Multimers formed by the rotavirus nonstructural protein NSP2 bind to RNA and have nucleoside triphosphatase activity

Journal of Virology

Volumn 73, Issue 12, 1999, Pages 9934-9943

  Taraporewala, Zenobia ^a   Chen, Dayue ^a   Patton, John T ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL INCLUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; ROTAVIRUS; VIRUS ASSEMBLY; VIRUS REPLICATION;

EID: 0032759060     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.12.9934-9943.1999     Document Type: Article

References (42)

1. Afrikanova, I., E. Fabretti, M. C. Miozzo, and O. R. Burrone. 1998. Rotavirus NSP5 phosphorylation is up-regulated by interaction with NSP2. J. Gen. Virol. 79:2679-2686.
2. Aponte, C., D. Poncet, and J. Cohen. 1996. Recovery and characterization of a replicase complex in rotavirus-infected cells using a monoclonal antibody against NSP2. J. Virol. 70:985-991.
3. Bican, P., J. Cohen, A. Charpilienne, and R. Scherrer. 1982. Purification and characterization of bovine rotavirus cores. J. Virol. 43:1113-1117.
4. Blackhall, J., A. Fuentes, K. Hansen, and G. Magnusson. 1997. Serine protein kinase activity associated with rotavirus phosphoprotein NSP5. J. Virol. 71:138-144.
5. Chen, D. J. L. Gombold, and R. F. Ramig. 1990. Intracellular RNA synthesis directed by temperature-sensitive mutants of simian rotavirus SA11. Virology 178:143-151.
6. Chen, D. Y., C. Y. Zeng, M. J. Wentz, M. Gorziglia, M. K. Estes, and R. F. Ramig. 1994. Template-dependent, in vitro replication of rotavirus RNA. J. Virol. 68:7030-7039.
7. Christensen, M. S. 1989. Human viral gastroenteritis. Clin. Microbiol. Rev. 2:51-89.
8. Cohen, J. 1977. Ribonucleic acid polymerase activity associated with purified calf rotavirus. J. Gen. Virol. 36:395-402.
9. Fabbretti, E., I. Afrikanova, F. Vascotto, and O. R. Burrone. 1999. Two nonstructural rotavirus proteins, NSP2 and NSP5, form viroplasm-like structures in vivo. J. Gen. Virol. 80:333-339.
10. Fuerst, R. T., G. E. Niles, W. F. Studier, and B. Moss. 1986. Eucaryotic transient-expression system based on recombinant vaccinia virus that synthesized bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
11. Galiegos, C. O., and J. T. Patton. 1989. Characterization of rotavirus replication intermediates: a model for the assembly of single-shelled particles. Virology 172:616-627.
12. Gillian, A. L., and M. L. Nibert. 1998. Amino terminus of reovirus nonstructural protein σNS is important for ssRNA binding and nucleoprotein complex formation. Virology 240:1-11.
13. Gonzales, S. A., and O. Burrone. 1991. Rotavirus NS26 is modified by addition of a single O-linked residue of N-acetylglucosamine. Virology 182: 8-16.
14. Helmberger-Jones, M., and J. T. Patton. 1986. Characterization of subviral particles in cells infected with simian rotavirus SA11. Virology 155:655-665.
15. Huismans, H., and W. K. Joklik. 1972. Reovirus-coded polypeptides in infected cells: Isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology 70: 411-424.
16. Kattoura, M. D., X. Chen, and J. T. Patton. 1994. The rotavirus RNA-binding protein NS35 (NSP2) forms 10S multimers and interacts with the viral RNA polymerase. Virology 202:803-813.
17. Kattoura, M. D., L. L. Clapp, and J. T. Patton. 1992. The rotavirus non-structural protein, NS35, is a nonspecific RNA-binding protein. Virology 191:698-708.
18. Laemmli, U. K. 1970. Cleavage of the structural proteins during the assembly of the head bacteriophage T4. Nature 227:680-685.
19. Lawton, J. A., C. Q.-Y. Zeng, S. K. Mukherjee, J. Cohen, M. K. Estes, and B. V. V. Prasad. 1997. Three-dimensional structural analysis of recombinant rotavirus-like particles with intact and amino-terminal deleted VP2: implications for the architecture of the VP2 capsid layer. J. Virol. 71:7353-7360.
20. Lui, M., N. M. Mattion, and M. K. Estes. 1992. Rotavirus VP3 expressed in insect cells possesses guanylyltransferase activity. Virology 188:77-84.
