Dual selection mechanisms drive efficient single-gene reverse genetics for rotavirus

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 43, 2010, Pages 18652-18657

  Trask, Shane D ^a   Taraporewala, Zenobia F ^a,c   Boehme, Karl W ^b   Dermody, Terence S ^b   Patton, John T ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

NSP2; Recombinant virus; Reoviridae; RNAi; Temperature sensitive virus

Indexed keywords

MESSENGER RNA; PROTEIN NSP2; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; CHIMERA; GENETIC ENGINEERING; GENETIC SELECTION; NONHUMAN; PRIORITY JOURNAL; RNA DEGRADATION; RNA INTERFERENCE; ROTAVIRUS; TEMPERATURE SENSITIVE MUTANT; VIRUS GENE; VIRUS MUTANT; VIRUS PLAQUE; VIRUS RECOMBINANT;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL LINE; DNA, VIRAL; GENES, VIRAL; GENETIC ENGINEERING; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; RNA INTERFERENCE; RNA-BINDING PROTEINS; ROTAVIRUS; SELECTION, GENETIC; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; TEMPERATURE; VIRAL NONSTRUCTURAL PROTEINS;

REOVIRIDAE; ROTAVIRUS;

EID: 78649809519     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1011948107     Document Type: Article

References (37)

1. Boyce M, Celma CC, Roy P (2008) Development of reverse genetics systems for bluetongue virus: Recovery of infectious virus from synthetic RNA transcripts. J Virol 82:8339-8348.
2. Kobayashi T, et al. (2007) A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Cell Host Microbe 1:147-157.
3. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS (2010) An improved reverse genetics system for mammalian orthoreoviruses. Virology 398:194-200.
4. Matsuo E, Roy P (2009) Bluetongue virus VP6 acts early in the replication cycle and can form the basis of chimeric virus formation. J Virol 83:8842-8848.
5. Matsuo E, Celma CC, Roy P (2010) A reverse genetics system of African horse sickness virus reveals existence of primary replication. FEBS Lett 584:3386-3391.
6. Komoto S, Sasaki J, Taniguchi K (2006) Reverse genetics system for introduction of site-specific mutations into the double-stranded RNA genome of infectious rotavirus. Proc Natl Acad Sci USA 103:4646-4651.
7. Komoto S, Kugita M, Sasaki J, Taniguchi K (2008) Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4. J Virol 82:6753-6757.
8. Troupin C, et al. (2010) Rearranged genomic RNA segments offer a new approach to the reverse genetics of rotaviruses. J Virol 84:6711-6719.
9. Taraporewala ZF, et al. (2006) Structure-function analysis of rotavirus NSP2 octamer by using a novel complementation system. J Virol 80:7984-7994.
10. Jayaram H, Taraporewala Z, Patton JT, Prasad BV (2002) Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold. Nature 417:311-315.
11. Jiang X, et al. (2006) Cryoelectron microscopy structures of rotavirus NSP2-NSP5 and NSP2-RNA complexes: Implications for genome replication. J Virol 80:10829-10835.
12. Schuck P, Taraporewala Z, McPhie P, Patton JT (2001) Rotavirus nonstructural protein NSP2 self-assembles into octamers that undergo ligand-induced conformational changes. J Biol Chem 276:9679-9687.
13. Taraporewala Z, Chen D, Patton JT (1999) Multimers formed by the rotavirus nonstructural protein NSP2 bind to RNA and have nucleoside triphosphatase activity. J Virol 73:9934-9943.
14. Taraporewala ZF, Patton JT (2001) Identification and characterization of the helixdestabilizing activity of rotavirus nonstructural protein NSP2. J Virol 75:4519-4527. (Pubitemid 32381494)
15. Vasquez-Del Carpio R, Gonzalez-Nilo FD, Riadi G, Taraporewala ZF, Patton JT (2006) Histidine triad-like motif of the rotavirus NSP2 octamer mediates both RTPase and NTPase activities. J Mol Biol 362:539-554.
16. Chen D, Gombold JL, Ramig RF (1990) Intracellular RNA synthesis directed by temperature-sensitive mutants of simian rotavirus SA11. Virology 178:143-151.
17. Miest T, Saenz D, Meehan A, Llano M, Poeschla EM (2009) Intensive RNAi with lentiviral vectors in mammalian cells. Methods 47:298-303.
18. Silvestri LS, Taraporewala ZF, Patton JT (2004) Rotavirus replication: Plus-sense templates for double-stranded RNA synthesis are made in viroplasms. J Virol 78:7763-7774.
19. Llano M, Gaznick N, Poeschla EM (2009) Rapid, controlled and intensive lentiviral vector-based RNAi. Methods Mol Biol 485:257-270.
20. Llano M, et al. (2006) An essential role for LEDGF/p75 in HIV integration. Science 314:461-464.
21. Ishii K, et al. (2002) Structural analysis of vaccinia virus DIs strain: Application as a new replication-deficient viral vector. Virology 302:433-444. (Pubitemid 35373871)
22. Taraporewala ZF, Schuck P, Ramig RF, Silvestri L, Patton JT (2002) Analysis of a temperature-sensitive mutant rotavirus indicates that NSP2 octamers are the functional form of the protein. J Virol 76:7082-7093. (Pubitemid 41246222)
23. Kalica AR, et al. (1978) Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247-255.
24. Ross J, Ostlund EN, Cao D, Tatsumi M, Hoshino Y (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-380.
25. Ramig RF (1983) Factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11. Virology 127:91-99. (Pubitemid 13058251)
26. Carpio RV, et al. (2004) Role of the histidine triad-like motif in nucleotide hydrolysis by the rotavirus RNA-packaging protein NSP2. J Biol Chem 279:10624-10633.
27. Patton JT, Wentz M, Xiaobo J, Ramig RF (1996) cis-acting signals that promote genome replication in rotavirus mRNA. J Virol 70:3961-3971. (Pubitemid 26000286)
28. Patton JT, Spencer E (2000) Genome replication and packaging of segmented doublestranded RNA viruses. Virology 277:217-225.
29. Heiman EM, et al. (2008) Group A human rotavirus genomics: Evidence that gene constellations are influenced by viral protein interactions. J Virol 82:11106-11116.
30. Gombold JL, Estes MK, Ramig RF (1985) Assignment of simian rotavirus SA11 temperature-sensitive mutant groups B and E to genome segments. Virology 143:309-320.
31. Gombold JL, Ramig RF (1987) Assignment of simian rotavirus SA11 temperature-sensitive mutant groups A, C, F, and G to genome segments. Virology 161:463-473.
32. Ramig RF (1983) Isolation and genetic characterization of temperature-sensitive mutants that define five additional recombination groups in simian rotavirus SA11. Virology 130:464-473.
33. Greenberg HB, et al. (1981) Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proc Natl Acad Sci USA 78:420-424.
34. Kalica AR, Flores J, Greenberg HB (1983) Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology 125:194-205. (Pubitemid 13149573)
35. Montero H, Arias CF, Lopez S (2006) Rotavirus nonstructural protein NSP3 is not required for viral protein synthesis. J Virol 80:9031-9038. (Pubitemid 44359977)
36. Arnold M, Patton JT, McDonald SM (2009) Culturing, storage, and quantification of rotaviruses. Curr Protoc Microbiol, Chap 15: Unit 15C.13.
37. Watanabe S, et al. (2004) Production of novel Ebola virus-like particles from cDNAs: An alternative to Ebola virus generation by reverse genetics. J Virol 78:999-1005.