Identification and characterization of the helix-destabilizing activity of rotavirus nonstructural protein NSP2

Journal of Virology

Volumn 75, Issue 10, 2001, Pages 4519-4527

  Taraporewala, Z F ^a   Patton, J T ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HELICASE; MAGNESIUM ION; NUCLEOSIDE TRIPHOSPHATASE; RNA; SINGLE STRANDED DNA BINDING PROTEIN; VIRUS PROTEIN;

ARTICLE; DNA REPLICATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN STABILITY; RNA REPLICATION; ROTAVIRUS; VIRUS ASSEMBLY;

ADENOSINE TRIPHOSPHATE; CALCIUM; CATIONS, DIVALENT; CYTIDINE TRIPHOSPHATE; DNA; GUANOSINE TRIPHOSPHATE; KINETICS; MAGNESIUM; NUCLEIC ACID HETERODUPLEXES; RNA; RNA-BINDING PROTEINS; ROTAVIRUS; URIDINE TRIPHOSPHATE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 0035034563     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.10.4519-4527.2001     Document Type: Article

References (44)

1. Recovery and characterization of a replicase complex in rotavirus-infected cells using a monoclonal antibody against NSP2
2. Herpes simplex virus type 1 ICP8: Helix-destabilizing properties
3. Characterization of virus inclusion bodies in bluetongue virus-infected cells
4. Intracellular RNA synthesis directed by temperature-sensitive mutants of simian rotavirus SA11
5. Template-dependent, in vitro replication of rotavirus RNA
6. Ribonucleic acid polymerase activity associated with purified calf rotavirus
7. Multimerization of the adenovirus DNA-binding protein is the driving force for ATP-independent DNA unwinding during strand displacement synthesis
8. The rotavirus genome
9. Characterization of rotavirus replication intermediates: A model for the assembly of single-shelled particles
10. Differential functional behavior of viral phi29, Nf and GA-1 SSB proteins
11. Amino terminus of reovirus nonstructural protein σNS is important for ssRNA binding and nucleoprotein complex formation
12. Reovirus protein sigma NS binds in multiple copies to single-stranded RNA and shares properties with single-stranded DNA binding proteins
13. Denaturation of T4 DNA by an in vitro processed gene 32 protein
14. In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for their single-stranded RNA
15. Virus-encoded RNA helicases
16. Efficacy of a quadrivalent rhesus rotavirus-based human rotavirus vaccine aimed at preventing severe rotavirus diarrhea in infants and young children
17. The rotavirus RNA binding protein NS35 (NSP2) forms 10S multimers and interacts with the viral RNA polymerase
18. The rotavirus nonstructural protein, NS35, is a nonspecific RNA-binding protein
19. Phosphorylation and other properties of proteins binding single-stranded DNA (SSB-proteins) from chromatin and extrachromatin fractions of Ehrlich ascites carcinoma
20. Three-dimensional structural analysis of recombinant rotavirus-like particles with intact and amino-terminal deleted VP2: Implications for the architecture of the VP2 capsid layer
21. Three-dimensional visualization of mRNA release from actively transcribing rotavirus particles
22. Reovirus μ2 protein determines strain-specific differences in the rate of viral inclusion formation in L929 cells
23. Helix-destabilizing properties of the baculovirus single-stranded DNA-binding protein (LEF-3)
24. Precise packaging of the three genomic segments of the double-stranded-RNA bacteriophage phi6
25. On the role of magnesium ions on RNA stability
26. Adenovirus DNA binding protein: Helix-destabilizing activity
27. Rotavirus VP1 alone specifically binds to the 3′ end of viral mRNA but the interaction is not sufficient to initiate minus-strand synthesis
28. RNA-binding and capping activities of proteins in rotavirus open cores
29. Cis-acting signals that promote genome replication in rotavirus mRNA
30. Structure and protein composition of the rotavirus replicase particle
31. Rotavirus RNA replication: Single-stranded RNA extends from the replicase particle
32. Ultrastructural localization of rotavirus antigens using colloidal gold
33. Three-dimensional structure of rotavirus
34. Characterization of temperature sensitive mutants of simian rotavirus SA11: Protein synthesis and morphogenesis
35. Characterization of the single-stranded DNA binding protein encoded by the vaccinia virus 13 gene
36. Rotavirus nonstructural protein NSP2 self-assembles into octamers that undergo ligand-induced conformational changes
37. Knotting of a DNA chain during ring closure
38. Helix-destabilizing activity of phi 29 single-stranded DNA binding protein: Effect on the elongation rate during strand displacement DNA replication
39. Multimers formed by the rotavirus nonstructural protein NSP2 bind to RNA and have nucleoside triphosphatase activity
40. Synthesis of bluetongue virus-encoded phosphoprotein and formation of inclusion bodies by recombinant baculovirus in insect cells: It binds the single-stranded RNA species
41. Overexpression, purification and helix-destabilizing properties of Epstein-Barr virus ssDNA-binding protein
42. The multimeric nonstructural NS2 proteins of bluetongue virus, African horsesickness virus and epizootic hemorrhagic disease virus differ in their singlestranded RNA-binding ability
43. Winding of DNA helix by divalent cations