Development and application of a universal hemoplasma screening assay based on the SYBR green PCR principle

Journal of Clinical Microbiology

Volumn 47, Issue 12, 2009, Pages 4049-4054

  Willi, Barbara ^a   Meli, Marina L ^a   Lüthy, Ruedi ^b   Honegger, Hanspeter ^c   Wengi, Nicole ^a   Hoelzle, Ludwig E ^a   Reusch, Claudia E ^a   Lutz, Hans ^a   Hofmann Lehmann, Regina ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b Swiss Aids Care International   (Switzerland)

^c TRIEMLI HOSPITAL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; COMPARATIVE STUDY; DIAGNOSTIC ACCURACY; EPERYTHROZOONOSIS; GENE AMPLIFICATION; HUMAN; IXODES; MAJOR CLINICAL STUDY; MYCOPLASMA; MYCOPLASMA PENETRANS; MYCOPLASMA PNEUMONIAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING TEST; SENSITIVITY AND SPECIFICITY; SWITZERLAND;

ANIMALS; CAT DISEASES; CATS; CATTLE; DNA, BACTERIAL; DOGS; HUMANS; MASS SCREENING; MICE; MYCOPLASMA; MYCOPLASMA INFECTIONS; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SWITZERLAND; TAQ POLYMERASE;

BOVINAE; CAMELIDAE; FELIDAE; IXODES; IXODIDA; MAMMALIA; MURINAE; MYCOPLASMA PENETRANS; MYCOPLASMA PNEUMONIAE; MYCOPLASMATALES; SUS;

EID: 71549115764     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01478-09     Document Type: Article

References (37)

