C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli

Journal of Biotechnology

Volumn 150, Issue 3, 2010, Pages 408-416

  Ribitsch, D ^a   Karl, W ^a   Birner Gruenberger, R ^a,d   Gruber, K ^a,c   Eiteljoerg, I ^a   Remler, P ^a   Wieland, S ^e   Siegert, P ^e   Maurer, K H ^e   Schwab, H ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c UNIVERSITY OF GRAZ   (Austria)

^d MEDICAL UNIVERSITY OF GRAZ   (Austria)

^e HENKEL KGAA   (Germany)

Author keywords

C terminal domain; Detergents; Protease; Xanthomonadales

Indexed keywords

ALKALINE PROTEASE; C-TERMINAL DOMAINS; C-TERMINAL TRUNCATION; CATALYTIC DOMAINS; CLEAVAGE PREFERENCE; DETERGENT ENZYMES; DETERGENT PROTEASE; E. COLI; EXTRACELLULAR; HIGH POTENTIAL; METAGENOMES; NATIVE ENZYMES; OVER-EXPRESSION; PEPTIDE FRAGMENTS; POTENTIAL APPLICATIONS; PROTEASE; SERINE PROTEASE; SPECIFIC ACTIVITY; STENOTROPHOMONAS MALTOPHILIA; STRUCTURAL MODIFICATIONS; TWO-DOMAIN STRUCTURES; XANTHOMONADALES; XANTHOMONAS CAMPESTRIS;

AMINO ACIDS; ENZYME ACTIVITY; ESCHERICHIA COLI; SOAPS (DETERGENTS);

ENZYMES;

C477 ENZYME; HP70 ENZYME; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; INSULIN BINDING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METAGENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; METAGENOME; MODELS, MOLECULAR; MOLECULAR SEQUENCE ANNOTATION; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SERINE PROTEASES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STENOTROPHOMONAS MALTOPHILIA; TEMPERATURE; XANTHOMONAS CAMPESTRIS;

ESCHERICHIA COLI; STENOTROPHOMONAS MALTOPHILIA; XANTHOMONADALES; XANTHOMONAS CAMPESTRIS;

EID: 78149468946     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.09.947     Document Type: Article

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