Clostridium botulinum neurotoxin type B is heat-stable in milk and not inactivated by pasteurization

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 23, 2010, Pages 12557-12561

  Rasooly, Reuven ^a   Do, Paula M ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Clostridium botulinum; fluorescence resonance energy transfer (FRET); immunomagnetic beads; pasteurization; SNAP 25; synaptobrevin 2

Indexed keywords

CLOSTRIDIUM BOTULINUM; FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET); IMMUNOMAGNETIC BEADS; SNAP-25; SYNAPTOBREVIN;

ANIMALS; CLOSTRIDIUM; DRUG PRODUCTS; ENERGY TRANSFER; FLUORESCENCE; PASTEURIZATION; PEPTIDES; RESONANCE;

BIOASSAY;

ANIMALIA; CLOSTRIDIUM BOTULINUM;

BOTULINUM TOXIN; BOTULINUM TOXIN A; BOTULINUM TOXIN B;

ANALYSIS; ANIMAL; BOVINAE; CHEMISTRY; CLOSTRIDIUM BOTULINUM; EVALUATION STUDY; FOOD CONTAMINATION; GROWTH, DEVELOPMENT AND AGING; HEAT; METABOLISM; MICROBIOLOGY; MILK; PASTEURIZATION; PROCEDURES;

ANIMALS; BOTULINUM TOXINS; BOTULINUM TOXINS, TYPE A; CATTLE; CLOSTRIDIUM BOTULINUM; FOOD CONTAMINATION; HOT TEMPERATURE; MILK; PASTEURIZATION;

EID: 78649650524     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf1028398     Document Type: Article

References (16)

1. Betts, G. D.; Gaze, J. E. Growth and heat resistance of psychrotrophic Clostridium botulinum in relation to 'sous vide' product Food Control 1995, 6 (1) 57-63
2. Duda, J. J.; Slack, J. M. Toxin production in Clostridium botulinum as demonstrated by electron microscopy J. Bacteriol. 1969, 97 (2) 900-904
3. Siegel, L. S.; Metzger, J. F. Toxin production by Clostridium botulinum type A under various fermentation conditions Appl. Environ. Microbiol. 1979, 38 (4) 606-611
4. Sugiyama, H. Clostridium botulinum neurotoxin Microbiol. Rev. 1980, 44 (3) 419-448
5. Barr, J. R.; Moura, H.; Boyer, A. E.; Woolfitt, A. R.; Kalb, S. R.; Pavlopoulos, A.; McWilliams, L. G.; Schmidt, J. G.; Martinez, R. A.; Ashley, D. L. Botulinum neurotoxin detection and differentiation by mass spectrometry Emerging Infect. Dis. 2005, 11 (10) 1578-1583
6. Jacob, C. I. Botulinum neurotoxin type B-A rapid wrinkle reducer Semin. Cutaneous Med. Surg. 2003, 22 (2) 131-135
7. Rasooly, R.; Do, P. M. Development of an in vitro activity assay as an alternative to the mouse bioassay for Clostridium botulinum neurotoxin type A Appl. Environ. Microbiol. 2008, 74 (14) 4309-4313
8. Rasooly, R.; Stanker, L. H.; Carter, J. M.; Do, P. M.; Cheng, L. W.; He, X.; Brandon, D. L. Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay Int. J. Food Microbiol. 2008, 126 (1-2) 135-139
9. Boldt, G. E.; Kennedy, J. P.; Hixon, M. S.; McAllister, L. A.; Barbieri, J. T.; Tzipori, S.; Janda, K. D. Synthesis, characterization and development of a high-throughput methodology for the discovery of botulinum neurotoxin a inhibitors J. Comb. Chem. 2006, 8 (4) 513-521
10. Bona, C.; Dewals, B.; Wiggers, L.; Coudijzer, K.; Vanderplasschen, A.; Gillet, L. Short communication: Pasteurization of milk abolishes bovine herpesvirus 4 infectivity J. Dairy Sci. 2005, 88 (9) 3079-3083
11. Weingart, O. G.; Schreiber, T.; Mascher, C.; Pauly, D.; Dorner, M. B.; Berger, T. F.; Egger, C.; Gessler, F.; Loessner, M. J.; Avondet, M. A.; Dorner, B. G. The case of botulinum toxin in milk: experimental data Appl. Environ. Microbiol. 2010, 76 (10) 3293-3300
12. Wentzel, L. M.; Sterne, M.; Polson, A. High toxicity of pure botulinum type D toxin Nature 1950, 166 (4226) 739-740
13. Woodburn, M. J.; Schantz, E. J.; Rodriguez, J. Thermal inactivation of botulinurn toxins in canned salmon Home Econ. Res. J. 1979, 7 (3) 171-178
14. Loutfy, M. R.; Austin, J. W.; Blanchfield, B.; Fong, I. W. An outbreak of foodborne botulism in Ontario Can. J. Infect. Dis. 2003, 14 (4) 206-209
15. Cheng, L. W.; Onisko, B.; Johnson, E. A.; Reader, J. R.; Griffey, S. M.; Larson, A. E.; Tepp, W. H.; Stanker, L. H.; Brandon, D. L.; Carter, J. M. Effects of purification on the bioavailability of botulinum neurotoxin type A Toxicology 2008, 249 (2-3) 123-129
16. Ohishi, I.; Sugii, S.; Sakaguchi, G. Oral toxicities of Clostridium botulinum toxins in response to molecular size Infect. Immun. 1977, 16 (1) 107-109