21. Mansell, E. A., and J. T. Patton. 1990. Rotavirus RNA replication: VP2, but not VP6, is necessary for viral replicase activity. J. Virol. 64:4988-4996.
22. Midthun, K., H. B. Greenberg, Y. Hoshino, A. Z. Kapikian, R. G. Wyatt, and R. M. Chanock. 1985. Reassortant rotaviruses as potential live rotavirus vaccine candidates. J. Virol. 53:949-954.
23. Paatero, A. O., J. E. Syvaoja, and D. H. Bamford. 1995. Double-stranded RNA bacteriophage φ6 protein P4 is an unspecific nucleoside triphosphatase activated by calcium ions. J. Virol. 69:6729-6734.
24. Patton, J. T. 1986. Synthesis of simian rotavirus SA11 double stranded RNA in a cell-free system. Virus Res. 6:217-223.
25. Patton, J. T. 1996. Rotavirus VP1 alone specifically binds to the 3′ end of viral mRNA but the interaction is not sufficient to initiate minus-strand synthesis. J. Virol. 70:7940-7947.
26. Patton, J. T., and D. Chen. 1999. RNA-binding and capping activities of proteins in rotavirus open cores. J. Virol. 73:1382-1391.
27. Patton, J. T., and C. O. Gallegos. 1988. Structure and protein composition of the rotavirus replicase particle. Virology 166:358-365.
28. Patton, J. T., and C. O. Gallegos. 1990. Rotavirus RNA replication: single-stranded RNA extends from the replicase particle. J. Gen. Virol. 71:1087-1094.
29. Patton, J. T., M. T. Jones, A. N. Kalbach, Y. W. He, and J. Xiaobo, 1997. Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome. J. Virol. 71:9618-9626.
30. Patton, J. T., L. Salter-Cid, A. Kalbach, A. E. Mansell, and M. D. Kattoura. 1993. Nucleotide and amino acid sequence analysis of the rotavirus non-structural RNA binding protein NS35. Virology 192:438-446.
31. Patton, J. T., M. Wentz, J. Xiaobo, and R. F. Ramig. 1996. cis-acting signals that promote genome replication in rotavirus mRNAs. J. Virol. 70:3961-3971.
32. Petrie, B. L., H. B. Greenberg, D. Y. Graham, and M. K. Estes. 1984. Ultrastructural localization of rotavirus antigens using colloidal gold. Virus Res. 1:133-152.
33. Pizarro, J. L., A. M. Sandino, J. M. Pizarro, J. Fernandez, and E. Spencer. 1991. Characterization of rotavirus guanylyltransferase activity associated with polypeptide VP3. J. Gen. Virol. 72:325-332.
34. Poncet, D., P. Lindenbaum, R. L. Haridon, and J. Cohen. 1997. In vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms. J. Virol. 71:34-41.
35. Prasad, B. V. V., G. J. Wang, J. P. M. Clerx, and W. Chiu. 1988. Three-dimensional structure of rotavirus. J. Mol. Biol. 199:269-275.
36. Ramig, R. F. 1982. Isolation and genetic characterization of temperature-sensitive mutants of simian rotavirus SA11. Virology 120:93-105.
37. Ramig, R. F., and B. L. Petrie. 1984. Characterization of temperature-sensitive mutants of simian rotavirus SA11: protein synthesis and morphogenesis. J. Virol. 49:665-673.
38. Thomas, C. P., T. F. Booth, and P. Roy. 1990. Synthesis of bluetongue virus encoded phosphoprotein and formation of inclusion bodies by recombinant baculovirus in insect cells: it binds the single stranded RNA species. J. Gen. Virol. 71:2073-2083.
39. Uitenweerde, J. M., J. Theron, M. A. Stoltz, and H. Huismans. 1995. The multimeric nonstructural proteins of bluetongue virus, African horsesickness virus, and epizootic haemorrhagic disease virus differ in their single-stranded RNA binding ability. Virology 209:624-632.
40. Welch, S. K., S. E. Crawford, and M. K. Estes. 1989. Rotavirus SA11 genome segment 11 protein is a nonstructural phosphoprotein. J. Virol. 63:3974-3982.
41. Yeager, M., K. A. Dryden, N. H. Olsen, H. B. Greenberg, and T. S. Baker. 1990. Three-dimensional structure of rhesus rotavirus by cryoelectron microscopy and image reconstruction. J. Cell Biol. 110:2133-2144.
42. Zeng, C. Q.-Y., M. J. Wentz, M. K. Estes, and R. F. Ramig. 1996. Characterization and replicase activity of double-layered and single-layered rotavirus-like particles expressed from baculovirus recombinants. J. Virol. 70: 2736-2742.