The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting

Cellular and Molecular Biology Letters

Volumn 15, Issue 4, 2010, Pages 517-529

  Zhang, Yu ^a,b   Schedle, Andreas ^a   Matejka, Michael ^a   Rausch Fan, Xiaohui ^a   Andrukhov, Oleh ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b PEKING UNIVERSITY SCHOOL AND HOSPITAL OF STOMATOLOGY   (China)

Author keywords

Co culture; Differentiation; Endothelial cells; Osteoblasts; Proliferation

Indexed keywords

ALKALINE PHOSPHATASE; CD31 ANTIGEN; COLLAGEN TYPE 1; MESSENGER RNA; OSTEOCALCIN;

ARTICLE; CELL COUNT; CELL DIFFERENTIATION; CELL INTERACTION; CELL PROLIFERATION; CELL SEPARATION; CELL STIMULATION; CELL TYPE; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; OSTEOBLAST; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

EID: 78649693511     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-010-0026-0     Document Type: Article

References (36)

1. Olsen, B. R., Reginato, A. M. and Wang, W. Bone development. Annu. Rev. Cell Dev. Biol. 16 (2000) 191-220.
2. Sims, N. A. and Gooi, J. H. Bone remodeling: Multiple cellular interactions required for coupling of bone formation and resorption. Semin. Cell Dev. Biol. 19 (2008) 444-451.
3. Trueta, J. The role of the vessels in osteogenesis. J. Bone Joint Surg. 45B (1963) 402-418.
4. Collin-Osdoby, P. Role of vascular endothelial cells in bone biology. J. Cell. Biochem. 55 (1994) 304-309.
5. Wang, D. S., Miura, M., Demura, H. and Sato, K. Anabolic effects of 1, 25-dihydroxyvitamin D3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells. Endocrinology138 (1997) 2953-2962.
6. Streeten, E. A. and Brandi, M. L. Biology of bone endothelial cells. Bone Miner. 10 (1990) 85-94.
7. Clarkin, C. E., Emery, R. J., Pitsillides, A. A. and Wheeler-Jones, C. P. Evaluation of VEGF-mediated signaling in primary human cells reveals a paracrine action for VEGF in osteoblast-mediated crosstalk to endothelial cells. J. Cell. Physiol. 214 (2008) 537-544.
8. Bouletreau, P. J., Warren, S. M., Spector, J. A., Peled, Z. M., Gerrets, R. P., Greenwald, J. A. and Longaker, M. T. Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells: implications for fracture healing. Plast. Reconstr. Surg. 109 (2002) 2384-2397.
9. Finkenzeller, G., Arabatzis, G., Geyer, M., Wenger, A., Bannasch, H. and Stark, G. B. Gene expression profiling reveals platelet-derived growth factor receptor alpha as a target of cell contact-dependent gene regulation in an endothelial cell-osteoblast co-culture model. Tissue Eng. 12 (2006) 2889-2903.
10. Villars, F., Guillotin, B., Amédée, T., Dutoya, S., Bordenave, L., Bareille, R. and Amédée, J. Effect of HUVEC on human osteoprogenitor cell differentiation needs heterotypic gap junction communication. Am. J. Physiol. Cell Physiol. 282 (2002) C775-C785.
11. Villars, F., Bordenave, L., Bareille, R. and Amédée, J. Effect of human endothelial cells on human bone marrow stromal cell phenotype: role of VEGF? J. Cell. Biochem. 79 (2000) 672-685.
12. Grellier, M., Ferreira-Tojais, N., Bourget, C., Bareille, R., Guillemot, F. and Amédée, J. Role of vascular endothelial growth factor in the communication between human osteoprogenitors and endothelial cells. J. Cell. Biochem. 106 (2009) 390-398.
13. Kaigler, D., Krebsbach, P. H., West, E. R., Horger, K., Huang, Y. C. and Mooney, D. J. Endothelial cell modulation of bone marrow stromal cell osteogenic potential. FASEB J. 19 (2005) 665-667.
14. Guillotin, B., Bareille, R., Bourget, C., Bordenave, L. and Amédée, J. Interaction between human umbilical vein endothelial cells and human osteoprogenitors triggers pleiotropic effect that may support osteoblastic function. Bone42 (2008) 1080-1091.
15. Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M. and Herzenberg, L. A. The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford. Clin. Chem. 48 (2002) 1819-1827.
16. Herzenberg, L. A., Sweet R. G. and Herzenberg L. A. Fluorescence-activated cell sorting. Sci. Am. 234 1976 108-117.
