Comparison of the histology, gene expression profile, and phenotype of cultured human limbal epithelial cells from different limbal regions

Investigative Ophthalmology and Visual Science

Volumn 50, Issue 11, 2009, Pages 5165-5172

  Utheim, Tor P ^a,b   Raeder, Sten ^a,b   Olstad, Ole K ^c   Utheim, Øygunn A ^b   de la Paz, Maria ^d   Cheng, Robert ^e   Huynh, Trang T ^a   Messelt, Edward ^f   Roald, Borghild ^g   Lyberg, Torstein ^a  

^a Central Institute for Industrial Research   (Norway)

^b Departments of Ophthalmology   (Norway)

^c Departments of Clinical Chemistry   (Norway)

^d INSTITUT UNIVERSITARI BARRAQUER   (Spain)

^e NATIONAL CANCER INSTITUTE   (United States)

^f UNIVERSITY OF OSLO   (Norway)

^g ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BETA1 INTEGRIN; BREAST CANCER RESISTANCE PROTEIN; GENE PRODUCT; NESTIN; PROTEIN OSR2; PROTEIN P53; PROTEIN RHOU; PROTEIN TRIM36; UNCLASSIFIED DRUG; UVOMORULIN; VIMENTIN;

ARTICLE; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL STRUCTURE; CONTROLLED STUDY; CORNEA CELL; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; GENOME; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LIMBAL EPITHELIUM CELL; MICROARRAY ANALYSIS; MICROSCOPY; MORPHOGENESIS; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THICKNESS; TRANSMISSION ELECTRON MICROSCOPY;

AGED; AGED, 80 AND OVER; BIOLOGICAL MARKERS; CARRIER PROTEINS; CELL COUNT; CELLS, CULTURED; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; IMMUNOENZYME TECHNIQUES; LIMBUS CORNEAE; MICROSCOPY, ELECTRON, TRANSMISSION; MIDDLE AGED; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHO GTP-BINDING PROTEINS; TRANSCRIPTION FACTORS;

EID: 78649331712     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.08-2884     Document Type: Article

References (52)

