Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells and cell line

Human and Experimental Toxicology

Volumn 29, Issue 10, 2010, Pages 833-843

  Upreti, Raj K ^a   Kannan, A ^a   Pant, A B ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

Author keywords

acetyl salicylate; aspirin; gut bacteria; IEC 6 cell line; intestinal epithelial cells

Indexed keywords

ACETYLSALICYLIC ACID; CARRIER PROTEIN; ESTERASE; MEMBRANE ENZYME; NONSTEROID ANTIINFLAMMATORY AGENT; OXIDOREDUCTASE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BACTERIAL GROWTH; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CELL ENERGY; CELL RESPIRATION; CELL STRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG EFFECT; DRUG EXPOSURE; DRUG SCREENING; ENZYME ACTIVITY; ESCHERICHIA COLI; GASTROINTESTINAL TOXICITY; IN VITRO STUDY; INTESTINE EPITHELIUM CELL; INTESTINE FLORA; LACTOBACILLUS; MALE; MEMBRANE DAMAGE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; RAT; STAPHYLOCOCCUS; TOXICITY TESTING;

ANIMALS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; ASPIRIN; CELL LINE; CELL MEMBRANE; CELL SURVIVAL; COLONY COUNT, MICROBIAL; EPITHELIAL CELLS; ESTERASES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; IMAGE PROCESSING, COMPUTER-ASSISTED; INTESTINAL MUCOSA; INTESTINE, SMALL; MALE; MICROBIAL SENSITIVITY TESTS; OXIDOREDUCTASES; RATS; RATS, WISTAR;

RATTUS;

EID: 78649950691     PISSN: 09603271     EISSN: 14770903     Source Type: Journal    
DOI: 10.1177/0960327110363333     Document Type: Article

References (38)

