Metabolic engineering of seeds can achieve levels of ω-7 fatty acids comparable with the highest levels found in natural plant sources

Plant Physiology

Volumn 154, Issue 4, 2010, Pages 1897-1904

  Nguyen, Huu Tam ^a,c   Mishra, Girish ^a,d   Whittle, Edward ^a   Bevan, Scott A ^b   Merlo, Ann Owens ^b   Walsh, Terence A ^b   Shanklin, John ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b DOW AGROSCIENCES LLC   (United States)

^c UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^d UNIVERSITY OF DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA; DOXANTHA; MACFADYENA UNGUIS-CATI;

EID: 78649789497     PISSN: 00320889     EISSN: 15322548     Source Type: Journal    
DOI: 10.1104/pp.110.165340     Document Type: Article

References (34)

1. Anonymous (2007) Process Evaluation/Research Planning Report, On-Purpose Octene-1. Nexant ChemSystems, White Plains, NY
2. Bondaruk M, Johnson S, Degafu A, Boora P, Bilodeau P, Morris J, Wiehler W, Foroud N, Weselake R, Shah S (2007) Expression of a cDNA encoding palmitoyl-acyl carrier protein desaturase from cat's claw (Doxantha unguis-cati L.) in Arabidopsis thaliana and Brassica napus leads to accumulation of unusual unsaturated fatty acids and increased stearic acid content in the seed oil. Plant Breed 126: 186-194
3. Burgal J, Shockey J, Lu C, Dyer J, Larson T, Graham I, Browse J (2008) Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnol J 6: 819-831
4. Butte W, Eilers J, Hirsch K (1982) Trialkysulfonium-hydroxides and trialkylselonium-hydroxides for the pyrolytic alkylation of acidic compounds. Anal Lett 15: 841-850
5. Cahoon EB, Coughlan SJ, Shanklin J (1997) Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed. Plant Mol Biol 33: 1105-1110
6. Cahoon EB, Shah S, Shanklin J, Browse J (1998) A determinant of substrate specificity predicted from the acyl-acyl carrier protein de-saturase of developing cat's claw seed. Plant Physiol 117: 593-598
7. Carlsson AS (2009) Plant oils as feedstock alternatives to petroleum - A short survey of potential oil crop platforms. Biochimie 91: 665-670
8. Carlsson AS, LaBrie ST, Kinney AJ, von Wettstein-Knowles P, Browse J (2002) A KAS2 cDNA complements the phenotypes of the Arabidopsis fab1 mutant that differs in a single residue bordering the substrate binding pocket. Plant J 29: 761-770
9. Chisholm MJ, Hopkins CY (1965) Fatty acids of Doxantha seed oil. J Am Oil Chem Soc 42: 49-50
10. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agro-bacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743
11. Dyer JM, Stymne S, Green AG, Carlsson AS (2008) High-value oils from plants. Plant J 54: 640-655
12. Hopkins CY, Chisholm MJ (1961) Development of oil in the seed of Asclepias syriaca L. Can J Biochem Physiol 39: 829-835
13. James DW, Dooner HK (1990) Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition. Theor Appl Genet 80: 241-245
14. Jones A, Davies HM, Voelker TA (1995) Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7: 359-371
15. Knutzon DS, Hayes TR, Wyrick A, Xiong H, Davies M, Voelker TA (1999) Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiol 120: 739-746
16. Lee M, Lenman M, Banaś A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, et al (1998) Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation. Science 280: 915-918
17. Meier MAR (2009) Metathesis with oleochemicals: new approaches for the utilization of plant oils as renewable resources in plant science. Macro-mol Chem Phys 210: 1073-1079
18. Millar AA, Smith MA, Kunst L (2000) All fatty acids are not equal: discrimination in plant membrane lipids. Trends Plant Sci 5: 95-101
19. Napier JA (2007) The production of unusual fatty acids in transgenic plants. Annu Rev Plant Biol 58: 295-319
20. Nguyen HT, Shanklin J (2009) Altering Arabidopsis oilseed composition by a combined antisense-hairpin RNAi gene suppression approach. J Am Oil Chem Soc 86: 41-49
21. Pidkowich MS, Nguyen HT, Heilmann I, Ischebeck T, Shanklin J (2007) Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. Proc Natl Acad Sci USA 104: 4742-4747
22. Rybak A, Fokou PA, Meier MAR (2008) Metathesis as a versatile tool in oleochemsitry. Eur J Lipid Sci Technol 110: 797-804
23. Salas JJ, Ohlrogge JB (2002) Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases. Arch Biochem Biophys 403: 25-34
24. Schultz DJ, Suh MC, Ohlrogge JB (2000) Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin. Plant Physiol 124: 681-692
25. Shanklin J, Cahoon EB (1998) Desaturation and related modifications of fatty acids. Annu Rev Plant Physiol Plant Mol Biol 49: 611-641
26. Shockey JM, Fulda MS, Browse J (2003) Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases. Plant Physiol 132: 1065-1076
27. Slightom JL, Sun SM, Hall TC (1983) Complete nucleotide sequence of a French bean storage protein gene: Phaseolin. Proc Natl Acad Sci USA 80: 1897-1901
28. Stuitje AR, Verbree EC, van der Linden KH, Mietkiewska EM, Nap JP, Kneppers TJ (2003) Seed-expressed fluorescent proteins as versatile tools for easy (co)transformation and high-throughput functional ge-nomics in Arabidopsis. Plant Biotechnol J 1: 301-309
29. Stukey JE, McDonough VM, Martin CE (1990) The OLE1 gene of Saccha-romyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene. J Biol Chem 265: 20144-20149
30. Suh MC, Schultz DJ, Ohlrogge JB (1999) Isoforms of acyl carrier protein involved in seed-specific fatty acid synthesis. Plant J 17: 679-688
31. Suh MC, Schultz DJ, Ohlrogge JB (2002) What limits production of unusual monoenoic fatty acids in transgenic plants? Planta 215: 584-595
32. van der Geest AH, Hall TC (1997) The beta-phaseolin 5# matrix attachment region acts as an enhancer facilitator. Plant Mol Biol 33: 553-557
33. Whittle E, Shanklin J (2001) Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation muta-genesis and logical redesign of the castor delta 9-18:0-ACP desaturase. J Biol Chem 276: 21500-21505
34. Yamamoto K, Shibahara A, Nakayama T, Kajimoto G (1991) Determination of double-bond positions in methylene-interrupted dienoic fatty acids by GC-MS as their dimethyl disulfide adducts. Chem Phys Lipids 60: 39-50