Process intensification for the removal of poly-histidine fusion tags from recombinant proteins by an exopeptidase

Biotechnology Progress

Volumn 26, Issue 1, 2010, Pages 142-149

  Kuo, Wen Hui K ^a   Chase, Howard A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Exopeptidase; Immobilized metal affinity chromatography; Poly histidine fusion tag removal; Protein; Purification

Indexed keywords

CONCENTRATION OF; FUSION TAG; HISTIDINE TAGS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MALTOSE BINDING PROTEINS; MATRIX; PROCESS INTENSIFICATION; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; TAG REMOVAL;

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; METAL REFINING; PHOSPHATASES; PROTEINS; PURIFICATION; SULFUR COMPOUNDS;

METAL RECOVERY;

DIPEPTIDYL PEPTIDASE; EXOPEPTIDASE; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; OLIGOPEPTIDE; PERIPLASMIC BINDING PROTEIN; POLYHISTIDINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM;

CHROMATOGRAPHY, AFFINITY; DIPEPTIDYL-PEPTIDASES AND TRIPEPTIDYL-PEPTIDASES; EXOPEPTIDASES; HISTIDINE; OLIGOPEPTIDES; PERIPLASMIC BINDING PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 76849105927     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.305     Document Type: Article

References (16)

1. Arnau J, Lauritzen C, Petersen GE, Pedersen J. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr Purif. 2006;48:1-13.
2. Block H, Kubicek J, Labahn J, Roth U, Schäfer F. Production and comprehensive quality control of recombinant human Interleukin-1beta: a case study for a process development strategy. Protein Expr Purif. 2008;57:244-254.
3. Gaberc-Porekar V, Menart V. Potential for using histidine tags in purification of proteins at large scale. Chem Eng Technol. 2005;28:1306-1314.
4. Liu Q, Lin J, Liu M, Tao X, Wei D, Ma X, Yang S. Large scale preparation of recombinant human parathyroid hormone 1-84 from Escherichia coli. Protein Expr Purif. 2007;54:212-219.
5. Tan L, Kim D, Yoo I, Choe W. Harnessing metal ion affinity for the purification of plasmid DNA. Chem Eng Sci. 2007; 62:5809-5820.
6. Jenny RJ, Mann KG, Lundblad RL. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein Expr Purif. 2003;31:1-11.
7. Lauritzen C, Pedersen J, Madsen MT, Justesen J, Martensen PM, Dahl SW. Active recombinant rat dipeptidyl aminopeptidase I (cathepsin C) produced using the baculovirus expression system. Protein Expr Purif. 1998;14:434-442.
8. Pedersen J, Lauritzen C, Madsen MT, Weis Dahl S. Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Protein Expr Purif. 1999;15:389-400.
9. Abdullah N, Chase HA. Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption. Biotechnol Bioeng. 2005;92:501-513.
10. Kenig M, Peternel S, Gaberc-Porekar V, Menart V. Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins. J Chromatogr A. 2006;1101:293-306.
11. Schafer F, Shafer A, Steinert K. A highly specific system for efficient enzymatic removal of tags from recombinant proteins. J Biomolecular Techniques. 2002;13:158-171.
12. Tsukamoto H, Fukudome K, Kohara J, Nakatake H, Kimoto M. Preparation of recombinant murine tumor necrosis factor-alpha in Escherichia coli: a rapid method to remove tags from fusion proteins by thrombin-cleavage and ion-exchange chromatography. Protein Expr Purif. 2007;56:138-144.
13. Hefti MH, Van Vugt-Van der Toorn CJ, Dixon R, Vervoort J. A novel purification method for histidine-tagged proteins containing a thrombin cleavage site. Anal Biochem. 2001;295:180-185.
14. Bhikhabhai R, Sjoberg A, Hedkvist L, Galin M, Liljedahl P, Frigård T, Pettersson N, Nilsson M, Sigrell-Simon JA, Markeland-Johansson C. Production of milligram quantities of affinity tagged-proteins using automated multistep chromatographic purification. J Chromatogr A. 2005;1080:83-92.
15. Dian C, Eshaghi S, Urbig T, McSweeney S, Heijbel A, Salbert G, Birse D. Strategies for the purification and on-column cleavage of glutathione-S-transferase fusion target proteins. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;769:133-144.
16. Nilsson J, Ståhl S, Lundeberg J, Uhlén M, Nygren PA. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif. 1997;11:1-16.