Prokaryotic expression, refolding, and purification of functional human vascular endothelial growth factor isoform 165: Purification procedures and refolding conditions revisited

Protein Expression and Purification

Volumn 76, Issue 1, 2011, Pages 54-58

  Lee, I Liang ^a   Li, Pei Shan ^b   Yu, Wei Lin ^b   Shen, Hsin Hsin ^b  

^a NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

^b INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

Author keywords

Angiogenesis; Expression; Purification; Recombinant VEGF protein; Refolding; Untagged protein

Indexed keywords

RECOMBINANT PROTEIN; VASCULOTROPIN A; VEGFA PROTEIN, HUMAN;

ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; HUMAN; ISOLATION AND PURIFICATION; PROTEIN REFOLDING;

ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; PROTEIN REFOLDING; RECOMBINANT PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 78650179837     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.014     Document Type: Article

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