Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA

Journal of Clinical Microbiology

Volumn 41, Issue 1, 2003, Pages 439-441

  Tasker, Séverine ^a   Helps, Chris R ^a   Day, Michael J ^a   Gruffydd Jones, Tim J ^a   Harbour, Dave A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; TAQ POLYMERASE;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL COUNT; BACTERIAL INFECTION; BACTERIAL KINETICS; BACTERIUM DETECTION; CANDIDATUS MYCOPLASMA HAEMOMINUTUM; CAT; CONTROLLED STUDY; DNA PROBE; FEMALE; MYCOPLASMA; MYCOPLASMA HAEMOFELIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

ANIMALS; CAT DISEASES; CATS; DISEASE MODELS, ANIMAL; DNA, BACTERIAL; KINETICS; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BACTERIA (MICROORGANISMS); CANDIDATUS; CANDIDATUS MYCOPLASMA HAEMOMINUTUM; FELIDAE; FELIS CATUS; MYCOPLASMA; MYCOPLASMA HAEMOFELIS;

EID: 0037232120     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.1.439-441.2003     Document Type: Article

References (9)

1. Amikam, D., G. Glaser, and S. Razin. 1984. Mycoplasmas (Mollicutes) have a low number of rRNA genes. J. Bacteriol. 158:376-378.
2. Bustin, S. A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:169-193.
3. Foley, J. E., S. Harrus, A. Poland, B. Chomel, and N. C. Pedersen. 1998. Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats. Am. J. Vet. Res. 59:1581-1588.
4. Foley, J. E., and N. C. Pedersen. 2001. 'Candidatus Mycoplasma haemominutum', a low-virulence epierythrocytic parasite of cats. Int. J. Syst. Evol. Microbiol. 51:815-817.
5. Jensen, W. A., M. R. Lappin, S. Kamkar, and W. J. Reagan. 2001. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis infection in naturally infected cats. Am. J. Vet. Res. 62:604-608.
6. Nelmark, H., K. E. Johansson, Y. Rikihisa, and J. G. Tully. 2001. Proposal to transfer some members of the genera Haemobartonella and Eperythrozoon to the genus Mycoplasma with descriptions of 'Candidatus Mycoplasma haemofelis', 'Candidatus Mycoplasma haemomuris', 'Candidatus Mycoplasma haemosuis' and 'Candidatus Mycoplasma wenyonii'. Int. J. Syst. Evol. Microbiol. 51:891-899.
7. Neimark, H., K. E. Johansson, Y. Rikihisa, and J. G. Tully. 2002. Revision of haemotrophic Mycoplasma species names. Int. J. Syst. Evol. Microbiol. 52:683.
8. Tasker, S., S. H. Binns, M. J. Day, T. J. Gruffydd-Jones, D. A. Harbour, C. R. Helps, W. A. Jensen, C. S. Olver, and M. R. Lappin. Use of a polymerase chain reaction assay to assess prevalence and risk factors for Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in the United Kingdom. Vet. Rec., in press.
9. Westfall, D. S., W. A. Jensen, W. J. Reagan, S. V. Radecki, and M. R. Lappin. 2001. Inoculation of two genotypes of Hemobartonella felis (California and Ohio variants) to induce infection in cats and the response to treatment with azithromycin. Am. J. Vet. Res. 62:687-691.