Antiviral and neuroprotective role of octaguanidinium dendrimer-conjugated Morpholino oligomers in Japanese encephalitis

PLoS Neglected Tropical Diseases

Volumn 4, Issue 11, 2010, Pages

  Nazmi, Arshed ^a   Dutta, Kallol ^a   Basu, Anirban ^a  

^a NATIONAL BRAIN RESEARCH CENTRE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 6; MONOCYTE CHEMOTACTIC PROTEIN 1; MORPHOLINE DERIVATIVE; OCTAGUANIDINIUM DENDRIMER CONJUGATED MORPHOLINO OLIGOMER; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; VIRUS PROTEIN; ANTISENSE OLIGODEOXYNUCLEOTIDE; ANTIVIRUS AGENT; CYTOKINE; DENDRIMER; NEUROPROTECTIVE AGENT; OLIGONUCLEOTIDE;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIVIRAL ACTIVITY; ARTICLE; BRAIN HOMOGENATE; BRAIN LEVEL; CELL ACTIVATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DRUG EFFICACY; DRUG TARGETING; FEMALE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; JAPANESE ENCEPHALITIS; JAPANESE ENCEPHALITIS VIRUS; MALE; MICROGLIA; MOUSE; NEUROBLASTOMA CELL; NEUROPROTECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PROTEIN EXPRESSION; SURVIVAL RATE; VERO CELL; VIRUS GENOME; VIRUS INHIBITION; VIRUS LOAD; VIRUS PARTICLE; ANIMAL; BAGG ALBINO MOUSE; BRAIN; CERCOPITHECUS; DRUG EFFECT; GENETICS; HUMAN; IMMUNOLOGY; PHYSIOLOGY; VIROLOGY; VIRUS REPLICATION;

ANIMALS; ANTIVIRAL AGENTS; BRAIN; CERCOPITHECUS AETHIOPS; CYTOKINES; DENDRIMERS; ENCEPHALITIS VIRUS, JAPANESE; ENCEPHALITIS, JAPANESE; HUMANS; MICE; MICE, INBRED BALB C; MORPHOLINES; NEUROPROTECTIVE AGENTS; OLIGODEOXYRIBONUCLEOTIDES, ANTISENSE; OLIGONUCLEOTIDES; VERO CELLS; VIRAL LOAD; VIRUS REPLICATION;

EID: 78649770754     PISSN: None     EISSN: None     Source Type: Journal    
DOI: 10.1371/journal.pntd.0000892     Document Type: Article

References (41)

