A method for preparing primary retinal cell cultures for evaluating the neuroprotective and neuritogenic effect of factors on axotomized mature CNS neurons

Current Protocols in Neuroscience

Volumn , Issue SUPPL.53, 2010, Pages

  Grozdanov, Veselin ^a   Müller, Adrienne ^a   Sengottuvel, Vetrivel ^a   Leibinger, Marco ^a   Fischer, Dietmar ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

axon regeneration; neuroprotection; retinal cultures; retinal ganglion cell

Indexed keywords

BETA TUBULIN; BETAIII TUBULIN; CILIARY NEUROTROPHIC FACTOR; IMMUNOGLOBULIN G ANTIBODY; LAMININ; PARAFORMALDEHYDE; POLY DEXTRO LYSINE; POLYSORBATE 20; PROTEIN ANTIBODY; REAGENT; UNCLASSIFIED DRUG;

ANIMAL CELL; CELL ADHESION; CELL AGGREGATION; CELL COUNT; CELL SURVIVAL; CELL SUSPENSION; CENTRIFUGATION; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; ENUCLEATION; IMMUNOHISTOCHEMISTRY; METHODOLOGY; MICROBIAL CONTAMINATION; NERVE FIBER GROWTH; NERVE FIBER TRANSECTION; NEUROPROTECTION; NONHUMAN; PRIORITY JOURNAL; RAT; RETINA CELL; REVIEW; TIME;

ANIMALS; AXONS; AXOTOMY; CELL CULTURE TECHNIQUES; CELLS, CULTURED; NERVE REGENERATION; NEUROPROTECTIVE AGENTS; RATS; RETINAL GANGLION CELLS;

EID: 78449299506     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0322s53     Document Type: Review

References (22)

1. Berkelaar, M., Clarke, D.B., Wang, Y.C., Bray, G.M., and, Aguayo, A.J., 1994. Axotomy results in delayed death and apoptosis of retinal ganglion cells in adult rats. J. Neurosci. 14: 4368-4374.
2. Berry, M., Ahmed, Z., Lorber, B., Douglas, M., and, Logan, A., 2008. Regeneration of axons in the visual system. Restor. Neurol. Neurosci. 26: 147-174.
3. Fischer, D., Pavlidis, M., and, Thanos, S,. 2000. Cataractogenic lens injury prevents traumatic ganglion cell death and promotes axonal regeneration both in vivo and in culture. Invest. Ophthalmol. Vis. Sci. 41: 3943-3954.
4. Fischer, D., Heiduschka, P., and, Thanos, S., 2001. Lens-injury- stimulated axonal regeneration throughout the optic pathway of adult rats. Exp. Neurol. 172: 257-272.
5. Fischer, D., He, Z., and, Benowitz, L.I., 2004a. Counteracting the Nogo receptor enhances optic nerve regeneration if retinal ganglion cells are in an active growth state. J. Neurosci. 24: 1646-1651.
6. Fischer, D., Petkova, V., Thanos, S., and, Benowitz, L.I., 2004b. Switching mature retinal ganglion cells to a robust growth state in vivo: gene expression and synergy with RhoA inactivation. J. Neurosci. 24: 8726-8740.
7. Fischer, D., Hauk, T.G., Muller, A., and, Thanos, S., 2008. Crystallins of the beta/gamma-superfamily mimic the effects of lens injury and promote axon regeneration. Mol. Cell. Neurosci. 37: 471-479.
8. Fournier, A.E., Takizawa, B.T., and Strittmatter, S.M., 2003. Rho kinase inhibition enhances axonal regeneration in the injured CNS. J. Neurosci. 23: 1416-1423.
9. Gallagher, S., 2010. Digital image processing and analysis with ImageJ. Curr. Protoc. Essen. Lab. Tech. 3: A.3C.1 - A.3C.24.
10. GrandPre, T., Li, S., and, Strittmatter, S.M., 2002. Nogo-66 receptor antagonist peptide promotes axonal regeneration. Nature 417: 547-551.
11. Hauk, T., Mller, A., Lee, J., Schwendener, R., and, Fischer, D., 2008. Neuroprotective and axon growth promoting effects of intraocular inflammation do not depend on oncomodulin or the presence of large numbers of activated macrophages. Exp. Neurol. 209: 469-482.
12. Hauk, T.G., Leibinger, M., Muller, A., Andreadaki, N., Knippschild, U., and, Fischer, D., 2009. Stimulation of axon regeneration in the mature optic nerve by intravitreal application of the Toll-like receptor 2 agonist Pam 3 Cys. Invest. Ophthalmol. Vis. Sci. 51: 459-464.
13. Leaver, S.G., Cui, Q., Bernard, O., and, Harvey, A.R., 2006a. Cooperative effects of bcl-2 and AAV-mediated expression of CNTF on retinal ganglion cell survival and axonal regeneration in adult transgenic mice. Eur. J. Neurosci. 24: 3323-3332.
14. Leaver, S.G., Cui, Q., Plant, G.W., Arulpragasam, A., Hisheh, S., Verhaagen, J., and, Harvey, A.R., 2006b. AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells. Gene Ther. 13: 1328-1341.
15. Leibinger, M., Muller, A., Andreadaki, A., Hauk, T.G., Kirsch, M., and, Fischer, D., 2009. Neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and leukemia inhibitory factor. J. Neurosci. 29: 14334-14341.
16. Leon, S., Yin, Y., Nguyen, J., Irwin, N., and, Benowitz, L.I., 2000. Lens injury stimulates axon regeneration in the mature rat optic nerve. J. Neurosci. 20: 4615-4626.
17. Lorber, B., Berry, M., Douglas, M.R., Nakazawa, T., and, Logan, A., 2009. Activated retinal glia promote neurite outgrowth of retinal ganglion cells via apolipoprotein E. J. Neurosci. Res. 87: 2645-2652.
18. Muller, A., Hauk, T.G., and, Fischer, D., 2007. Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation. Brain 130: 3308-3320.
19. Muller, A, Hauk, TG, Leibinger, M, Marienfeld, R, and, Fischer, D., 2009. Exogenous CNTF stimulates axon regeneration of retinal ganglion cells partially via endogenous CNTF. Mol. Cell. Neurosci. 41: 233-246.
20. Rudge, J.S., and, Silver, J., 1990. Inhibition of neurite outgrowth on astroglial scars in vitro. J. Neurosci. 10: 3594-3603.
21. Yin, Y., Cui, Q., Li, Y., Irwin, N., Fischer, D., Harvey, A.R., and, Benowitz, L.I., 2003. Macrophage-derived factors stimulate optic nerve regeneration. J. Neurosci. 23: 2284-2293.
22. Yiu, G., and, He, Z., 2006. Glial inhibition of CNS axon regeneration. Nat. Rev. Neurosci. 7: 617-627.