Accumulation of Cytoplasmic Lipid Droplets in Bovine Embryos and Cryotolerance of Embryos Developed in Different Culture Systems Using Serum-Free or Serum-Containing Media

Molecular Reproduction and Development

Volumn 61, Issue 1, 2002, Pages 57-66

  Abe, Hiroyuki ^a,b   Yamashita, Shoko ^a   Satoh, Takeshi ^a   Hoshi, Hiroyoshi ^a,b  

^a Res Inst for the Funct Peptides   (Japan)

^b YAMAGATA UNIVERSITY   (Japan)

Author keywords

Cow; Cryopreservation; Histochemistry; Serum free culture; Ultrastructure

Indexed keywords

BOVINAE;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CRYOPRESERVATION; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; CYTOPLASM; DRUG EFFECT; ELECTRON MICROSCOPY; EMBRYO; EMBRYO DEATH; FERTILIZATION IN VITRO; FREEZING; LIPID METABOLISM; METABOLISM; METHODOLOGY; ULTRASTRUCTURE;

ANIMALS; CATTLE; CRYOPRESERVATION; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; CULTURE TECHNIQUES; CYTOPLASM; EMBRYO; EMBRYO LOSS; FERTILIZATION IN VITRO; FREEZING; LIPID METABOLISM; MICROSCOPY, ELECTRON;

EID: 0036371304     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrd.1131     Document Type: Article

References (34)

1. Abe H, Otoi T, Tachikawa S, Yamashita S, Satoh T, Hoshi H. 1999a. Fine structure of bovine morulae and blastocysts in vivo and in vitro. Anat Embryol 199:519-527.
2. Abe H, Yamashita S, Itoh T, Satoh T, Hoshi H. 1999b. Ultrastructure of bovine embryos developed from in vitro-matured and -fertilized oocytes: comparative morphological evaluation of embryos cultured either in serum-free or in serum-supplemented medium. Mol Reprod Dev 53:325-335.
3. Albihn A, Rodriguez-Martinez H, Gustafsson H. 1990. Morphology of Day 7 bovine demiembryos during in vitro reorganization. Acta Anat (Basel) 138:42-49.
4. Brackett BG, Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol Reprod 12:260-274.
5. Brackett BG, Oh YK, Evans JF, Donawick WJ. 1980. Fertilization and early development of cow ova. Biol Reprod 23:189-205.
6. Brinster RL. 1967. Carbon dioxide production from glucose by the preimplantation mouse embryo Exp Cell Res 47:271-277.
7. Dorland M, Gardner DK, Trounson AO. 1994. Serum in synthetic oviduct fluid causes mitochondrial degeneration in ovine embryos. J Reprod Fertil (Abstr Ser) 13:25.
8. Fawcett DW. 1981. Lipid. In: Fawcett DW, editor. The Cell. Philadelphia: W.B Saunders Company, p 655-667.
9. Ferguson EM, Leese HJ. 1999. Triglyceride content of bovine oocytes and early embryos J Reprod Fertil 116:373-378.
10. Fukuda Y, Ichikawa M, Naito K, Toyoda Y. 1990. Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage. Biol Reprod 42:114-119.
11. Gardner DK. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol Int 18:1163-1178.
12. Gardner DK, Lane M, Spitzer A, Batt PA. 1994. Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Reprod 50:390-400.
13. Greve T, Avery B, Callesen H. 1993. Viability of in-vivo and in-vitro produced bovine embryos. Reprod Dom Anim 28:164-169.
14. Hasler JF, Henderson WB, Hurtgen PJ, Jin ZQ, McCauley AD, Mower SA, Neely B, Shuey LS, Stokes JE, Trimmer SA. 1995. Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology 43:141-152.
15. Holm P, Walker SK, Seamark RF. 1996. Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo. J Reprod Fertil 107:173-181.
16. Leibo SP, Loskutoff NM. 1993. Cryobiology of in vitro-derived bovine embryos. Theriogenology 39:81-94.
17. Leibo SP, Pollard JW, Martino PA. 1995. Chilling and freezing sensitivity of 'reassembled' in vitro-derived bovine embryos. Theriogenology (Abstr) 43:265.
18. Massip A, Mermillod P, Dinnyes A. 1995. Morphology and biochemistry of in vitro produced bovine embryos: implications for their cryopreservation. Hum Reprod 10:3004-3011.
19. Maurer HR. 1992. Towards serum-free, chemically defined media for mammalian cell culture. In: Freshney RI, editor. Animal cell culture: a practical approach. Oxford: Oxford University Press. p 15-46.
20. Mills RM, Brinster RL. 1967. Oxygen consumption of preimplantation mouse embryos. Exp Cell Res 47:337-344.
21. Mohr LR, Trounson AO. 1981. Structural changes associated with freezing of bovine embryos. Biol Reprod 25:1009-1025.
22. Pinyopummintr T, Bavister BD. 1991. In vitro-matured/in vitro-fertilized bovine oocytes can develop into morulae/blastocysts in chemically defined protein-free culture media. Biol Reprod 45:736-742.
23. Plante L, King WA. 1994. Light and electron microscopic analysis of bovine embryos derived by in vitro and in vivo fertilization. J Assisted Reprod Genet 11:515-529.
24. Pollard JW, Leibo SP. 1993. Comparative cryobiology of in vitro and in vivo derived bovine embryos. Theriogenology (Abstr) 39:287.
25. Sata R, Tsujii H, Abe H, Yamashita S, Hoshi H. 1999. Fatty acid composition of bovine embryos cultured in serum-free and serum-containing medium during early embryonic developemnt. J Reprod Dev 45:97-103.
26. Shamsuddin M, Larsson B, Gustafsson H, Rodriguez-Martinez H. 1993. In vitro development up to hatching of bovine in vitro-matured and fertilized oocytes with or without support from somatic cells. Theriogenology 39:1067-1079.
27. Sinclair KD, McEvoy TG, Carolan C, Maxfield EK, Maltin CA, Young LE, Wilmut I, Robinson JJ, Broadbent PJ. 1998. Conceptus growth and development following in vitro culture of ovine embryos in media supplemented with bovine sera. Theriogenology (Abstr) 49:218.
28. Spector AA. 1973. Fatty acid, glyceride, and phospholipid metabolism. Growth Nutr Metabol Cells Cult 1:257-296.
29. Takagi M, Otoi T, Boediono A, Saha S, Suzuki T. 1994. Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Theriogenology 41:915-921.
30. Thompson JG, Gardner DK, Pugh PA, McMillan WH, Tervit HR. 1995. Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos. Biol Reprod 53:1385-1391.
31. Ushjjima H, Yamakawa H, Nagashima H. 1999. Cryopreservation of bovine pre-morula-stage in vitro matured/in vitro fertilized embryos after delipidation and before use in nucleus transfer. Biol Reprod 60:535-539.
32. Van Langendonckt A, Donnay I, Schuurbiers N, Auquier P, Carolan C, Massip A, Dessy F. 1997. Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium. J Reprod Fertil 109:87-93.
33. Yamashita S, Abe H, Itoh T, Satoh T, Hoshi H. 1999. A serum-free culture system for efficient in vitro production of bovine blastocysts and their improved viability after freezing and thawing. Cytotechnology 31:1-9.
34. Zhang L, Barry DM, Denniston RS, Bunch TD, Godke RA. 1993. Birth of live calves after transfer of frozen-thawed bovine embryos fertilized in vitro. Vet Rec 132:247-279.