1. Ghosh D, Basu A (2008) Present perspectives on flaviviral chemotherapy. Drug Discov Today 13: 619-624.
2. Solomon T, Ni H, Beasley DW, Ekkelenkamp M, Cardosa MJ, et al. (2003) Origin and evolution of Japanese encephalitis virus in southeast Asia. J Virol 77: 3091-3098.
3. Ghosh D, Basu A (2009) Japanese encephalitis-a pathological and clinical perspective. PLoS Negl Trop Dis 3: e437.
4. Saxena V, Dhole TN (2008) Preventive strategies for frequent outbreaks of Japanese encephalitis in Northern India. J Biosci 33: 505-514.
5. Vrati S, Giri RK, Razdan A, Malik P (1999) Complete nucleotide sequence of an Indian strain of Japanese encephalitis virus: sequence comparison with other strains and phylogenetic analysis. Am J Trop Med Hyg 61: 677-680.
6. Sumiyoshi H, Mori C, Fuke I, Morita K, Kuhara S, et al. (1987) Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 161: 497-510.
7. Chambers TJ, Hahn CS, Galler R, Rice CM (1990) Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44: 649-688.
8. Khromykh AA, Meka H, Guyatt KJ, Westaway EG (2001) Essential role of cyclization sequences in flavivirus RNA replication. J Virol 75: 6719-6728.
9. Villordo SM, Gamarnik AV (2009) Genome cyclization as strategy for flavivirus RNA replication. Virus Res 139: 230-239.
10. Kumar P, Lee SK, Shankar P, Manjunath N (2006) A single siRNA suppresses fatal encephalitis induced by two different flaviviruses. PLoS Med 3: e96.
11. Liu X, Cao S, Zhou R, Xu G, Xiao S, et al. (2006) Inhibition of Japanese encephalitis virus NS1 protein expression in cell by small interfering RNAs. Virus Genes 33: 69-75.
12. Qi WB, Hua RH, Yan LP, Tong GZ, Zhang GH, et al. (2008) Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Virus Res 132: 145-151.
13. Appaiahgari MB, Vrati S (2007) DNAzyme-mediated inhibition of Japanese encephalitis virus replication in mouse brain. Mol Ther 15: 1593-1599.
14. Deas TS, Bennett CJ, Jones SA, Tilgner M, Ren P, et al. (2007) In vitro resistance selection and in vivo efficacy of morpholino oligomers against West Nile virus. Antimicrob Agents Chemother 51: 2470-2482.
15. Anantpadma M, Stein DA, Vrati S (2010) Inhibition of Japanese encephalitis virus replication in cultured cells and mice by a peptide-conjugated morpholino oligomer. J Antimicrob Chemother 65: 953-961.
16. Summerton J, Weller D (1997) Morpholino antisense oligomers: design, preparation, and properties. Antisense Nucleic Acid Drug Dev 7: 187-195.
17. Moulton HM, Nelson MH, Hatlevig SA, Reddy MT, Iversen PL (2004) Cellular uptake of antisense morpholino oligomers conjugated to arginine-rich peptides. Bioconjug Chem 15: 290-299.
18. Morcos PA, Li Y, Jiang S (2008) Vivo-Morpholinos: a non-peptide transporter delivers Morpholinos into a wide array of mouse tissues. Biotechniques 45: 613-614, 616, 618 passim.
19. Das S, Ghosh D, Basu A (2009) Japanese encephalitis virus induce immunocompetency in neural stem/progenitor cells. PLoS One 4: e8134.
20. Mishra MK, Basu A (2008) Minocycline neuroprotects, reduces microglial activation, inhibits caspase 3 induction, and viral replication following Japanese encephalitis. J Neurochem 105: 1582-1595.
21. Dutta K, Ghosh D, Nazmi A, Kumawat KL, Basu A (2010) A common carcinogen benzo[a]pyrene causes neuronal death in mouse via microglial activation. PLoS One 5: e9984.
22. Dutta K, Ghosh D, Basu A (2009) Curcumin protects neuronal cells from Japanese encephalitis virus-mediated cell death and also inhibits infective viral particle formation by dysregulation of ubiquitin-proteasome system. J Neuroimmune Pharmacol 4: 328-337.
23. Mishra MK, Dutta K, Saheb SK, Basu A (2009) Understanding the molecular mechanism of blood-brain barrier damage in an experimental model of Japanese encephalitis: correlation with minocycline administration as a therapeutic agent. Neurochem Int 55: 717-723.
24. Ghoshal A, Das S, Ghosh S, Mishra MK, Sharma V, et al. (2007) Proinflammatory mediators released by activated microglia induces neuronal death in Japanese encephalitis. Glia 55: 483-496.
25. Saxena SK, Mathur A, Srivastava RC (2001) Induction of nitric oxide synthase during Japanese encephalitis virus infection: evidence of protective role. Arch Biochem Biophys 391: 1-7.
26. Murakami M, Ota T, Nukuzuma S, Takegami T (2005) Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo. Microbiol Immunol 49: 1047-1056.
27. Kinney RM, Huang CY, Rose BC, Kroeker AD, Dreher TW, et al. (2005) Inhibition of dengue virus serotypes 1 to 4 in vero cell cultures with morpholino oligomers. J Virol 79: 5116-5128.
28. Wender PA, Mitchell DJ, Pattabiraman K, Pelkey ET, Steinman L, et al. (2000) The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters. Proc Natl Acad Sci U S A 97: 13003-13008.
29. Amantana A, Moulton HM, Cate ML, Reddy MT, Whitehead T, et al. (2007) Pharmacokinetics, biodistribution, stability and toxicity of a cell-penetrating peptide-morpholino oligomer conjugate. Bioconjug Chem 18: 1325-1331.
30. Moulton HM, Fletcher S, Neuman BW, McClorey G, Stein DA, et al. (2007) Cell-penetrating peptide-morpholino conjugates alter pre-mRNA splicing of DMD (Duchenne muscular dystrophy) and inhibit murine coronavirus replication in vivo. Biochem Soc Trans 35: 826-828.
31. Lebleu B, Moulton HM, Abes R, Ivanova GD, Abes S, et al. (2008) Cell penetrating peptide conjugates of steric block oligonucleotides. Adv Drug Deliv Rev 60: 517-529.
32. Li YF, Morcos PA (2008) Design and synthesis of dendritic molecular transporter that achieves efficient in vivo delivery of morpholino antisense oligo. Bioconjug Chem 19: 1464-1470.
33. Moulton JD, Jiang S (2009) Gene knockdowns in adult animals: PPMOs and vivo-morpholinos. Molecules 14: 1304-1323.
34. Issur M, Geiss BJ, Bougie I, Picard-Jean F, Despins S, et al. (2009) The flavivirus NS5 protein is a true RNA guanylyltransferase that catalyzes a two-step reaction to form the RNA cap structure. Rna 15: 2340-2350.
35. Solomon T (2003) Recent advances in Japanese encephalitis. J Neurovirol 9: 274-283.
36. Halliwell B (2001) Role of free radicals in the neurodegenerative diseases: therapeutic implications for antioxidant treatment. Drugs Aging 18: 685-716.
37. Lin YL, Huang YL, Ma SH, Yeh CT, Chiou SY, et al. (1997) Inhibition of Japanese encephalitis virus infection by nitric oxide: antiviral effect of nitric oxide on RNA virus replication. J Virol 71: 5227-5235.
38. Swarup V, Ghosh J, Ghosh S, Saxena A, Basu A (2007) Antiviral and anti-inflammatory effects of rosmarinic acid in an experimental murine model of Japanese encephalitis. Antimicrob Agents Chemother 51: 3367-3370.
39. Su HL, Liao CL, Lin YL (2002) Japanese encephalitis virus infection initiates endoplasmic reticulum stress and an unfolded protein response. J Virol 76: 4162-4171.
40. Munoz L, Ranaivo HR, Roy SM, Hu W, Craft JM, et al. (2007) A novel p38 alpha MAPK inhibitor suppresses brain proinflammatory cytokine up-regulation and attenuates synaptic dysfunction and behavioral deficits in an Alzheimer's disease mouse model. J Neuroinflammation 4: 21.
41. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31: 3406-3415.