Analysis of mice lacking DNasei hypersensitive sites at the 5' end of the IgH locus

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Perlot, Thomas ^a,c   Pawlitzky, Inka ^b,d   Manis, John P ^a   Zarrin, Ali A ^a,e   Brodeur, Peter H ^b   Alt, Frederick W ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^d NETHERLANDS CANCER INSTITUTE   (Netherlands)

^e GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; IMMUNOGLOBULIN HEAVY CHAIN; DEOXYRIBONUCLEASE I; TRANSCRIPTION FACTOR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; B LYMPHOCYTE; BINDING SITE; CELL MATURATION; CELL SELECTION; CONTROLLED STUDY; FLOW CYTOMETRY; GENE FREQUENCY; GENE LOCUS; GENETIC RECOMBINATION; HOMOZYGOSITY; MOUSE; NONHUMAN; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN BINDING; ANIMAL; CELL CULTURE; CELL DIFFERENTIATION; DNA FLANKING REGION; DRUG EFFECT; GENE DELETION; GENE REARRANGEMENT; GENETICS; GENOTYPE; LYMPHOCYTE; METABOLISM; MUTATION; PRE B LYMPHOCYTE; REGULATORY SEQUENCE; VDJ EXON;

MUS;

5' FLANKING REGION; ANIMALS; BINDING SITES; CELL DIFFERENTIATION; CELLS, CULTURED; DEOXYRIBONUCLEASE I; GENE REARRANGEMENT, B-LYMPHOCYTE, HEAVY CHAIN; GENOTYPE; IMMUNOGLOBULIN CLASS SWITCHING; IMMUNOGLOBULIN HEAVY CHAINS; LYMPHOCYTES; MICE; MUTATION; PRECURSOR CELLS, B-LYMPHOID; RECOMBINATION, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; SEQUENCE DELETION; TRANSCRIPTION FACTORS; VDJ EXONS;

EID: 78649734550     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0013992     Document Type: Article

References (20)

1. Perlot T, Alt FW (2008) Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus. Adv Immunol 99: 1-32.
2. Alt FW, Yancopoulos GD, Blackwell TK, Wood C, Thomas E, et al. (1984) Ordered rearrangement of immunoglobulin heavy chain variable region segments. EMBO J 3: 1209-1219.
3. Chowdhury D, Sen R (2001) Stepwise activation of the immunoglobulin mu heavy chain gene locus. EMBO J 20: 6394-6403.
4. Jung D, Giallourakis C, Mostoslavsky R, Alt FW (2006) Mechanism and control of V(D)J recombination at the immunoglobulin heavy chain locus. Annu Rev Immunol 24: 541-570.
5. Chaudhuri J, Basu U, Zarrin A, Yan C, Franco S, et al. (2007) Evolution of the immunoglobulin heavy chain class switch recombination mechanism. Adv Immunol 94: 157-214.
6. Sakai E, Bottaro A, Davidson L, Sleckman BP, Alt FW (1999) Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions. Proc Natl Acad Sci U S A 96: 1526-1531.
7. Perlot T, Alt FW, Bassing CH, Suh H, Pinaud E (2005) Elucidation of IgH intronic enhancer functions via germ-line deletion. Proc Natl Acad Sci U S A 102: 14362-14367.
8. Khamlichi AA, Pinaud E, Decourt C, Chauveau C, Cogne M (2000) The 39 IgH regulatory region: a complex structure in a search for a function. Adv Immunol 75: 317-345.
9. Pawlitzky I, Angeles CV, Siegel AM, Stanton ML, Riblet R, et al. (2006) Identification of a candidate regulatory element within the 59 flanking region of the mouse Igh locus defined by pro-B cell-specific hypersensitivity associated with binding of PU.1, Pax5, and E2A. J Immunol 176: 6839-6851.
10. Sleckman BP, Bardon CG, Ferrini R, Davidson L, Alt FW (1997) Function of the TCR alpha enhancer in alphabeta and gammadelta T cells. Immunity 7: 505-515.
11. Chen J, Lansford R, Stewart V, Young F, Alt FW (1993) RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development. Proc Natl Acad Sci U S A 90: 4528-4532.
12. Dudley DD, Sekiguchi J, Zhu C, Sadofsky MJ, Whitlow S, et al. (2003) Impaired V(D)J recombination and lymphocyte development in core RAG1-expressing mice. J Exp Med 198: 1439-1450.
13. Franco S, Gostissa M, Zha S, Lombard DB, Murphy MM, et al. (2006) H2AX prevents DNA breaks from progressing to chromosome breaks and translocations. Mol Cell 21: 201-214.
14. Hewitt SL, Farmer D, Marszalek K, Cadera E, Liang HE, et al. (2008) Association between the Igk and Igh immunoglobulin loci mediated by the 39 Igk enhancer induces 'decontraction' of the Igh locus in pre-B cells. Nat Immunol 9: 396-404.
15. Mostoslavsky R, Alt FW, Rajewsky K (2004) The lingering enigma of the allelic exclusion mechanism. Cell 118: 539-544.
16. Kosak ST, Skok JA, Medina KL, Riblet R, Le Beau MM, et al. (2002) Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. Science 296: 158-162.
17. Fuxa M, Skok J, Souabni A, Salvagiotto G, Roldan E, et al. (2004) Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavychain gene. Genes Dev 18: 411-422.
18. Yang Q, Riblet R, Schildkraut CL (2005) Sites that direct nuclear compartmentalization are near the 59 end of the mouse immunoglobulin heavy-chain locus. Mol Cell Biol 25: 6021-6030.
19. Reddy KL, Zullo JM, Bertolino E, Singh H (2008) Transcriptional repression mediated by repositioning of genes to the nuclear lamina. Nature 452: 243-247.
20. Jolly CJ, Klix N, Neuberger MS (1997) Rapid methods for the analysis of immunoglobulin gene hypermutation: application to transgenic and gene targeted mice. Nucleic Acids Res 25(10): 1913-1919.