1. Pellegrini G, Traverso CE, Franzi AT, et al. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet. 1997;349:990-993.
2. Tsai RJ, Li LM, Chen JK. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med. 2000;343:86-93.
3. Schwab IR, Reyes M, Isseroff RR. Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea. 2000;19:421-426.
4. Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology. 2001;108:1569-1574.
5. Shimazaki J, Aiba M, Goto E, Kato N, Shimmura S, Tsubota K. Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders. Ophthalmology. 2002;109:1285-1290.
6. Ti SE, Grueterich M, Espana EM, Touhami A, Anderson DF, Tseng SC. Correlation of long term phenotypic and clinical outcomes following limbal epithelial transplantation cultivated on amniotic membrane in rabbits. Br J Ophthalmol. 2004;88:422-427.
7. Utheim TP, Raeder S, Utheim OA, et al. A novel method for preserving cultured limbal epithelial cells. Br J Ophthalmol. 2007; 91:797-800.
8. Raeder S, Utheim TP, Utheim OA, et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium. Invest Ophthalmol Vis Sci. 2007;48:5484-5493.
9. Tanioka H, Kawasaki S, Yamasaki K, et al. Establishment of a cultivated human conjunctival epithelium as an alternative tissue source for autologous corneal epithelial transplantation. Invest Ophthalmol Vis Sci. 2006;47:3820-3827.
10. Nakamura T, Endo K, Cooper LJ, et al. The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest Ophthalmol Vis Sci. 2003;44:106-116.
11. Yang X, Moldovan NI, Zhao Q, et al. Reconstruction of damaged cornea by autologous transplantation of epidermal adult stem cells. Mol Vis. 2008;14:1064-1070.
12. Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol. 2002;86:463-471.
13. Koizumi N, Cooper LJ, Fullwood NJ, et al. An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture. Invest Ophthalmol Vis Sci. 2002;43:2114-2121.
14. Koizumi N, Rigby H, Fullwood NJ, et al. Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells. Graefes Arch Clin Exp Ophthalmol. 2007;245:123-134.
15. Shortt AJ, Secker GA, Notara MD, et al. Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results. Surv Ophthalmol. 2007;52:483-502.
16. Schwab IR. Cultured corneal epithelia for ocular surface disease. Trans Am Ophthalmol Soc. 1999;97:891-986.
17. Davanger M, Evensen A. Role of the pericorneal papillary structure in renewal of corneal epithelium. Nature. 1971;229:560-561.
18. Cotsarelis G, Cheng SZ, Dong G, Sun TT, Lavker RM. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell. 1989;57:201-209.
19. Kinoshita S, Friend J, Thoft RA. Sex chromatin of donor corneal epithelium in rabbits. Invest Ophthalmol Vis Sci. 1981;21:434-441.
20. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol. 1986;103:49-62.
21. Goldberg MF, Bron AJ. Limbal palisades of Vogt. Trans Am Ophthalmol Soc. 1982;80:155-171.
22. Townsend WM. The limbal palisades of Vogt. Trans Am Ophthalmol Soc. 1991;89:721-756.
23. Wiley L, SundarRaj N, Sun TT, Thoft RA. Regional heterogeneity in human corneal and limbal epithelia: an immunohistochemical evaluation. Invest Ophthalmol Vis Sci. 1991;32:594-602.
24. Shanmuganathan VA, Foster T, Kulkarni BB, et al. Morphological characteristics of the limbal epithelial crypt. Br J Ophthalmol. 2007;91:514-519.
25. Buschke W, Friedenwald J, Fleischmann W. Studies on the miotic activity of the corneal epithelium: methods-the effects of Colchicine, Ether, Cocaine and Ephedrin. Bull Johns Hopkins Hosp. 1943:143-169.
26. Haskjold E, Refsum SB, Bjerknes R. Cell renewal of the rat corneal epithelium: a method to compare corresponding corneal areas from individual animals. Acta Ophthalmol. 1988;66:533-537.
27. Haskjold E, Bjerknes R, Refsum SB. Cell kinetics during healing of corneal epithelial wounds. Acta Ophthalmol. 1989;67:174-180.
28. Kaufman B, Gay H, Hollaender A. Distribution of mitoses in the corneal epithelium. Anat Res. 1944:161-178.
29. Lauweryns B, van den Oord JJ, Missotten L. The transitional zone between limbus and peripheral cornea: an immunohistochemical study. Invest Ophthalmol Vis Sci. 1993;34:1991-1999.
30. Shortt AJ, Secker GA, Munro PM, Khaw PT, Tuft SJ, Daniels JT. Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells. Stem Cells. 2007;25:1402-1409.
31. Pellegrini G, Golisano O, Paterna P, et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol. 1999;145:769-782.
32. Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol. 1997; 123:303-312.
33. Raeder S, Utheim TP, Utheim OA, et al. Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells. Acta Ophthalmol Scand. 2007;85:377-386.
34. http://linus.nci.nih.gov/BRB-ArrayTools.html/. National Center for Biotechnology Information, Bethesda, MD.
35. Ovstebo R, Haug KB, Lande K, Kierulf P. PCR-based calibration curves for studies of quantitative gene expression in human monocytes: development and evaluation. Clin Chem. 2003;49: 425-432.
36. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods. 2001;25:402-408.
37. Baldi P, Long AD. A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics. 2001;17:509-519.
38. Wright GW, Simon RM. A random variance model for detection of differential gene expression in small microarray experiments. Bioinformatics. 2003;19:2448-2455.
39. Short KM, Cox TC. Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. J Biol Chem. 2006;281:8970-8980.
40. Kawai S, Yamauchi M, Wakisaka S, Ooshima T, Amano A. Zincfinger transcription factor odd-skipped related 2 is one of the regulators in osteoblast proliferation and bone formation. J Bone Miner Res. 2007;22:1362-1372.
41. Lan Y, Ovitt CE, Cho ES, Maltby KM, Wang Q, Jiang R. Odd-skipped related 2 (Osr2) encodes a key intrinsic regulator of secondary palate growth and morphogenesis. Development. 2004;131:3207-3216.
42. Lan Y, Wang Q, Ovitt CE, Jiang R. A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development. Genesis. 2007;45:618-624.
43. Notarnicola C, Le GL, Fort P, Faure S, de Santa BP. Dynamic expression patterns of RhoV/Chp and RhoU/Wrch during chicken embryonic development. Dev Dyn. 2008;237:1165-1171.
44. Tao W, Pennica D, Xu L, Kalejta RF, Levine AJ. Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes Dev. 2001;15:1796-1807.
45. Ory S, Brazier H, Blangy A. Identification of a bipartite focal adhesion localization signal in RhoU/Wrch-1, a Rho family GTPase that regulates cell adhesion and migration. Biol Cell. 2007;99:701-716.
46. Klotz SA, Au YK, Misra RP. A partial-thickness epithelial defect increases the adherence of Pseudomonas aeruginosa to the cornea. Invest Ophthalmol Vis Sci. 1989;30:1069-1074.
47. Figueira EC, Di GN, Coroneo MT, Wakefield D. The phenotype of limbal epithelial stem cells. Invest Ophthalmol Vis Sci. 2007;48: 144-156.
48. Schlotzer-Schrehardt U, Kruse FE. Identification and characterization of limbal stem cells. Exp Eye Res. 2005;81:247-264.
49. Koizumi N, Fullwood NJ, Bairaktaris G, Inatomi T, Kinoshita S, Quantock AJ. Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane. Invest Ophthalmol Vis Sci. 2000;41:2506-2513.
50. James SE, Rowe A, Ilari L, Daya S, Martin R. The potential for eye bank limbal rings to generate cultured corneal epithelial allografts. Cornea. 2001;20:488-494.
51. Zito-Abbad E, Borderie VM, Baudrimont M, et al. Corneal epithelial cultures generated from organ-cultured limbal tissue: factors influencing epithelial cell growth. Curr Eye Res. 2006;31:391-399.
52. Shanmuganathan VA, Rotchford AP, Tullo AB, Joseph A, Zambrano I, Dua HS. Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking. Br J Ophthalmol. 2006;90:55-58.