An automated high-content assay for tumor cell migration through 3-Dimensional matrices

Journal of Biomolecular Screening

Volumn 15, Issue 9, 2010, Pages 1144-1151

  Echeverria, Victoria ^a   Meyvantsson, Ivar ^a   Skoien, Allyson ^a   Worzella, Tracy ^a   Lamers, Casey ^a   Hayes, Steven ^a  

^a Bellbrook Labs LLC   (United States)

Author keywords

high content analysis; invasion; migration assay; tumor cell

Indexed keywords

ARTICLE; ASSAY; AUTOMATION; CELL MIGRATION; CELL STRUCTURE; CONTROLLED STUDY; DEVICE; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; IMAGING SYSTEM; INHIBITION KINETICS; METASTASIS; PRIORITY JOURNAL; ROBOTICS; THREE DIMENSIONAL IMAGING; TUMOR CELL;

AUTOMATION; CELL COUNT; CELL LINE, TUMOR; CELL MIGRATION ASSAYS; CELL MOVEMENT; COLLAGEN; EXTRACELLULAR MATRIX; HUMANS; LAMININ; NEOPLASM INVASIVENESS; NEOPLASMS;

EID: 78650162975     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110378890     Document Type: Article

References (17)

1. Wolf K., Mazo I., Leung H., Engelke K., von Andrian UH, Deryugina EI, et al. Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis. J Cell Biol 2003 ; 160 (2). 267-277.
2. Sahai E., Marshall CJ: Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis. Nat Cell Biol 2003 ; 5 (8). 711-719.
3. Carragher NO: Profiling distinct mechanisms of tumour invasion for drug discovery: imaging adhesion, signalling and matrix turnover. Clin Exp Metastasis 2009 ; 26 (4). 381-397.
4. Daley WP, Peters SB, Larsen M.: Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 2008 ; 121 (pt 3). 255-264.
5. Roskelley CD, Bissell MJ: Dynamic reciprocity revisited: a continuous, bidirectional flow of information between cells and the extracellular matrix regulates mammary epithelial cell function. Biochem Cell Biol 1995 ; 73 (7-8). 391-397.
6. Hansen RK, Bissell MJ: Tissue architecture and breast cancer: the role of extracellular matrix and steroid hormones. Endocr Relat Cancer 2000 ; 7 (2). 95-113.
7. Kozlowski JM, Fidler IJ, Campbell D., Xu ZL, Kaighn ME, Hart IR: Metastatic behavior of human tumor cell lines grown in the nude mouse. Cancer Res 1984 ; 44 (8). 3522-3529.
8. Xu L., Bergan RC: Genistein inhibits matrix metalloproteinase type 2 activation and prostate cancer cell invasion by blocking the transforming growth factor beta-mediated activation of mitogen-activated protein kinase-activated protein kinase 2-27-kDa heat shock protein pathway. Mol Pharmacol 2006 ; 70 (3). 869-877.
9. BD Biosciences: Product specification sheet: rat tail collagen high concentration (HC), type 1. http://www.bdbiosciences.com/external-files/dl/doc/ manuals/live/web-enabled/354249-pug.pdf.
10. Walker GM, Beebe DJ: A passive pumping method for microfluidic devices. Lab Chip 2002 ; 2 (3). 131-134.
11. Meyvantsson I., Warrick JW, Hayes S., Skoien A., Beebe DJ: Automated cell culture in high density tubeless microfluidic device arrays. Lab Chip 2008 ; 8 (5). 717-724.
12. Echeverria V., Meyvantsson I., Hayes S.: Tumor cell migration assays in 3-D ECM. Genetic Engineering News 2010 ; 30 (5). 34-35.
13. Cooper JA: Effects of cytochalasin and phalloidin on actin. J Cell Biol 1987 ; 105 (4). 1473-1478.
14. Coue M., Brenner SL, Spector I., Korn ED: Inhibition of actin polymerization by latrunculin A. FEBS Lett 1987 ; 213 (2). 316-318.
15. Straight AF, Cheung A., Limouze J., Chen I., Westwood NJ, Sellers JR, et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003 ; 299 (5613). 1743-1747.
16. Niggemann B., Drell TL IV, Joseph J., Weidt C., Lang K., Zaenker KS, et al. Tumor cell locomotion: differential dynamics of spontaneous and induced migration in a 3D collagen matrix. Exp Cell Res 2004 ; 298 (1). 178-187.
17. Friedl P., Wolf K.: Tumour-cell invasion and migration: diversity and escape mechanisms. Nat Rev Cancer 2003 ; 3 (5). 362-374.