Development and application of a real-time TaqMan® qPCR assay for detection and quantification of 'Candidatus Mycoplasma haemolamae' in South American camelids

Veterinary Microbiology

Volumn 146, Issue 3-4, 2010, Pages 290-294

  Meli, Marina L ^a   Kaufmann, Christine ^b   Zanolari, Patrik ^c   Robert, Nadia ^c   Willi, Barbara ^a   Lutz, Hans ^a   Hofmann Lehmann, Regina ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b Animal Clinic mondo a   (Switzerland)

^c VETSUISSE FACULTY UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

'Candidatus Mycoplasma haemolamae'; 16S rRNA gene; Alpaca; Hemotropic mycoplasmas; Llama; Molecular diagnostics; Real time TaqMan PCR

Indexed keywords

RNA 16S;

ARTICLE; ARTIODACTYLA; BACTERIAL COUNT; BACTERIUM DETECTION; CANDIDATUS MYCOPLASMA HAEMOLAMAE; CONTROLLED STUDY; DIAGNOSTIC KIT; EPERYTHROZOONOSIS; GENE AMPLIFICATION; HERD; MYCOPLASMA; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SWITZERLAND;

ANIMALS; CAMELIDS, NEW WORLD; FEMALE; MALE; MOLECULAR SEQUENCE DATA; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SWITZERLAND;

BACTERIA (MICROORGANISMS); CAMELIDAE; CANDIDATUS MYCOPLASMA HAEMOLAMAE; LAMA (MAMMAL); LAMA PACOS; MYCOPLASMA; MYCOPLASMATALES;

EID: 78650311122     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2010.05.029     Document Type: Article

References (30)

