Structure of TonB in complex with FhuA, E. coli outer membrane receptor

Science

Volumn 312, Issue 5778, 2006, Pages 1399-1402

  Pawelek, Peter D ^a   Croteau, Nathalie ^a   Ng Thow Hing, Christopher ^a   Khursigara, Cezar M ^a   Moiseeva, Natalia ^b   Allaire, Marc ^b   Coulton, James W ^a  

^a MCGILL UNIVERSITY   (Canada)

^b BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOLOGICAL MEMBRANES; CRYSTAL STRUCTURE; ESCHERICHIA COLI;

AMINO-TERMINAL RESIDUES; MEMBRANE RECEPTORS; SIDEROPHORE TRANSPORT;

PROTEINS;

FERRIC HYDROXAMATE UPTAKE PROTEIN COMPONENT A; FERRICHROME; MEMBRANE PROTEIN; PROTEIN TONB; SIDEROPHORE; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; CYTOPLASM; PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; CYTOPLASM; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE;

BACTERIAL OUTER MEMBRANE PROTEINS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERRIC COMPOUNDS; MEMBRANE PROTEINS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SURFACE PLASMON RESONANCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 33744791187     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1128057     Document Type: Article

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19. We thank A. Berghuis (McGill University, Montreal, Quebec) and M. Cygler (Biotechnology Research Institute, National Research Council, Montreal, Quebec) for access to home source x-rays and for helpful discussions. F. Rotella and S. L. Ginell at the Advanced Photon Source, beamline 19-ID, assisted with data collection. Use of the Argonne National Laboratory Structural Biology Center beamlines at the Advanced Photon Source was supported by the U.S. Department of Energy, Office of Biological and Environmental Research, under Contract No. W-31-109-ENG-38. We acknowledge the suggestions of K. Diederichs (Universität Konstanz, Germany) concerning site-directed mutagenesis to improve crystal contacts of FhuA. Critical reviews of this manuscript were provided by A. Berghuis, H. Le Moual, and B. Nagar, all at McGill University; J. A. Kashul edited the document. This work was supported by operating grant MOP-14133 (to J.W.C.) from the Canadian Institutes of Health Research. Infrastructure from Canada Foundation for Innovation was awarded to the Montreal Integrated Genomics Group for Research on Infectious Pathogens. Coordinates and structure factors have been deposited in the Protein Data Bank (PDB) with the accession code 2GRX.