1. Roth SH NSAID gastropathy: a new understanding. Arch Int Med 1996 ; 156: 1623-1628.
2. Wallace JL Building a better aspirin: gaseous solutions to a century-old problem. Brit J Pharmacol 2007 ; 152: 421-428.
3. Satoh H., Guth PH, Grossman MI Role of bacteria in gastric ulceration produced by indomethacin in the rat: cytoprotective actionof antibiotics. Gastroenterology 1983 ; 84: 483-489.
4. Price Ctd, Lee IR, Gustafson JE The effect of salicylate on bacteria. Int J Biochem Cell Biol 2000 ; 32: 1029-1043.
5. Basivireddy J., Jacob M., Ramamoorty P., Balasubramanian KA Alterations in the intestinal glycocalyx and bacterial flora in response to oral indomethacin. Int J Biochem Cell Biol 2005 ; 37: 2321-2332.
6. Tomisato W., et al. NSAIDs induce both necrosis and apoptosis in guinea pig gastric mucosal cells in primary culture. Am J Physiol Gastrointest Liver Physiol 2001 ; 281: G1092 - G1100.
7. Redlak MJ, Power JJ, Miller TA Role of mitochondria in aspirin-induced apoptosis in human gastric epithelial cells. Am J Physiol Gastrointest Liver Physiol 2005 ; 289: G731 - G738.
8. MacGowan A., Rogers C., Bowker K. In vitro models, in vivo models, and pharmacokinetics: what can we learn from in vitro models? Clin Infect Dis 2001 ; 15: S214 - S220.
9. Upreti RK, Shrivastava R., Kannan A., Chaturvedi UC A comparative study on rat intestinal epithelial cells and resident gut bacteria (i) Effect of hexavalent chromium. Toxicol Mech Methods 2005 ; 15: 331-338.
10. Upreti RK, Kannan A., Shrivastava R., Chaturvedi UC A comparative study on rat intestinal epithelial cells and resident gut bacteria (ii) Effect of arsenite. Biomed Environ Sci 2006 ; 19: 77-86.
11. Upreti RK, Kannan A., Pant AB Experimental exposure of arsenic in cultured rat intestinal epithelial cells and cell line: Toxicological consequences. Toxicol in Vitro 2007 ; 21: 32-40.
12. Espigares M., Mariscan A. The effect of 2,4-dichlorophenol on growth and plasmidic B-lactamase activity in E. coli. J Appl Toxicol 1989 ; 9: 427-431.
13. Forstner GG, Sabesin SM, Isselbacher KJ Rat intestinal microvillus membrane. Biochem J 1968 ; 106: 381-390.
14. Kumar M., Upreti RK Impact of lead stress and adaptation in E.coli. Ecotoxicol Environ Safety 2000 ; 47: 246-252.
15. Liu D. Effect of bacterial cultures on microbial toxicity assessment. Bull Environm Contam Toxicol 1985 ; 34: 331-339.
16. Obst U., Holzapfel-Pschorn A. Biochemical testing of ground water. Water Sci Technol 1988 ; 20: 101-107.
17. Weiser MM Intestinal epithelial cell surface membrane glycoprotein synthesis- An indicator of cellular differentiation. J Biol Chem 1973 ; 248: 2536-2541.
18. Hidalgo C., Gonzalez ME, Logos R. Characterization of the Ca2+- or Mg2+-ATPase of the transverse tubule membranes isolated from rabbit skeletal muscle. J Biol Chem 1983 ; 258: 13937-13945.
19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ Protein measurement with the folin phenol reagent. J Biol Chem 1951 ; 193: 265-275.
20. Roe JH The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem 1955 ; 212: 335-343.
21. Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959 ; 234: 1971-1975.
22. Folch J., Less M., Stanley GH A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957 ; 226: 497-509.
23. Wagner A., Lissau A., Holzl J., Horhammer L. The incorporation of P32 into the inositol phosphatides of rat brain. J Lipid Res 1962 ; 3: 177-180.
24. Zlatkis A., Zak B., Boyle AJ A new method for the direct determination of serum cholesterol. J Lab Clin Med 1953 ; 41: 486-492.
25. Shiff SJ, Koutsos MI, Qiao I., Rigas B. Nonsteroidal anti-inflammatory drugs inhibit the proliferation of colon adenocarcinoma cells: effect on cell cycle and apoptosis. Exp Cell Res 1996 ; 222: 179-188. (Pubitemid 26043724)
26. Ricchi, P., et al. Effect of aspirin on cell proliferation and differentiation of colon adenocarcinoma Caco-2 cells. Int J Cancer 1997 ; 73: 880-884.
27. Torslov J. Comparison of bacterial toxicity tests based on growth, dehydrogenase activity, and esterase activity of Pseudomonas fluorescen. Ecotoxicol Environm Safety 1993 ; 5: 33-40.
28. Roderick PJ, Wilkes HC, Meade TW The gastrointestinal toxicity of aspirin: an overview of randomized controlled trials. Br J Clin Pharmacol 1993 ; 35: 219-226.
29. Tomoda T., Takeda K., Kurashige T. Acetylsalicylic acid (ASA)-induced mitochondrial dysfunction and its potentiation by Ca2+. Liver 1994 ; 14: 103-108.
30. Khokher MA, Dandona PD The effect of indomethacin and aspirin on alkaline phosphatase secretion and [3H] thymidine incorporation by human osteoblasts. Rheumatology 1988 ; 27: 291-294.
31. McAlindon ME, Muller AF, Filipowicz Hawkey CJ Effect of allopurinol, sulphasalzine, and vitamin C on aspirin induced gastroduodenal injury in human volunteers. Gut 1996 ; 38: 518-524.
32. Basivireddy J., et al. Indomethacin-induced free radical mediated changes in the intestinal brush border membranes. Biochem Pharmacol 2003 ; 65: 683-695.
33. Hooper LV, et al. Molecular analysis of commensal host-microbial relationship in the intestine. Science 2001 ; 291: 881-884.
34. Neish AS The gut microflora and intestinal epithelial cells: a continuing dialogue. Microbes Infec 2002 ; 4: 309-317.
35. Rumble RH, Roberts MS Determination of aspirin and its major metabolites in plasma by high-performance liquid chromatography without solvent extraction. J Chromatogr 1981 ; 225: 252-260.
36. Zucco F. Freshly isolated cells and cell lines from the intestine as an in vitro model for toxicological studies. Toxicol in Vitro 1993 ; 7: 397-402.
37. Sambruy Y., Ferruzza S., Ranaldi G., De Angelis I. Intestinal cell culture models: applications in toxicology and pharmacology. Cell Biol Toxicol 2001 ; 17: 301-317.
38. Upreti RK, Kannan A., Pant AB Alterations in rat gut bacteria and intestinal epithelial cells following experimental exposure of antimicrobials. FEMS Immunol Med Microbiol 2008 ; 54: 60-69.