Development and validation of four real-time quantitative RT-PCRs specific for the positive or negative strands of a bisegmented dsRNA viral genome

Journal of Virological Methods

Volumn 170, Issue 1-2, 2010, Pages 1-8

  Escaffre, O ^a   Queguiner, M ^a   Eterradossi, N ^a  

^a FRENCH AGENCY FOR FOOD   (France)

Author keywords

Birnavirus; Double stranded RNA; Infectious bursal disease virus; Quantitative tagged real time RT PCR; Strand specificity

Indexed keywords

CESIUM CHLORIDE; COMPLEMENTARY DNA; DOUBLE STRANDED RNA; SINGLE STRANDED RNA;

ACCURACY; ANIMAL CELL; ARTICLE; AVIBIRNAVIRUS; BIRNAVIRIDAE; CONTROLLED STUDY; DNA STRAND; DNA SYNTHESIS; DNA TEMPLATE; EMBRYO; IN VITRO STUDY; INFECTIOUS BURSAL DISEASE VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RNA ANALYSIS; RNA PURIFICATION; VALIDATION STUDY; VIRUS GENOME;

ANIMALS; BIRNAVIRIDAE INFECTIONS; CELLS, CULTURED; CESIUM; CHICK EMBRYO; CHLORIDES; DNA PRIMERS; GENOME, VIRAL; INFECTIOUS BURSAL DISEASE VIRUS; MICROSCOPY, ELECTRON; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, DOUBLE-STRANDED; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

AVIBIRNAVIRUS; BIRNAVIRIDAE; INFECTIOUS BURSAL DISEASE VIRUS;

EID: 78249242102     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.07.009     Document Type: Article

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