17. Fuchs, S., Hofmann A., and C. J. Kirkpatrick, C. J. Microvessel-like structures from outgrowth endothelial cells from human peripheral blood in 2-dimensional and 3-dimensional co-cultures with osteoblastic lineage cells. Tissue Eng. 13 (2007) 2577-2588.
18. Rausch-Fan, X., Qu, Z., Wieland, M., Matejka, M. and Schedle, A. Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells (MG63) in response to titanium surfaces. Dent. Mater. 24 (2008) 102-110.
19. Pautke, C., Schieker, M., Tischer, T., Kolk, A., Neth, P., Mutschler, W. and Milz, S. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 24 (2004) 3743-3748.
20. Moreau, R., Aubin, R., Lapointe, J. Y. and Lajeunesse, D. Pharmacological and biochemical evidence for the regulation of osteocalcin secretion by potassium channels in human osteoblast-like MG-63 cells. J. Bone Miner. Res. 12 (1997) 1984-1992.
21. Boyan, B. D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D. L., Szmuckler-Moncler, S., Dean, D. D. and Schwartz, Z. Titanium surface roughness alters responsiveness of MG63 osteoblast-like cells to 1 alpha, 25-(OH)2D3. J. Biomed. Mater. Res. 39 (1998) 77-85.
22. Birch, M. A. and Skerry, T. M. Differential regulation of syndecan expression by osteosarcoma cell lines in response to cytokines but not osteotropic hormones. Bone24 (1999) 571-578.
23. Jonsson, K. B., Frost, A., Nilsson, O., Ljunghall, S. and Ljunggren, O. Three isolation techniques for primary culture of human osteoblast-like cells: a comparison. Acta Orthop. Scand. 70 (1999) 365-373.
24. Declercq, H., van der Vreken, N., De Maeyer, E., Verbeeck, R., Schacht, E., De Ridder, L. and Cornelissen, M. Isolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source. Biomaterials25 (2004) 757-768.
25. Martinez, M. E., del Campo, M. T., Medina, S., Sanchez, M., Sanchez-Cabezudo, M. J., Esbrit, P., Martinez, P., Moreno, I., Rodrigo, A., Garces, M. V. and Munuera, L. Influence of skeletal site of origin and donor age on osteoblastic cell growth and differentiation. Calcif. Tissue Int. 64 (1999) 280-286.
26. Unger, R. E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Bulnheim, U., Rychly, J. and Kirkpatrick, C. J. Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. Biomaterials28 (2007) 3965-3976.
27. Ng, K. W., Leong D. T. and Hutmacher, D. W. The challenge to measure cell proliferation in two and three dimensions. Tissue Eng. 11 (2005) 182-191.
28. Jones, L. J., Gray, M., Yue, S. T., Haugland, R. P. and Singer, V. L. Sensitive determination of cell number using the CyQUANT cell proliferation assay. J. Immunol. Methods254 (2001) 85-98.
29. Finkenzeller, G., Mehlhorn, A. T., Schmal, H. and Stark, G. B. Post-transcriptional regulation of osteoblastic platelet-derived growth factor receptor-alpha expression by co-cultured primary endothelial cells. Cells Tissues Organs (2010) DOI: 10. 1159/000276590.
30. Billiard, J., Moran, R. A., Whitley, M. Z., Chaterjee-Kishore, M., Gillis, K., Brown, E. L., Komm, B. S. and Bodine, P. V. Transcriptional profiling of human osteoblast differentiation. J. Cell. Biochem. 89 (2003) 389-400.
31. Stein, G. S. and Lian, J. B. Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr. Rev. 14 (1993) 424-442.
32. Aubin, J. E. Regulation of osteoblast formation and function. Rev. Endocr. Metab. Disord. 2 (2001) 81-94.
33. Lian, J. B. and Stein, G. S. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit. Rev. Oral. Biol. Med. 3 (1992) 269-305.
34. Ducy, P., Zhang, R., Geoffroy, V., Ridall, A. L. and Karsenty, R. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell89 (1997) 747-754.
35. Sun, H., Qu, Z., Guo, Y., Zang, G. and Yang, B. In vitro and in vivo effects of rat kidney vascular endothelial cells on osteogenesis of rat bone marrow mesenchymal stem cells growing on polylactide-glycoli acid (PLGA) scaffolds. Biomed. Eng. Online6 (2007) 41.
36. Meury, T., Verrier, S. and Alini, M. Human endothelial cells inhibit BMSC differentiation into mature osteoblasts in vitro by interfering with osterix expression. J. Cell. Biochem. 98 (2006) 992-1006.