Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays

Analytical and Bioanalytical Chemistry

Volumn 398, Issue 7-8, 2010, Pages 3023-3032

  Heus, Ferry ^a   Giera, Martin ^a   De Kloe, Gerdien E ^a   Van Iperen, Dick ^a   Buijs, Joost ^a   Nahar, Tariq T ^a   Smit, August B ^a   Lingeman, Henk ^a   De Esch, Iwan J P ^a   Niessen, Wilfried M A ^a   Irth, Hubertus ^a   Kool, Jeroen ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Acetylcholine binding protein; Bioassay; LED based confocal fluorescence detection; Microfluidics; Miniaturization

Indexed keywords

ACETYLCHOLINE-BINDING PROTEINS; BIOACTIVE COMPOUNDS; BIOCHEMICAL ASSAY; BIOCHEMICAL DETECTION; BUBBLE CELLS; CARRIER FLOW; COMPLEX MIXTURE; CONFOCAL FLUORESCENCE; DETECTION CELLS; DETECTION SYSTEM; EXTRACELLULAR LIGANDS; FLOW INJECTION ANALYSIS; FLUORESCENCE DETECTION SYSTEM; INJECTION VOLUME; LIGHT PATH; MINIATURIZATION; NANO-LC; NICOTINIC ACETYLCHOLINE RECEPTORS; REAGENT CONSUMPTION; RECEPTOR PROTEINS; REVERSED PHASE; SCREENING APPROACHES; VOID VOLUME;

FLUORESCENCE; LIGANDS; LIGHT EMITTING DIODES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MICROFLUIDICS; MINIATURE INSTRUMENTS; MIXTURES; PROTEINS;

BIOASSAY;

ACHBP PROTEIN, LYMNAEA; CARRIER PROTEIN;

ARTICLE; BIOASSAY; FLOW INJECTION ANALYSIS; FLUORESCENCE MICROSCOPY; INSTRUMENTATION; LIQUID CHROMATOGRAPHY; METHODOLOGY; MICROFLUIDICS;

BIOLOGICAL ASSAY; CARRIER PROTEINS; CHROMATOGRAPHY, LIQUID; FLOW INJECTION ANALYSIS; MICROFLUIDICS; MICROSCOPY, FLUORESCENCE;

EID: 78650173376     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-4210-x     Document Type: Article

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