Photochemical crosslinking of soluble wool keratins produces a mechanically stable biomaterial that supports cell adhesion and proliferation

Journal of Biomedical Materials Research - Part A

Volumn 95, Issue 3 A, 2010, Pages 901-911

  Sando, Lillian ^a   Kim, Misook ^a,b   Colgrave, Michelle L ^a   Ramshaw, John A M ^a   Werkmeister, Jerome A ^a   Elvin, Christopher M ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Adhesive; Di tyrosine; Fibroblasts; Keratin; Photo crosslinking

Indexed keywords

ADHESIVE STRENGTH; BIOCOMPATIBLE MATERIALS; BLUE LIGHT; CONSTITUENT PROTEINS; COVALENT CROSSLINKING; CROSS-LINK DENSITIES; CROSS-LINKED MATERIALS; CROSSLINKED; CROSSLINKS; DI-TYROSINE; HARD TISSUES; IN-VITRO; KERATIN; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MASS SPECTROMETRY ANALYSIS; MECHANICALLY STABLE; OXIDATIVE PROCESS; PHOTOCHEMICAL CROSS-LINKING; PHOTOCHEMICAL METHOD; PHOTOCROSS-LINKING; RUTHENIUM COMPLEXES; TISSUE ENGINEERING APPLICATIONS;

ADHESION; AMINO ACIDS; BIOMATERIALS; CELL ADHESION; CELL CULTURE; CHROMATOGRAPHY; FIBROBLASTS; GLYCEROL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUIDS; MASS SPECTROMETRY; RUTHENIUM; RUTHENIUM COMPOUNDS; SHRINKAGE; TENSILE STRENGTH; TISSUE; TISSUE ENGINEERING; WOOL; YARN;

CROSSLINKING;

BIOMATERIAL; DISULFIDE; GLYCEROL; KERATIN; RUTHENIUM COMPLEX;

ARTICLE; BLUE LIGHT; CELL ADHESION; CELL PROLIFERATION; CELL SWELLING; CROSS LINKING; ELASTICITY; FIBROBLAST; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROGEL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOTOCHEMICAL EFFICIENCY; PHOTOCHEMISTRY; PHOTOOXIDATION; PROTEIN ISOLATION; TENSILE STRENGTH; WOOL;

AMINO ACID SEQUENCE; ANIMALS; BIOCOMPATIBLE MATERIALS; CELL ADHESION; CELL PROLIFERATION; CROSS-LINKING REAGENTS; ELASTICITY; FIBROBLASTS; KERATINS; MATERIALS TESTING; MICE; NIH 3T3 CELLS; PHOTOCHEMICAL PROCESSES; TENSILE STRENGTH; TISSUE ENGINEERING; WOOL;

EID: 78249265611     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.32913     Document Type: Article

References (42)

