Characteristics of the m2000 automated sample preparation and multiplex real-time PCR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

Journal of Clinical Microbiology

Volumn 45, Issue 3, 2007, Pages 747-751

  Marshall, R ^a   Chernesky, M ^b   Jang, D ^b   Hook, E W ^c   Cartwright, C P ^d   Howell Adams, B ^a   Ho, S ^a   Welk, J ^a   Lai Zhang, J ^a   Brashear, J ^a   Diedrich, B ^a   Otis, K ^a   Webb, E ^a   Robinson, J ^a   Yu, H ^a  

^a Abbott Molecular Inc   (United States)

^b MCMASTER UNIVERSITY   (Canada)

^c UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^d UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ANALYTIC METHOD; ARTICLE; AUTOMATION; BACTERIAL GENOME; BACTERIUM DETECTION; BACTERIUM ISOLATE; CHLAMYDIA TRACHOMATIS; CROSS REACTION; EVALUATION; FEASIBILITY STUDY; GENE AMPLIFICATION; GENE TARGETING; LABORATORY; NEISSERIA GONORRHOEAE; NONHUMAN; PLASMID; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

AUTOMATION; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; FEMALE; FEMALE UROGENITAL DISEASES; GONORRHEA; HUMANS; MAGNETICS; MALE; MALE UROGENITAL DISEASES; NEISSERIA GONORRHOEAE; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

CHLAMYDIA; CHLAMYDIA TRACHOMATIS; NEISSERIA; NEISSERIA GONORRHOEAE;

EID: 33947225732     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01956-06     Document Type: Article

References (20)

1. Cates, W., and J. N. Wasserheit. 1991. Genital Chlamydia infections; epidemiology and reproductive sequelae. Am. J. Obstet. Gynecol. 164:1771-1781.
2. Chernesky, M., D. Jang, H. Lee, J. D. Burczak, H. Hu, J. Sellors, S. J. Tomazic-Allen, and J. B. Mahony. 1994. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J. Clin. Microbiol. 32:2682-2685.
3. Chernesky, M., D. Jang, K. Luinstra, S. Chong, M. Smieja, W. Cai, B. Hayhoe, E. Portillo, C. MacRitchie, C. Main, and R. Ewert. 2006. High analytical sensitivity and low rates of inhibition may contribute to detection of Chlamydia trachomatis in significantly more women by the APTIMA Combo 2 assay. J. Clin. Microbiol. 44:400-405.
4. Chong, S., D. Jang, X. Song, J. Mahony, A. Petrich, P. Barriga, and M. Chernesky. 2003. Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA Combo 2 assay when testing for inhibitors. J. Clin. Microbiol. 41:778-782.
5. Cosentino, L. A., D. V. Landers, and S. L. Hillier. 2003. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by strand displacement amplification and relevance of the amplification control for use with vaginal swab specimens. J. Clin. Microbiol. 41:3592-3596.
6. Culler, E. E., A. M. Caliendo, and F. S. Nolte. 2003. Reproducibility of positive test results in the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 41:3911-3914.
7. Farrell, D. 1999. Evaluation of AMPLICOR Neisseria gonorrhoeae using cppB nested PCR and 16S rRNA PCR. J. Clin. Microbiol. 37:386-390.
8. Gaydos, C. A., T. C. Quinn, D. Willis, A. Weissfeld, E. W. Hook, D. H. Martin, D. C. Ferrero, and J. Schachter. 2003. Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J. Clin. Microbiol. 41:304-309.
9. Hanson, K. L., and C. P. Cartwright. 2001. Evaluation of an automated liquid-handling system (Tecan Genesis RSP 100) in the Abbott LCx assay for Chlamydia trachomatis. J. Clin. Microbiol. 39:1975-1977.
10. Mahony, J. B., S. Chong, D. Jang, K. Luinstra, M. Faught, D. Dalby, J. Sellors, and M. A. Chernesky. 1998. Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplifications: identification of urinary substances associated with inhibition and removal of inhibitory activity. J. Clin. Microbiol. 36:3122-3126.
11. Martin, D. H., C. Cammarata, B. Van der Pol, R. B. Jones, T. C. Quinn, C. A. Gaydos, K. Crotchfelt, J. Schachter, J. Moncada, D. Jungkind, B. Turner, and C. Peyton. 2000. Multicenter evaluation of AMPLICOR and automated COBAS AMPLICOR CT/NG tests for Neisseria gonorrhoeae. J. Clin. Microbiol. 38:3544-3549.
12. Meyer, T. F., C. P. Gibbs, and R. Hass. 1990. Variation and control of protein expression in Neisseria. Annu. Rev. Microbiol. 44:451-477.
13. Palmer, L., and S. Falkow. 1986. A common plasmid of Chlamydia trachomatis. Plasmid 16:52-62.
14. Schachter, J., E. W. Hook III, W. M. McCormack, T. C. Quinn, M. Chernesky, S. Chong, J. I. Girdner, P. B. Dixon, L. DeMeo, E. Williams, A. Cullen, and A. Lorincz. 1999. Ability of the Digene hybrid capture II test to identify Chlamydia trachomatis and Neisseria gonorrhoeae in cervical specimens. J. Clin. Microbiol. 37:3668-3671.
15. Schachter, J., W. M. McCormack, M. A. Chernesky, D. H. Martin, B. Van der Pol, P. A. Rice, E. W. Hook III, W. E. Stamm, T. C. Quinn, and J. M. Chow. 2003. Vaginal swabs are appropriate specimens for diagnosis of genital tract infection with Chlamydia trachomatis. J. Clin. Microbiol. 41:3784-3789.
16. Van der Pol, B., T. C. Quinn, C. A. Gaydos, K. Crotchfelt, J. Schachter, J. Moncada, D. Jungkind, D. H. Martin, B. Turner, C. Peyton, and R. B. Jones. 2000. Multicenter evaluation of the AMPLICOR and automated COBAS AMPLICOR CT/NG tests for detection of Chlamydia trachomatis. J. Clin. Microbiol. 38:1105-1112.
17. Van der Pol, B., D. V. Ferrero, L. Buck-Barrington, E. Hook III, C. Lenderman, T. Quinn, C. A. Gaydos, J. Lovchik, J. Schacter, J. Moncada, G. Hall, M. J. Tuohy, and R. B. Jones. 2001. Multicenter evaluation of the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J. Clin. Microbiol. 39:1008-1016.
18. Van der Pol, B., J. A. Williams, N. J. Smith, B. E. Batteiger, A. P. Cullen, H. Erdman, T. Edens, K. Davis, H. Salim-Hammad, V. W. Chou, L. Scearce, J. Blutman, and W. J. Payne. 2002. Evaluation of the Digene hybrid capture II assay with the rapid capture system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 40:3558-3564.
19. van Dyck, E., M. Ieven, S. Pattyn, L. van Damme, and M. Laga. 2001. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests. J. Clin. Microbiol. 39:1751-1756.
20. Vincelette, J., J. Schirm, M. Bogard, A.-M. Bourgault, D. S. Luijt, A. Bianchi, P. C. van Voorst Vader, A. Butcher, and M. Rosenstraus. 1999. Multicenter evaluation of the fully automated COBAS AMPLICOR PCR test for detection of Chlamydia trachomatis in urogenital specimens. J. Clin. Microbiol. 37:74-80.