Cytometry A , vol.73 , pp. 727-744
    • Wüstner, D.1    Færgeman, N.J.2
  • 25
    • 0017241350 scopus 로고
    • Quantum efficiency independence of the time integrated emission from a fluorescent molecule
    • Hirschfeld T. Quantum efficiency independence of the time integrated emission from a fluorescent molecule. Applied Optics 1976, 15:3135-3139.
    • (1976) Applied Optics , vol.15 , pp. 3135-3139
    • Hirschfeld, T.1
  • 26
    • 0021956248 scopus 로고
    • Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells
    • Benson DM, Bryan J, Plant AL, Gotto AMJ, Smith LC. Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells. J Cell Biol 1985, 100:1309-1323.
    • (1985) J Cell Biol , vol.100 , pp. 1309-1323
    • Benson, D.M.1    Bryan, J.2    Plant, A.L.3    Gotto, A.M.J.4    Smith, L.C.5
  • 27
    • 0023277161 scopus 로고
    • A fluorescence study of dehydroergosterol in phosphatidylcholine bilayer vesicles
    • Schroeder F, Barenholz Y, Gratton E, Thompson TE. A fluorescence study of dehydroergosterol in phosphatidylcholine bilayer vesicles. Biochemistry 1987, 26:2441-2448.
    • (1987) Biochemistry , vol.26 , pp. 2441-2448
    • Schroeder, F.1    Barenholz, Y.2    Gratton, E.3    Thompson, T.E.4
  • 28
    • 0034724682 scopus 로고    scopus 로고
    • High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts
    • Frolov A, Petrescu A, Atshaves BP, So PT, Gratton E, Serrero G, Schroeder F. High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. J Biol Chem 2000, 275:12769-12780.
    • (2000) J Biol Chem , vol.275 , pp. 12769-12780
    • Frolov, A.1    Petrescu, A.2    Atshaves, B.P.3    So, P.T.4    Gratton, E.5    Serrero, G.6    Schroeder, F.7
  • 29
    • 0037458554 scopus 로고    scopus 로고
    • Fluorescence and multiphoton imaging resolve unique structural forms of sterol in membranes of living cells
    • McIntosh AL, Gallegos AM, Atshaves BP, Storey SM, Kannoju D, Schroeder F. Fluorescence and multiphoton imaging resolve unique structural forms of sterol in membranes of living cells. J Biol Chem 2003, 278:6384-6403.
    • (2003) J Biol Chem , vol.278 , pp. 6384-6403
    • McIntosh, A.L.1    Gallegos, A.M.2    Atshaves, B.P.3    Storey, S.M.4    Kannoju, D.5    Schroeder, F.6
  • 31
    • 0242353872 scopus 로고    scopus 로고
    • Nonlinear magic: multiphoton microscopy in the biosciences
    • Zipfel WR, Williams RM, Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 2003, 21:1369-1377.
    • (2003) Nat Biotechnol , vol.21 , pp. 1369-1377
    • Zipfel, W.R.1    Williams, R.M.2    Webb, W.W.3
  • 32
    • 37349058677 scopus 로고    scopus 로고
    • In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy
    • Gualda EJ, Filippidis G, Voglis G, Mari M, Fotakis C, Tavernarakis N. In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy. J Microsc 2008, 229:141-150.
    • (2008) J Microsc , vol.229 , pp. 141-150
    • Gualda, E.J.1    Filippidis, G.2    Voglis, G.3    Mari, M.4    Fotakis, C.5    Tavernarakis, N.6
  • 33
    • 77951808574 scopus 로고    scopus 로고
    • Intracellular cholesterol transport
    • Ehnholm C. In:, editor., Springer press
    • Wüstner D. Intracellular cholesterol transport. Cellular Lipid Metabolism 2009, 157-190. Ehnholm C. In:, editor., Springer press, pp.
    • (2009) Cellular Lipid Metabolism , pp. 157-190
    • Wüstner, D.1
  • 34
    • 0030897905 scopus 로고    scopus 로고
    • Sturcture-oriented fluorescence photobleaching analysis: a method for double fluorescent labeling studies
    • Ghauharali RI, van Driel R, Brakenhoff GJ. Sturcture-oriented fluorescence photobleaching analysis: a method for double fluorescent labeling studies. J Microsc 1997, 185:375-384.
    • (1997) J Microsc , vol.185 , pp. 375-384
    • Ghauharali, R.I.1    van Driel, R.2    Brakenhoff, G.J.3
  • 35
    • 84986720854 scopus 로고
    • Analysis of heterogeneous fluorescence photobleaching by video kinetics imaging: the method of cumulants
    • Koppel DE, Carlson C, Smilowitz H. Analysis of heterogeneous fluorescence photobleaching by video kinetics imaging: the method of cumulants. J Microsc 1989, 155:199-206.
