Purification of recombinant acyl-coenzyme a:cholesterol acyltransferase 1 (ACAT1) from H293 cells and binding studies between the enzyme and substrates using difference intrinsic fluorescence spectroscopy

Biochemistry

Volumn 49, Issue 46, 2010, Pages 9957-9963

  Chang, Catherine C Y ^a   Miyazaki, Akira ^b   Dong, Ruhong ^a   Kheirollah, Alireza ^a   Yu, Chunjiang ^c   Geng, Yong ^a   Higgs, Henry N ^a   Chang, Ta Yuan ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b SHOWA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL-COENZYME A:CHOLESTEROL ACYLTRANSFERASE; ALLOSTERIC ACTIVATOR; BINDING STUDIES; CHO CELL; CHOLESTERYL ESTERS; COENZYME A; DIASTEREOMERS; EXPRESSION LEVELS; FLUORESCENCE SPECTRA; KINETIC STUDY; MEMBRANE-BOUND ENZYMES; MIXED MICELLES; POSITIVE CHANGES; PURIFICATION PROCEDURES; PURIFIED PROTEIN; STEREOSPECIFICITY; STRUCTURAL CHANGE;

CHOLESTEROL; ENZYMES; ESTERS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PURIFICATION;

SUBSTRATES;

ACYL COENZYME A; CHOLESTEROL; CHOLESTEROL ACYLTRANSFERASE; RECOMBINANT ENZYME; STEAROYL COENZYME A;

ANIMAL CELL; ARTICLE; CELL LINE; CELL STRAIN H293; CHO CELL; ENZYME BINDING; ENZYME PURIFICATION; ENZYME SUBSTRATE; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE;

ANIMALS; CELLS, CULTURED; CHO CELLS; CHOLESTEROL; CRICETINAE; CRICETULUS; HUMANS; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; STEROL O-ACYLTRANSFERASE; SUBSTRATE SPECIFICITY;

EID: 78649278881     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1013936     Document Type: Article

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