Characterization of a thermostable xylanase from an alkaliphilic Bacillus sp

Biotechnology Letters

Volumn 32, Issue 12, 2010, Pages 1915-1920

  Zhang, Guimin ^a,b   Mao, Liangwei ^b   Zhao, Yueju ^a   Xue, Yanfen ^a   Ma, Yanhe ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b HUBEI UNIVERSITY   (China)

Author keywords

Bacillus sp.; Heterologous expression; Pichia pastoris; Thermostable xylanase

Indexed keywords

AMINO ACID SEQUENCE; BACILLUS SP; GLYCOSYLATED; HETEROLOGOUS EXPRESSION; INHIBITION EFFECT; N-GLYCOSYLATION SITES; OPTIMUM ACTIVITY; PICHIA PASTORIS; RESIDUAL ACTIVITY; SPECIFIC ACTIVITY; THERMOSTABLE XYLANASE; XYLANASES;

AMINO ACIDS; BACTERIOLOGY; ENZYME INHIBITION; ENZYMES;

ENZYME ACTIVITY;

BACTERIAL DNA; RECOMBINANT PROTEIN; XYLAN; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; BACILLUS; CHEMISTRY; DNA SEQUENCE; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PICHIA; SEQUENCE HOMOLOGY; TEMPERATURE; TIME;

BACILLUS; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME STABILITY; GENE EXPRESSION; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS; XYLANS; XYLOSIDASES;

BACILLUS HALODURANS; BACILLUS SP.; PICHIA PASTORIS;

EID: 78149416320     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0372-z     Document Type: Article

References (14)

1. Berrin JG, Juge N (2008) Factors affecting xylanase functionality in the degradation of arabinoxylans. Biotechnol Lett 30: 1139-1150.
2. Chang P, Tsai WS, Tsai CL et al (2004) Cloning and characterization of two thermostable xylanases from an alkaliphilic Bacillus firmus. Biochem Biophys Res Commun 319: 1017-1025.
3. Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29: 3-23.
4. Feng L, Wang W, Cheng J et al (2007) Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. Proc Natl Acad Sci USA 104: 5602-5607.
5. Gat O, Lapidot A, Alchanati I et al (1994) Cloning and DNA sequence of the gene coding for Bacillus stearothermophilus T-6 xylanase. Appl Environ Microbiol 60: 1889-1896.
6. Gupta N, Vohra RMV, Hoondal GS (1992) A thermostable extracellular xylanase from alkalophilic Bacillus sp. NG-27. Biotechnol Lett 14: 1045-1046.
7. Henrissat B (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280(Pt 2): 309-316.
8. Ma Y, Xue Y, Dou Y et al (2004) Characterization and gene cloning of a novel beta-mannanase from alkaliphilic Bacillus sp. N16-5. Extremophiles 8: 447-454.
9. Mamo G, Delgado O, Martinez A et al (2006) Cloning, sequence analysis, and expression of a gene encoding an endoxylanase from Bacillus halodurans S7. Mol Biotechnol 33: 149-159.
10. Mamo G, Thunnissen M, Hatti-Kaul R et al (2009) An alkaline active xylanase: insights into mechanisms of high pH catalytic adaptation. Biochimie 91: 1187-1196.
11. Manikandan K, Bhardwaj A, Gupta N et al (2006) Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: Structural insights into alkalophilicity and implications for adaptation to polyextreme conditions. Protein Sci 15: 1951-1960.
12. Nishimoto M, Fushinobu S, Miyanaga A et al (2007) Molecular anatomy of the alkaliphilic xylanase from Bacillus halodurans C-125. J Biochem 141: 709-717.
13. Zhang GM, Hu Y, Zhuang YH et al (2006) Molecular cloning and expression in Pichia pastoris of a xylanase gene from Bacillus pumilus HBP8. Biocat Biotrans 24: 371-379.
14. Zhang GM, Huang J, Huang GR et al (2007) Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina. Appl Microbiol Biotechnol 74: 339-346.