1. Malafaya PB, Silva GA, Reis RL,. Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv Drug Deliv Rev 2007; 59: 207 -233.
2. Mano JF, Silva GA, Azevedo HS, Malafaya PB, Sousa RA, Silva SS, Boesel LF, Oliveira JM, Santos TC, Marques AP, Neves NM, Reis RL,. Natural origin biodegradable systems in tissue engineering and regenerative medicine: Present status and some moving trends. J R Soc Interface 2007; 4: 999 -1030.
3. Aluigi A, Varesano A, Montarsolo A, Vineis C, Ferrero F, Mazzuchetti G, Tonin C,. Electrospinning of keratin/poly(ethylene oxide) blend nanofibers. J Appl Polym Sci 2007; 104: 863 -870.
4. Reichl S,. Films based on human hair keratin as substrates for cell culture and tissue engineering. Biomaterials 2009; 30: 6854 -6866.
5. Fujii T, Murai S, Ohkawa K, Hirai T,. Effects of human hair and nail proteins and their films on rat mast cells. J Mater Sci Mater Med 2008; 19: 2335 -2342.
6. Crewther WG, Fraser RDB, Lennox FG, Lindley H,. The Chemistry of Keratins. New York: Academic Press; 1965.
7. Marshall RC, Gillespie JM,. Keratin proteins of wool, horn and hoof from sheep. Aust J Biol Sci 1977; 30: 389 -400.
8. Gillespie JM, Frenkel MJ,. The macroheterogeneity of type I tyrosine-rich proteins of merino wool. Aust J Biol Sci 1974; 27: 617 -627.
9. Yamauchi K, Yamauchi A, Kusunoki T, Kohda A, Konishi Y,. Preparation of stable aqueous solution of keratins, and physiochemical and biodegradational properties of films. J Biomed Mater Res 1996; 31: 439 -444.
10. Tachibana A, Furuta Y, Takeshima H, Tanabe T, Yamauchi K,. Fabrication of wool keratin sponge scaffolds for long-term cell cultivation. J Biotechnol 2002; 93: 165 -170.
11. Sierpinski P, Garrett J, Ma J, Apel P, Klorig D, Smith T, Koman LA, Atala A, Van Dyke M,. The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials 2008; 29: 118 -128.
12. Verma V, Verma P, Ray P, Ray AR,. Preparation of scaffolds from human hair proteins for tissue-engineering applications. Biomed Mater 2008; 3: 025007.
13. Aboushwareb T, Eberli D, Ward C, Broda C, Holcomb J, Atala A, Van Dyke M,. A keratin biomaterial gel hemostat derived from human hair: Evaluation in a rabbit model of lethal liver injury. J Biomed Mater Res B Appl Biomater 2009; 90: 45 -54.
14. Peplow PV, Dias GJ, Teixeira F, Kelly RJ,. Tissue reaction to matrices of reconstituted keratin polymer implanted subcutaneously in sheep. J Biomed Mater Res A 2009; 89: 255 -265.
15. Apel PJ, Garrett JP, Sierpinski P, Ma J, Atala A, Smith TL, Koman LA, Van Dyke ME,. Peripheral nerve regeneration using a keratin-based scaffold: Long-term functional and histological outcomes in a mouse model. J Hand Surg (Am) 2008; 33A: 1541 -1547.
16. Hill P, Brantley H, Van Dyke M,. Some properties of keratin biomaterials: Kerateines. Biomaterials 2010; 31: 585 -593.
17. Tanabe T, Okitsu N, Yamauchi K,. Fabrication and characterization of chemically crosslinked keratin films. Mat Sci Eng C 2004; 24: 441 -446.
18. Vasconcelos A, Freddi G, Cavaco-Paulo A,. Biodegradable materials based on silk fibroin and keratin. Biomacromolecules 2008; 9: 1299 -1305.
19. Zoccola M, Aluigi A, Vineis C, Tonin C, Ferrero F, Piacentino MG,. Study on cast membranes and electrospun nanofibers made from keratin/fibroin blends. Biomacromolecules 2008; 9: 2819 -2825.
20. Tachibana A, Kaneko S, Tanabe T, Yamauchi K,. Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation. Biomaterials 2005; 26: 297 -302.
21. Fujii T, Tanaka T, Ohkawa K,. Biomineralization of calcium phosphate on human hair protein film and formation of a novel hydroxyapatite-protein composite material. J Biomed Mater Res B Appl Biomater 2009; 91: 528 -536.
22. Dias GJ, Peplow PV, McLaughlin A, Teixeira F, Kelly RJ,. Biocompatibility and osseointegration of reconstituted keratin in an ovine model. J Biomed Mater Res A 2010; 92: 513 -520.
23. Yuan J, Shen J, Kang IK,. Fabrication of protein-doped PLA composite nanofibrous scaffolds for tissue engineering. Polym Int 2008; 57: 1188 -1193.
24. Raven DJ, Earland C, Little M,. Occurrence of dityrosine in tussah silk fibroin and keratin. Biochim Biophys Acta 1971; 251: 96 -99.
25. Stewart K, Spedding PL, Otterburn MS, Lewis DM,. Surface layer of wool. II. Dityrosine in wool. J Appl Polym Sci 1997; 66: 2365 -2376.
26. Andersen SO,. The cross-links in resilin identified as dityrosine and trityrosine. Biochim Biophys Acta 1964; 93: 213 -215.
27. Fancy DA, Kodadek T,. Chemistry for the analysis of protein-protein interactions: Rapid and efficient cross-linking triggered by long wavelength light. Proc Natl Acad Sci USA 1999; 96: 6020 -6024.
28. Elvin CM, Carr AG, Huson MG, Maxwell JM, Pearson RD, Vuocolo T, Liyou NE, Wong DC, Merritt DJ, Dixon NE,. Synthesis and properties of crosslinked recombinant pro-resilin. Nature 2005; 437: 999 -1002.
29. Elvin CM, Brownlee AG, Huson MG, Tebb TA, Kim M, Lyons RE, Vuocolo T, Liyou NE, Hughes TC, Ramshaw JA, Werkmeister JA,. The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant. Biomaterials 2009; 30: 2059 -2065.
30. Kongsuwan K, Josh P, Colgrave ML, Bagnall NH, Gough JE, Burns B, Pearson R,. Activation of several key components of the epidermal differentiation pathway in cattle following infestation with the cattle tick, Rhipicephalus (Boophilus) microplus. Int J Parasit 2010; 40: 499 -507.
31. Treloar LRG,. The Physics of Rubber Elasticity. Oxford: Clarendon Press; 1975.
32. Aaron BB, Gosline JM,. Elastin as a random-network elastomer: A mechanical and optical analysis of single elastin fibers. Biopolymers 1981; 20: 1247 -1260.
33. Corfield MC, Robson A, Skinner B,. The amino acid compositions of three fractions from oxidized wool. Biochem J 1958; 68: 348 -352.
34. Mies HH, Zahn H,. Chromatographic and electrophoretic investigations of the properties of unprotected low-sulfur wool kerateins. J Chromatogr 1987; 405: 365 -370.
35. Fraser RDB, MacRae TP, Sparrow LG, Parry DAD,. Disulphide bonding in α-keratin. Int J Biol Macromol 1988; 10: 106 -112.
36. Malencik DA, Anderson SR,. Dityrosine as a product of oxidative stress and fluorescent probe. Amino Acids 2003; 25: 233 -247.
37. Kuznetsova N, Chi SL, Leikin S,. Sugars and polyols inhibit fibrillogenesis of type I collagen by disrupting hydrogen-bonded water bridges between the helices. Biochemistry 1998; 37: 11888 -11895.
38. Leikin S, Rau DC, Parsegian VA,. Temperature-favoured assembly of collagen is driven by hydrophilic not hydrophobic interactions. Nat Struct Biol 1995; 2: 205 -210.
39. Liu B,. Fabrication and evaluation of a biodegradable proanthocyanidin-crosslinked gelatin conduit in peripheral nerve repair. J Biomed Mater Res A 2008; 87: 1092 -1102.
40. Baker EN, Hubbard RE,. Hydrogen bonding in globular proteins. Prog Biophys Mol Biol 1984; 44: 97 -179.
41. Tamada Y, Ikada Y,. Fibroblast growth on polymer surfaces and biosynthesis of collagen. J Biomed Mater Res A 1994; 28: 783 -789.
42. Groth T, Altankov G,. Studies on cell-biomaterial interaction role of tyrosine phosphorylation during fibroblast spreading on surfaces varying in wettability. Biomaterials 1996; 17: 1227 -1234.