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Volumn 1217, Issue 49, 2010, Pages 7749-7758
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Adsorptive detagging of poly-histidine tagged protein using hexa-histidine tagged exopeptidase
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Author keywords
Exopeptidase; Immobilized metal affinity chromatography; Maltose binding protein; Poly histidine fusion tag cleavage; Purification; Tag design
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Indexed keywords
EXOPEPTIDASE;
FUSION TAG;
IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY;
MALTOSE BINDING PROTEIN;
TAG DESIGN;
ADSORPTION;
AFFINITY CHROMATOGRAPHY;
AMINO ACIDS;
DESIGN;
MALTOSE;
PURIFICATION;
SULFUR COMPOUNDS;
PROTEINS;
ADSORBENT;
EXOPEPTIDASE;
HEXAHISTIDINE;
HYBRID PROTEIN;
IMIDAZOLE;
MALTOSE BINDING PROTEIN;
POLYHISTIDINE TAG;
PROTEINASE;
ADSORPTION;
ARTICLE;
CONTROLLED STUDY;
ENZYME ACTIVITY;
IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY;
INCUBATION TEMPERATURE;
ION EXCHANGE CHROMATOGRAPHY;
PRIORITY JOURNAL;
PROCESS OPTIMIZATION;
PROTEIN ANALYSIS;
PROTEIN PURIFICATION;
SEPARATION TECHNIQUE;
STRUCTURE ANALYSIS;
ADSORPTION;
ANIMALS;
CHROMATOGRAPHY, AFFINITY;
ESCHERICHIA COLI;
EXOPEPTIDASES;
HISTIDINE;
MALTOSE-BINDING PROTEINS;
OLIGOPEPTIDES;
RATS;
RECOMBINANT FUSION PROTEINS;
TEMPERATURE;
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EID: 78249273928
PISSN: 00219673
EISSN: None
Source Type: Journal
DOI: 10.1016/j.chroma.2010.10.050 Document Type: Article |
Times cited : (5)
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References (22)
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