Development and application of a screening assay for glycoside phosphorylases

Analytical Biochemistry

Volumn 401, Issue 1, 2010, Pages 162-167

  De Groeve, M R M ^a   Tran, G H ^a   Van Hoorebeke, A ^a   Stout, J ^a   Desmet, T ^a   Savvides, S N ^a   Soetaert, W ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Cellobiosides; Glycoside phosphorylase; High throughput screening; Inorganic phosphate determination; Mutagenesis

Indexed keywords

BIOCHEMICAL ENGINEERING; ENZYMES; ESCHERICHIA COLI; MOLECULES; MOLYBDENUM COMPOUNDS; MUTAGENESIS; PHOSPHORYLATION; SUGARS;

ACCEPTOR SPECIFICITIES; CELLOBIOSE PHOSPHORYLASE; CELLOBIOSIDES; DEVELOPMENT AND APPLICATIONS; ESCHERICHIA COLI CELLS; HIGH THROUGHPUT SCREENING; INORGANIC PHOSPHATES; SATURATION MUTAGENESIS;

GLYCOSIDES;

CELLOBIOSE PHOSPHORYLASE; CYSTEINE; GLUTAMIC ACID; GLYCOSIDE; GLYCOSIDE PHOSPHORYLASE; GLYCOSYLTRANSFERASE; MOLYBDIC ACID; PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; CARBOHYDRATE SYNTHESIS; CELLULOMONAS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SPECIFICITY; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN VARIANT;

CELLULOMONAS UDA; ESCHERICHIA COLI;

EID: 77951094229     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.02.028     Document Type: Article

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