    • (1989) J Microsc , vol.155 , pp. 199-206
    • Koppel, D.E.1    Carlson, C.2    Smilowitz, H.3
  • 36
    • 33645911464 scopus 로고    scopus 로고
    • Sequential photobleaching of fluorochromes for polychromatic slide-based cytometry
    • Mittag A, Lenz D, Bocsi J, Sack U, Gerstner AO, Tárnok A. Sequential photobleaching of fluorochromes for polychromatic slide-based cytometry. Cytometry A 2006, 69:139-141.
    • (2006) Cytometry A , vol.69 , pp. 139-141
    • Mittag, A.1    Lenz, D.2    Bocsi, J.3    Sack, U.4    Gerstner, A.O.5    Tárnok, A.6
  • 37
    • 0022999003 scopus 로고
    • Physical properties of the fluorescent sterol probe dehydroergosterol
    • Smutzer G, Crawford BF, Yeagle PL. Physical properties of the fluorescent sterol probe dehydroergosterol. Biochim et Biophys Acta 1986, 862:361-371.
    • (1986) Biochim et Biophys Acta , vol.862 , pp. 361-371
    • Smutzer, G.1    Crawford, B.F.2    Yeagle, P.L.3
  • 38
    • 0023706928 scopus 로고
    • Time-resolved fluorescence investigation of membrane cholesterol heterogeneity and exchange
    • Nemecz G, FS. Time-resolved fluorescence investigation of membrane cholesterol heterogeneity and exchange. Biochemistry 1988, 27:7740-7749.
    • (1988) Biochemistry , vol.27 , pp. 7740-7749
    • Nemecz, G.1
  • 39
    • 0022999176 scopus 로고
    • Depolarization of dehydroergosterol in phospholipid bilayers
    • Chong PL, Thompson TE. Depolarization of dehydroergosterol in phospholipid bilayers. Biochim Biophys Acta 1986, 863:53-62.
    • (1986) Biochim Biophys Acta , vol.863 , pp. 53-62
    • Chong, P.L.1    Thompson, T.E.2
  • 40
    • 0024400234 scopus 로고
    • Spontaneous transfer between phospholipid bilayers of dehydroergosterol, a fluorescent cholesterol analog
    • Bar LK, Chong PL, Barenholz Y, Thompson TE. Spontaneous transfer between phospholipid bilayers of dehydroergosterol, a fluorescent cholesterol analog. Biochim Biophys Acta 1989, 983:109-112.
    • (1989) Biochim Biophys Acta , vol.983 , pp. 109-112
    • Bar, L.K.1    Chong, P.L.2    Barenholz, Y.3    Thompson, T.E.4
  • 41
    • 0022978434 scopus 로고
    • Fraction of cholesterol undergoing spontaneous exchange between small unilamellar phosphatidylcholine vesicles
    • Bar LK, Barenholz Y, Thompson TE. Fraction of cholesterol undergoing spontaneous exchange between small unilamellar phosphatidylcholine vesicles. Biochemistry 1986, 25:6701-6705.
    • (1986) Biochemistry , vol.25 , pp. 6701-6705
    • Bar, L.K.1    Barenholz, Y.2    Thompson, T.E.3
  • 42
    • 54349098469 scopus 로고    scopus 로고
    • Regulation of sterol transport between membranes and NPC2.
    • Xu Z, Farver W, Kodukula S, Storch J. Regulation of sterol transport between membranes and NPC2. Biochemistry 2008, 47:11134-11143.
    • (2008) Biochemistry , vol.47 , pp. 11134-11143
    • Xu, Z.1    Farver, W.2    Kodukula, S.3    Storch, J.4
  • 43
    • 0242385346 scopus 로고    scopus 로고
    • Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol
    • Veatch SL, Keller SL. Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol. Biophys J 2003, 85:3074-3083.
    • (2003) Biophys J , vol.85 , pp. 3074-3083
    • Veatch, S.L.1    Keller, S.L.2
  • 44
    • 2142651634 scopus 로고    scopus 로고
    • Liquid domains in vesicles investigated by NMR and fluorescence microscopy
    • Veatch SL, Polozov IV, Gawrisch K, Keller SL. Liquid domains in vesicles investigated by NMR and fluorescence microscopy. Biophys J 2004, 86:2910-2922.
    • (2004) Biophys J , vol.86 , pp. 2910-2922
    • Veatch, S.L.1    Polozov, I.V.2    Gawrisch, K.3    Keller, S.L.4
  • 45
    • 24144500631 scopus 로고    scopus 로고
    • Sterol structure determines miscibility versus melting transitions in lipid vesicles
    • Beattie ME, Veatch SL, Stottrup BL, Keller SL. Sterol structure determines miscibility versus melting transitions in lipid vesicles. Biophys J 2005, 89:1760-1768.
