Enrichment of Escherichia coli spheroplasts displaying scFv antibodies specific for antigens expressed on the human cell surface

Applied Microbiology and Biotechnology

Volumn 88, Issue 6, 2010, Pages 1385-1391

  Qiu, Jun Kang ^a   Jung, Sang Taek ^b   Georgiou, George ^b   Hang, Hai Ying ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b The University of Texas at Austin   (United States)

Author keywords

Anchored periplasmic expression; Antigens on the human cell surface; ScFv; Spheroplasts

Indexed keywords

ANCHORED PERIPLASMIC EXPRESSIONS; ANTIBODY LIBRARY; BACILLUS ANTHRACIS; CELL SURFACES; COMBINATORIAL LIBRARY; DISEASE MARKERS; DRUG TARGETS; HIGH AFFINITY LIGANDS; HUMAN CELLS; LIBRARY SCREENING; NEGATIVE CONTROL; POST-TRANSLATIONAL MODIFICATIONS; PROTECTIVE ANTIGENS; SCFV; SPHEROPLASTS;

ANTIGENS; BACTERIOLOGY; CELL MEMBRANES; ESCHERICHIA COLI; LIBRARIES;

ANTIBODIES;

BACTERIAL ANTIGEN; DIGOXIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY; ANTIGEN; CELL ORGANELLE; COLIFORM BACTERIUM; DRUG; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC MARKER; LIGAND; PROTEIN;

ANTIBODY ISOLATION; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; BACILLUS ANTHRACIS; BIOPANNING; CANCER CELL CULTURE; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; LUNG NON SMALL CELL CANCER; NONHUMAN; SPHEROPLAST;

ANTIGENS, BACTERIAL; BACTERIAL TOXINS; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION; HUMANS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; SPHEROPLASTS;

BACILLUS ANTHRACIS; ESCHERICHIA COLI;

EID: 78649330411     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2861-3     Document Type: Article

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