Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria

Acta Tropica

Volumn 102, Issue 2, 2007, Pages 79-83

  de Monbrison, Frédérique ^a,b   Mihoubi, Ilhem ^c   Picot, Stéphane ^a,b  

^a EDOUARD HERRIOT HOSPITAL   (France)

^b UNIVERSITÉ LYON 1   (France)

^c Universite Mentouri Constantine   (Algeria)

Author keywords

Cutaneous leishmaniasis; Filter paper; Real time polymerase chain reaction; Species identification

Indexed keywords

BIOASSAY; IDENTIFICATION METHOD; LEISHMANIASIS; PARASITE; POLYMERASE CHAIN REACTION; PROTOZOAN;

ALGERIA; ANALYTIC METHOD; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN TISSUE; LEISHMANIA; LEISHMANIA DONOVANI; LEISHMANIA INFANTUM; LEISHMANIA MAJOR; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; SKIN DEFECT; SKIN LEISHMANIASIS;

ALGERIA; ANIMALS; DNA, PROTOZOAN; HUMANS; LEISHMANIA; LEISHMANIASIS, CUTANEOUS; POLYMERASE CHAIN REACTION;

AFRICA; ALGERIA; CONSTANTINE [ALGERIA]; NORTH AFRICA;

LEISHMANIA DONOVANI; LEISHMANIA INFANTUM; LEISHMANIA MAJOR; LEISHMANIA TROPICA;

EID: 34250192958     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2007.04.001     Document Type: Article

References (24)

1. Abdo M.G., Elamin W.M., Khalil E.A., and Mukhtar M.M. Antimony-resistant Leishmania donovaniin eastern Sudan: incidence and in vitro correlation. East. Mediterr. Health J. 9 (2003) 837-843
2. Ashford R.W. The leishmaniases as emerging and reemerging zoonoses. Int. J. Parasitol. 30 (2000) 1269-1281
3. Bensoussan E., Nasereddin A., Jonas F., Schnur L.F., and Jaffe C.L. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J. Clin. Microbiol. 44 (2006) 1435-1439
4. Croan D.G., Morrison D.A., and Ellis J.T. Evolution of the genus Leishmania revealed by comparison of DNA and RNA polymerase gene sequences. Mol. Biochem. Parasitol. 89 (1997) 149-159
5. Croft S.L., Sundar S., and Fairlamb A.H. Drug resistance in leishmaniasis. Clin. Microbiol. Rev. 19 (2006) 111-126
6. Desjeux P. The increase in risk factors for leishmaniasis worldwide. Trans. R. Soc. Trop. Med. Hyg. 95 (2001) 239-243
7. Gangneux J.P., Menotti J., Lorenzo F., Sarfati C., Blanche H., Bui H., Pratlong F., Garin Y.J., and Derouin F. Prospective value of PCR amplification and sequencing for diagnosis and typing of old world Leishmania infections in an area of nonendemicity. J. Clin. Microbiol. 41 (2003) 1419-1422
8. Guessous-Idrissi N., Chiheb S., Hamdani A., Riyad M., Bichichi M., Hamdani S., and Krimech A. Cutaneous leishmaniasis: an emerging epidemic focus of Leishmania tropica in north Morocco. Trans. R. Soc. Trop. Med. Hyg. 91 (1997) 660-663
9. Hadighi R., Mohebali M., Boucher P., Hajjaran H., Khamesipour A., and Ouellette M. Unresponsiveness to glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant Leishmania tropica parasites. PLoS Med. 3 (2006) e162
10. Harrat Z., Pratlong F., Belazzoug S., Dereure J., Deniau M., Rioux J.A., Belkaid M., and Dedet J.P. Leishmania infantum and L. major in Algeria. Trans. R. Soc. Trop. Med. Hyg. 90 (1996) 625-629
11. Jirku M., Zemanova E., Al-Jawabreh A., Schonian G., and Lukes J. Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica. Diagn. Microbiol. Infect. Dis. 55 (2006) 75-79
12. Mahboudi F., Abolhassani M., Tehrani S.R., Azimi M., and Asmar M. Differentiation of old and new world Leishmania species at complex and species levels by PCR. Scand. J. Infect. Dis. 34 (2002) 756-758
13. Marfurt J., Nasereddin A., Niederwieser I., Jaffe C.L., Beck H.P., and Felger I. Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J. Clin. Microbiol. 41 (2003) 3147-3153
14. Marques M.J., Volpini A.C., Genaro O., Mayrink W., and Romanha A.J. Simple form of clinical sample preservation and Leishmania DNA extraction from human lesions for diagnosis of American cutaneous leishmaniasis via polymerase chain reaction. Am. J. Trop. Med. Hyg. 65 (2001) 902-906
15. Mihoubi I., Monbrison de F., Romeuf N., Moulahem T., and Picot S. Outsourced real-time PCR diagnosis of cutaneous leishmaniasis in the outbreak region of Constantine. Algeria Med. Trop. 66 (2006) 39-44
16. Monbrison de F., Raynaud D., Latour-Fondanaiche C., Staal A., Favre S., Kaiser K., Peyron F., and Picot S. Real-time PCR for chloroquine sensitivity assay and for pfmdr1-pfcrt single nucleotide polymorphisms in Plasmodium falciparum. J. Clin. Microbiol. 54 (2003) 391-401
17. Nicolas L., Milon G., and Prina E. Rapid differentiation of old world Leishmania species by LightCycler polymerase chain reaction and melting curve analysis. J. Microbiol. Methods. 51 (2002) 295-299
18. Oliveira J.G., Novais F.O., de Oliveira C.I., da Cruz Junior A.C., Campos L.F., da Rocha A.V., Boaventura V., Noronha A., Costa J.M., and Barral A. Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis. Acta. Trop. 94 (2005) 55-59
19. Oliveira F.S., Pirmez C., Pires M.Q., Brazil R.P., and Pacheco R.S. PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil. Vet. Parasitol. 129 (2005) 219-227
20. Rodrigues E.H., Felinto de Brito M.E., Mendonca M.G., Werkhauser R.P., Coutinho E.M., Souza W.V., Militao de Albuquerque M de F., Jardim M.L., and Abath F.G. Evaluation of PCR for diagnosis of American cutaneous leishmaniasis in an area of endemicity in northeastern Brazil. J. Clin. Microbiol. 40 (2002) pp. 3572-3276
21. Salotra P., Sreenivas G., Pogue G.P., Lee N., Nakhasi H.L., Ramesh V., and Negi N.S. Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis. J. Clin. Microbiol. 39 (2001) 849-854
22. Silva d.E.S., Gontijo C.M., Pacheco R.da.S., and Brazil R.P. Diagnosis of human visceral leishmaniasis by PCR using blood samples spotted on filter paper. Genet. Mol. Res. 3 (2004) 251-257
23. Schulz A., Mellenthin K., Schonian G., Fleischer B., and Drosten C. Detection, differentiation, and quantitation of pathogenic Leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay. J. Clin. Microbiol. 41 (2003) 1529-1535
24. Wortmann G., Hochberg L., Houng H.H., Sweeney C., Zapor M., Aronson N., Weina P., and Ockenhouse C.F. Rapid identification of Leishmania complexes by a real-time PCR assay. Am. J. Trop. Med. Hyg. 73 (2005) 999-1004