    • (2005) Biophys J , vol.89 , pp. 1760-1768
    • Beattie, M.E.1    Veatch, S.L.2    Stottrup, B.L.3    Keller, S.L.4
  • 46
    • 70350365111 scopus 로고    scopus 로고
    • Elegans major fats are stored in vesicles distinct from lysosome-related organelles.
    • O'Rourke EJ, Soukas AA, Carr CE, Ruvkun G C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab 2009, 10:430-435.
    • (2009) Cell Metab , vol.10 , pp. 430-435
    • O'Rourke, E.J.1    Soukas, A.A.2    Carr, C.E.3    Ruvkun, G.C.4
  • 48
    • 17444368686 scopus 로고    scopus 로고
    • Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells.
    • Wüstner D, Mondal M, Tabas I, Maxfield FR. Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells. Traffic 2005, 6:396-412.
    • (2005) Traffic , vol.6 , pp. 396-412
    • Wüstner, D.1    Mondal, M.2    Tabas, I.3    Maxfield, F.R.4
  • 50
    • 40449112030 scopus 로고    scopus 로고
    • Fluorescent detection of lipid droplets and associated proteins.
    • Listenberger LL, Brown DA. Fluorescent detection of lipid droplets and associated proteins. Curr Protoc Cell Biol 2007, 24.
    • (2007) Curr Protoc Cell Biol , vol.24
    • Listenberger, L.L.1    Brown, D.A.2
  • 51
    • 0023007574 scopus 로고
    • Microspectrofluorometric quantitation of autofluorescent lipopigment in the human sympathetic ganglia
    • Koistinaho J, Sorvaniemi M, Alho H, Hervonen A. Microspectrofluorometric quantitation of autofluorescent lipopigment in the human sympathetic ganglia. Mech Ageing Dev 1986, 37:79-89.
    • (1986) Mech Ageing Dev , vol.37 , pp. 79-89
    • Koistinaho, J.1    Sorvaniemi, M.2    Alho, H.3    Hervonen, A.4
  • 52
    • 26244468935 scopus 로고    scopus 로고
    • Visualization of glucocorticoid receptor in the brain of green fluorescent protein-glucocorticoid receptor knockin mice
    • Usuku T, Nishi M, Morimoto M, Brewer JA, Muglia LJ, Sugimoto T, Kawata M. Visualization of glucocorticoid receptor in the brain of green fluorescent protein-glucocorticoid receptor knockin mice. Neuroscience 2005, 135:1119-1128.
    • (2005) Neuroscience , vol.135 , pp. 1119-1128
    • Usuku, T.1    Nishi, M.2    Morimoto, M.3    Brewer, J.A.4    Muglia, L.J.5    Sugimoto, T.6    Kawata, M.7
  • 54
    • 0016063911 scopus 로고
    • The genetics of Caenorhabditis elegans
    • Brenner S. The genetics of Caenorhabditis elegans. Genetics 1974, 77:71-94.
    • (1974) Genetics , vol.77 , pp. 71-94
    • Brenner, S.1
  • 55
    • 0037119406 scopus 로고    scopus 로고
    • Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells.
    • Wüstner D, Herrmann A, Hao M, Maxfield FR. Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells. J Biol Chem 2002, 277:30325-30336.
    • (2002) J Biol Chem , vol.277 , pp. 30325-30336
    • Wüstner, D.1    Herrmann, A.2    Hao, M.3    Maxfield, F.R.4
  • 56
    • 0038725897 scopus 로고    scopus 로고
    • Genome-wide RNAi screening in Caenorhabditis elegans
    • Kamath RS, Ahringer RJ. Genome-wide RNAi screening in Caenorhabditis elegans. Methods 2003, 30:313-321.
    • (2003) Methods , vol.30 , pp. 313-321
    • Kamath, R.S.1    Ahringer, R.J.2
  • 57
    • 0024291340 scopus 로고
    • Dpy-13, a nematode collagen gene that affects body shape
    • von Mende N, Albert PS, Riddle DL, Bird DM. Dpy-13, a nematode collagen gene that affects body shape. Cell 1988, 55:567-576.
    • (1988) Cell , vol.55 , pp. 567-576
    • von Mende, N.1    Albert, P.S.2    Riddle, D.L.3    Bird, D.M.4
  • 58
    • 33846056461 scopus 로고    scopus 로고
    • Plasma membrane sterol distribution resembles the surface topography of living cells
    • Wüstner D. Plasma membrane sterol distribution resembles the surface topography of living cells. Mol Biol Cell 2007, 18:211-228.
    • (2007) Mol Biol Cell , vol.18 , pp. 211-228
    • Wüstner, D.1


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