1. Almy F.S., Ladd S.M., Sponenberg D.P., Crisman M.V., Messick J.B. Mycoplasma haemolamae infection in a 4-day-old cria: support for in utero transmission by use of a polymerase chain reaction assay. Can. Vet. J. 2006, 47:229-233.
2. Criado-Fornelio A., Martinez-Marcos A., Buling-Sarana A., Barba-Carretero J.C. Presence of Mycoplasma haemofelis, Mycoplasma haemominutum and piroplasmids in cats from southern Europe: a molecular study. Vet. Microbiol. 2003, 93:307-317.
3. Foley J.E., Harrus S., Poland A., Chomel B., Pedersen N.C. Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats. Am. J. Vet. Res. 1998, 59:1581-1588.
4. Grindem C.B., Corbett W.T., Tomkins M.T. Risk factors for Haemobartonella felis infection in cats. J. Am. Vet. Med. Assoc. 1990, 196:96-99.
5. Hoelzle L.E., Helbling M., Hoelzle K., Ritzmann M., Heinritzi K., Wittenbrink M.M. First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples. J. Microbiol. Methods 2007, 70:346-354.
6. Hofmann-Lehmann R., Meli M.L., Dreher U.M., Gonczi E., Deplazes P., Braun U., Engels M., Schupbach J., Jorger K., Thoma R., Griot C., Stark K.D., Willi B., Schmidt J., Kocan K.M., Lutz H. Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland. J. Clin. Microbiol. 2004, 42:3775-3780.
7. Jensen W.A., Lappin M.R., Kamkar S., Reagan W.J. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats. Am. J. Vet. Res. 2001, 62:604-608.
8. Kaufmann C., Meli M.L., Robert N., Willi B., Hofmann-Lehmann R., Wengi N., Lutz H., Zanolari P. Haemotrophic mycoplasmas in South American camelids in Switzerland. Proceedings of the 43rd International Symposium on Diseases of Zoo and Wild Animals 2007, 13-17.
9. Kenny M.J., Shaw S.E., Beugnet F., Tasker S. Demonstration of two distinct hemotropic mycoplasmas in French dogs. J. Clin. Microbiol. 2004, 42:5397-5399.
10. Klein D., Janda P., Steinborn R., Muller M., Salmons B., Gunzburg W.H. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification. Electrophoresis 1999, 20:291-299.
11. Lascola K., Vandis M., Bain P., Bedenice D. Concurrent Infection with Anaplasma phagocytophilum and Mycoplasma haemolamae in a young alpaca. J. Vet. Intern. Med. 2009, 23:379-382.
12. Luria B.J., Levy J.K., Lappin M.R., Breitschwerdt E.B., Legendre A.M., Hernandez J.A., Gorman S.P., Lee I.T. Prevalence of infectious diseases in feral cats in Northern Florida. J. Feline Med. Surg. 2004, 6:287-296.
13. McLaughlin B.G., Evans C.N., McLaughlin P.S., Johnson L.W., Smith A.R., Zachary J.F. An Eperythrozoon-like parasite in llamas. J. Am. Vet. Med. Assoc. 1990, 197:1170-1175.
14. Messick J.B., Berent L.M., Cooper S.K. Development and evaluation of a PCR-based assay for detection of Haemobartonella felis in cats and differentiation of H. felis from related bacteria by restriction fragment length polymorphism analysis. J. Clin. Microbiol. 1998, 36:462-466.
15. Messick J.B., Walker P.G., Raphael W., Berent L., Shi X. 'Candidatus Mycoplasma haemodidelphidis' sp. nov., 'andidatus Mycoplasma haemolamae' sp. nov. and Mycoplasma haemocanis comb. nov., haemotrophic parasites from a naturally infected opossum (Didelphis virginiana), alpaca (Lama pacos) and dog (Canis familiaris): phylogenetic and secondary structural relatedness of their 16S rRNA genes to other mycoplasmas. Int. J. Syst. Evol. Microbiol. 2002, 52:693-698.
16. Messick J.B. New perspectives about hemotrophic mycoplasma (formerly Haemobartonella and Eperythrozoon species) infections in dogs and cats. Vet. Clin. North Am. Small Anim. Pract. 2003, 33:1453-1465.
17. Messick J.B. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet. Clin. Pathol. 2004, 33:2-13.
18. Peters I.R., Helps C.R., Willi B., Hofmann-Lehmann R., Tasker S. The prevalence of three species of feline haemoplasmas in samples submitted to a diagnostics service as determined by three novel real-time duplex PCR assays. Vet. Microbiol. 2008, 126:142-150.
19. Reagan W.J., Garry F., Thrall M.A., Colgan S., Hutchison J., Weiser M.G. The clinicopathologic, light, and scanning electron microscopic features of eperythrozoonosis in four naturally infected llamas. Vet. Pathol. 1990, 27:426-431.
20. Sykes J.E., Ball L.M., Bailiff N.L., Fry M.M. 'Candidatus Mycoplasma haematoparvum', a novel small haemotropic mycoplasma from a dog. Int. J. Syst. Evol. Microbiol. 2005, 55:27-30.
21. Tandon R., Cattori V., Gomes-Keller M.A., Meli M.L., Golder M.C., Lutz H., Hofmann-Lehmann R. Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction. J. Virol. Methods 2005, 130:124-132.
22. Tasker S., Binns S.H., Day M.J., Gruffydd-Jones T.J., Harbour D.A., Helps C.R., Jensen W.A., Olver C.S., Lappin M.R. Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in cats in the United Kingdom. Vet. Rec. 2003, 152:193-198.
23. Tasker S., Helps C.R., Day M.J., Gruffydd-Jones T.J., Harbour D.A. Use of real-time PCR to detect and quantify Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' DNA. J. Clin. Microbiol. 2003, 41:439-441.
24. Tornquist, S.J., 2002. The infection formerly known as eperythrozoonosis in Camelids: new information and new test, http://www.shagbarkridge.com/info/epe.html.
25. Tornquist S.J., Boeder L.J., Cebra C.K., Messick J. Use of a polymerase chain reaction assay to study response to oxytetracycline treatment in experimental 'Candidatus Mycoplasma haemolamae' infection in alpacas. Am. J. Vet. Res. 2009, 70:1102-1107.
26. Wengi N., Willi B., Boretti F.S., Cattori V., Riond B., Meli M.L., Reusch C.E., Lutz H., Hofmann-Lehmann R. Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas. Vet. Microbiol. 2008, 126:132-141.
27. Willi B., Boretti F.S., Cattori V., Tasker S., Meli M.L., Reusch C., Lutz H., Hofmann-Lehmann R. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland. J. Clin. Microbiol. 2005, 43:2581-2585.
28. Willi B., Boretti F.S., Baumgartner C., Tasker S., Wenger B., Cattori V., Meli M.L., Reusch C.E., Lutz H., Hofmann-Lehmann R. Prevalence, risk factor analysis, and follow-up of infections caused by three feline hemoplasma species in cats in Switzerland. J. Clin. Microbiol. 2006, 44:961-969.
29. Willi B., Boretti F.S., Meli M.L., Bernasconi M.V., Casati S., Hegglin D., Puorger M., Neimark H., Cattori V., Wengi N., Reusch C.E., Lutz H., Hofmann-Lehmann R. Real-time PCR investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas. Appl. Environ. Microbiol. 2007, 73:3798-3802.
30. Willi B., Meli M.L., Luthy R., Honegger H., Wengi N., Hoelzle L.E., Reusch C.E., Lutz H., Hofmann-Lehmann R. Development and application of a universal hemoplasma screening assay based on the SYBR green PCR principle. J. Clin. Microbiol. 2009, 47:4049-4054.