1. Aeschlimann, A. 1972. Ixodes ricinus, Limmeus, 1758 (Ixodoidea: Ixodidae). Preliminary study of the biology of the species in Switzerland. Acta Trop. 29:321-340.
2. Applied Biosystems. 2006. SYBR® Green PCR master mix and RT-PCR reagents, protocol. Applied Biosystems, Foster City, CA.
3. Archer, G. L., P. H. Coleman, R. M. Cole, R. J. Duma, and C. L. Johnston, Jr. 1980. Hemotropic bacteria. N. Engl. J. Med. 302:1151-1152.
4. Brunner, C., T. Kanellos, M. L. Meli, D. J. Sutton, R. Gisler, M. A. Gomes-Keller, R. Hofmann-Lehmann, and H. Lutz. 2006. Antibody induction after combined application of an adjuvanted recombinant FeLV vaccine and a multivalent modified live virus vaccine with a chlamydial component. Vaccine 24:1838-1846. (Pubitemid 43238265)
5. Criado-Fornelio, A., A. Martinez-Marcos, A. Buling-Sarana, and J. C. Barba-Carretero. 2003. Presence of Mycoplasma haemofelis, Mycoplasma haemominutum and piroplasmids in cats from southern Europe: a molecular study. Vet. Microbiol. 93:307-317.
6. dos Santos, A. P., R. P. dos Santos, A. W. Biondo, J. M. Dora, L. Z. Goldani, S. T. de Oliveira, A. M. de Sa Guimaraes, J. Timenetsky, H. A. de Morais, F. H. Gonzalez, and J. B. Messick. 2008. Hemoplasma infection in HIV-positive patient, Brazil. Emerg. Infect. Dis. 14:1922-1924.
7. Duarte, M. I., M. S. Oliveira, M. A. Shikanai-Yasuda, O. N. Mariano, C. F. Takakura, C. Pagliari, and C. E. Corbett. 1992. Haemobartonella-like microorganism infection in AIDS patients: ultrastructural pathology. J. Infect. Dis. 165:976-977.
8. Fyumagwa, R. D., S. Simmler, B. Willi, M. L. Meli, A. Sutter, R. Hoare, G. Dasen, R. Hofmann-Lehmann, and H. Lutz. 2007. Molecular detection of hemotropic mycoplasmas in Rhipicephalus sanguineus tick species collected on lions (Panthera leo) from Ngorongoro Crater, Tanzania. S. Afr. J. Wildl. Res. 38:117-122.
9. Giglio, S., P. T. Monis, and C. P. Saint. 2003. Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR. Nucleic Acids Res. 31:e136.
10. Grindem, C. B., W. T. Corbett, and M. T. Tomkins. 1990. Risk factors for Haemobartonella felis infection in cats. J. Am. Vet. Med. Assoc. 196:96-99.
11. Hoelzle, L. E., M. Helbling, K. Hoelzle, M. Ritzmann, K. Heinritzi, and M. M. Wittenbrink. 2007. First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples. J. Microbiol. Methods 70:346-354.
12. Inokuma, H., M. Oyamada, B. Davoust, M. Boni, J. Dereure, B. Bucheton, A. Hammad, M. Watanabe, K. Itamoto, M. Okuda, and P. Brouqui. 2006. Epidemiological survey of Ehrlichia canis and related species infection in dogs in eastern Sudan. Ann. N. Y. Acad. Sci. 1078:461-463.
13. Jensen, W. A., M. R. Lappin, S. Kamkar, and W. J. Reagan. 2001. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats. Am. J. Vet. Res. 62:604-608.
14. Kallick, C. A., S. Levin, K. T. Reddi, and W. L. Landau. 1972. Systemic lupus erythematosus associated with haemobartonella-like organisms. Nat. New Biol. 236:145-146.
15. Klein, D., P. Janda, R. Steinborn, M. Muller, B. Salmons, and W. H. Gunzburg. 1999. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification. Electrophoresis 20:291-299. (Pubitemid 29125016)
16. Luria, B. J., J. K. Levy, M. R. Lappin, E. B. Breitschwerdt, A. M. Legendre, J. A. Hernandez, S. P. Gorman, and I. T. Lee. 2004. Prevalence of infectious diseases in feral cats in Northern Florida. J. Feline Med. Surg. 6:287-296.
17. Messick, J. B. 2003. New perspectives about hemotrophic mycoplasma (formerly, Haemobartonella and Eperythrozoon species) infections in dogs and cats. Vet. Clin. N. Am. Small Anim. Pract. 33:1453-1465.
18. Messick, J. B., L. M. Berent, and S. K. Cooper. 1998. Development and evaluation of a PCR-based assay for detection of Haemobartonella felis in cats and differentiation of H. felis from related bacteria by restriction fragment length polymorphism analysis. J. Clin. Microbiol. 36:462-466.
19. Neimark, H., K. E. Johansson, Y. Rikihisa, and J. G. Tully. 2002. Revision of haemotrophic Mycoplasma species names. Int. J. Syst. Evol. Microbiol. 52:683.
20. Peters, I. R., C. R. Helps, B. Willi, R. Hofmann-Lehmann, and S. Tasker. 2008. The prevalence of three species of feline haemoplasmas in samples submitted to a diagnostics service as determined by three novel real-time duplex PCR assays. Vet. Microbiol. 126:142-150.
