The crystal structure of OprG from Pseudomonas aeruginosa, a potential channel for transport of hydrophobic molecules across the outer membrane

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Touw, Debra S ^a   Patel, Dimki R ^a   van den Berg, Bert ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN; PROTEIN OMPW; PROTEIN OPRG; UNCLASSIFIED DRUG; ION CHANNEL; OPRG PROTEIN, PSEUDOMONAS AERUGINOSA; OUTER MEMBRANE PROTEIN;

ARTICLE; BACTERIAL OUTER MEMBRANE; CRYSTAL STRUCTURE; CYTOPLASM; DIFFUSION; HYDROPHOBICITY; MEMBRANE TRANSPORT; NONHUMAN; PROTEIN FAMILY; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; PSEUDOMONAS AERUGINOSA; SEQUENCE ALIGNMENT; X RAY CRYSTALLOGRAPHY; BIOLOGICAL MODEL; CELL MEMBRANE; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; TRANSPORT AT THE CELLULAR LEVEL;

NEGIBACTERIA; PSEUDOMONAS AERUGINOSA;

BACTERIAL OUTER MEMBRANE PROTEINS; BIOLOGICAL TRANSPORT; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; ION CHANNELS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; PERIPLASM; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY;

EID: 78649749532     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015016     Document Type: Article

References (32)

1. Mesaros N, Nordmann P, Plésiat P, Roussel-Delvallez M, Eldere JV, et al. (2007) Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clin Microbiol Inf 13: 560-578.
2. Hancock REW, Brinkman FSL (2002) Function of Pseudomonas Porin in Uptake and Efflux. Ann Rev Microbiol 56: 17-38.
3. Nikaido H (2003) Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiol Mol Biol Rev 67: 593-656.
4. Pawelek PD, Croteau N, Ng-Thow-Hing C, Khursigara CM, Moiseeva N, et al. (2006) Structure of TonB in Complex with FhuA, E. coli Outer Membrane Receptor. Science 312: 1399-1402.
5. Buchanan SK, Smith BS, Venkatramani L, Xia D, Esser L, et al. (1999) Crystal structure of the outer membrane active transporter FepA from Escherichia coli. Nat Struct Mol Biol 6: 56-63.
6. Cowan SW, Schirmer T, Rummel G, Steiert M, Ghosh R, et al. (1992) Crystal structures explain functional properties of two E. coli porins. Nature 358: 727-733.
7. van den Berg B (2005) The FadL family: unusual transporters for unusual substrates. Curr Opin Struct Biol 15: 401-407.
8. Hearn EM, Patel DR, Lepore BW, Indic M, van den Berg B (2009) Transmembrane passage of hydrophobic compounds through a protein channel wall. Nature 458: 367-370.
9. Das M, Chopra AK, Cantu JM, Peterson JW (1998) Antisera to selected outer membrane proteins of Vibrio cholerae protect against challenge with homologous and heterologous strains of V. cholerae. FEMS Immunology & Medical Microbiology 22: 303-308.
10. Jalajakumari MB, Manning PA (1990) Nucleotide sequence of the gene, ompW, encoding a 22 kDa immunogenic outer membrane protein of Vibrio cholerae. Nucl Acids Res 18: 2180-.
11. Söderblom T, Oxhamre C, Wai SN, Uhlén P, Aperia A, et al. (2005) Effects of the Escherichia coli toxin cytolysin A on mucosal immunostimulation via epithelial Ca2+ signalling and Toll-like receptor 4. Cell Microbiol 7: 779-788.
12. Arnold T, Zeth K, Linke D (2009) Structure and Function of Colicin S4, a Colicin with a Duplicated Receptor-binding Domain. J Biol Chemistry 284: 6403-6413.
13. Wu L, Lin X, Wang F, Ye D, Xiao X, et al. (2006) OmpW and OmpV Are Required for NaCl Regulation in Photobacterium damsela. J Prot Res 5: 2250-2257.
14. van Beilen JB, Eggink G, Enequist H, Bors R, Wiltholt B (1992) DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans. Mol Microbiol 6: 3121-3136.
15. Eaton RW (1994) Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid. J Bacteriol 176: 7757-7762.
16. Denome SA, Stanley DC, Olson ES, Young KD (1993) Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway. J Bacteriol 175: 6890-6901.
17. Hong H, Patel DR, Tamm LK, van den Berg B (2006) The Outer Membrane Protein OmpW Forms an Eight-stranded b-Barrel with a Hydrophobic Channel. J Biol Chem 281: 7568-7577.
18. Neher TM, Lueking DR (2009) Pseudomonas fluorescens ompW: plasmid localization and requirement for napthalene uptake. Can J Microbiol 55: 553-563.
19. Gensberg K, Smith AW, Brinkman FSL, Hancock REW (1999) Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa. J Antimicrob Chemother 43: 607-608.
20. Peng X, Xu C, Ren H, Lin X, Wu L, et al. (2005) Proteomic Analysis of the Sarcosine-Insoluble Outer Membrane Fraction of Pseudomonas aeruginosa Responding to Ampicilin, Kanamycin, and Tetracycline Resistance. J Prot Res 4: 2257-2265.
21. McPhee JB, Tamber S, Bains M, Maier E, Gellatly S, et al. (2009) The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel. FEMS Microbiol Lett 296: 241-247.
22. Walzer G, Rosenberg E, Ron EZ (2009) Identification of outer membrane proteins with emulsifying activity by prediction of [beta]-barrel regions. J Microbact Methods 76: 52-57.
23. Pautsch A, Schulz GE (1998) Structure of the outer membrane protein A transmembrane domain. Nat Struct Mol Biol 5: 1013-1017.
24. Hong H, Szabo G, Tamm LK (2006) Electrostatic couplings in OmpA ionchannel gating suggest a mechanism for pore opening. Nat Chem Biol 2: 627-635.
25. Buehler LK, Kusumoto S, Zhang H, Rosenbusch JP (1991) Plasticity of Escherichia coli porin channels. Dependence of their conductance on strain and lipid environment. J Biol Chem 266: 24446-24450.
26. van den Berg B, Black PN, Clemons WM, Jr., Rapoport TA (2004) Crystal Structure of the Long-Chain Fatty Acid Transporter FadL. Science 304: 1506-1509.
27. Guzman L, Belin D, Carson M, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177: 4121-4130.
28. Otwinowski Z, Minor W (1997) Processing of X-ray Diffraction Data Collected in Oscillation Mode. In: Carter CW, Jr., Sweet RM, eds. Methods in Enzymology. New York: Academic Press. pp 307-326.
29. Zwart PH, Afonine PV, Grosse-Kunstleve RW, Hung L-W, Ioerger TR, et al. (2008) Automated Structure Solution with the PHENIX Suite. In: Kobe B, Guss M, Huber T, eds. Methods in Molecular Biology: Humana Press. pp 419-435.
30. Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallographica Section D 60: 2126-2132.
31. Davis IW, Leaver-Fay A, Chen VB, Block JN, Kapral GJ, et al. (2007) MolProbity: all-atom contacts and structure validation for proteins and nucleic acids. Nucl Acids Res 35: W375-W383.
32. DeLano WL (2002) PyMOL Molecular Graphics System. San Carlos, CA, USA: Delano Scientific.