21. Rikihisa, Y., M. Kawahara, B. Wen, G. Kociba, P. Fuerst, F. Kawamori, C. Suto, S. Shibata, and M. Futohashi. 1997. Western immunoblot analysis of Haemobartonella muris and comparison of 16S rRNA gene sequences of H. muris, H. felis, and Eperythrozoon suis. J. Clin. Microbiol. 35:823-829. (Pubitemid 27146518)
22. Ristic, M., and J. P. Kreier. 1979. Hemotropic bacteria. N. Engl. J. Med. 301:937-939.
23. Stockham, S. L., and A. M. Scott. 2008. Fundamentals of veterinary clinical pathology, 2nd ed. Iowa State University Press, Ames, IA.
24. Sykes, J. E., N. L. Bailiff, L. M. Ball, O. Foreman, J. W. George, and M. M. Fry. 2004. Identification of a novel hemotropic mycoplasma in a splenectomized dog with hemic neoplasia. J. Am. Vet. Med. Assoc. 224:1946-1951, 1930-1931.
25. Tasker, S., S. H. Binns, M. J. Day, T. J. Gruffydd-Jones, D. A. Harbour, C. R. Helps, W. A. Jensen, C. S. Olver, and M. R. Lappin. 2003. Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in cats in the United Kingdom. Vet. Rec. 152:193-198.
26. Tasker, S., S. M. Caney, M. J. Day, R. S. Dean, C. R. Helps, T. G. Knowles, P. J. Lait, M. D. Pinches, and T. J. Gruffydd-Jones. 2006. Effect of chronic FIV infection, and efficacy of marbofloxacin treatment, on Mycoplasma haemofelis infection. Vet. Microbiol. 117:169-179.
27. Tasker, S., C. R. Helps, C. J. Belford, R. J. Birtles, M. J. Day, A. H. Sparkes, T. J. Gruffydd-Jones, and D. A. Harbour. 2001. 16S rDNA comparison demonstrates near identity between an United Kingdom Haemobartonella felis strain and the American California strain. Vet. Microbiol. 81:73-78.
28. Tasker, S., C. R. Helps, M. J. Day, D. A. Harbour, S. E. Shaw, S. Harrus, G. Baneth, R. G. Lobetti, R. Malik, J. P. Beaufils, C. R. Belford, and T. J. Gruffydd-Jones. 2003. Phylogenetic analysis of hemoplasma species: an international study. J. Clin. Microbiol. 41:3877-3880.
29. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
30. Wengi, N., B. Willi, F. S. Boretti, V. Cattori, B. Riond, M. L. Meli, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2008. Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas. Vet. Microbiol. 126:132-141.
31. Wicki, R., P. Sauter, C. Mettler, A. Natsch, T. Enzler, N. Pusterla, P. Kuhnert, G. Egli, M. Bernasconi, R. Lienhard, H. Lutz, and C. M. Leutenegger. 2000. Swiss Army survey in Switzerland to determine the prevalence of Francisella tularensis, members of the Ehrlichia phagocytophila genogroup, Borrelia burgdorferi sensu lato, and tick-borne encephalitis virus in ticks. Eur. J. Clin. Microbiol. Infect. Dis. 19:427-432.
32. Willi, B., F. S. Boretti, C. Baumgartner, S. Tasker, B. Wenger, V. Cattori, M. L. Meli, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2006. Prevalence, risk factor analysis, and follow-up of infections caused by three feline hemoplasma species in cats in Switzerland. J. Clin. Microbiol. 44:961-969.
33. Willi, B., F. S. Boretti, V. Cattori, S. Tasker, M. L. Meli, C. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2005. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland. J. Clin. Microbiol. 43:2581-2585.
34. Willi, B., F. S. Boretti, M. L. Meli, M. V. Bernasconi, S. Casati, D. Hegglin, M. Puorger, H. Neimark, V. Cattori, N. Wengi, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2007. Real-time PCR investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas. Appl. Environ. Microbiol. 73:3798-3802.
35. Willi, B., C. Filoni, J. L. Catao-Dias, V. Cattori, M. L. Meli, A. Vargas, F. Martinez, M. E. Roelke, M. P. Ryser-Degiorgis, C. M. Leutenegger, H. Lutz, and R. Hofmann-Lehmann. 2007. Worldwide occurrence of feline hemoplasma infections in wild felid species. J. Clin. Microbiol. 45:1159-1166.
36. Willi, B., S. Tasker, F. S. Boretti, M. G. Doherr, V. Cattori, M. L. Meli, R. G. Lobetti, R. Malik, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2006. Phylogenetic analysis of "Candidatus Mycoplasma turicensis" isolates from pet cats in the United Kingdom, Australia, and South Africa, with analysis of risk factors for infection. J. Clin. Microbiol. 44:4430-4435.
37. Yang, D., X. Tai, Y. Qiu, and S. Yun. 2000. Prevalence of Eperythrozoon spp. infection and congenital eperythrozoonosis in humans in Inner Mongolia, China. Epidemiol. Infect. 125:421-426.